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Biosafe alternative to xylene: A comparative study.

Negi A, Puri A, Gupta R, Chauhan I, Nangia R, Sachdeva A - J Oral Maxillofac Pathol (2013)

Bottom Line: Sixty sections of 4 μm were obtained from 30 formalin-fixed paraffin-embedded (FFPE) tissues and were considered in two different groups, groups A and B.Slides in group A were stained with routine H and E staining procedure; whereas, slides in group B were stained using 1.7% DWS as a deparaffinizing agent.Wilcoxon matched-pairs signed rank test was used to calculate the test of significance (P-value significant at ≤0.05). 1.7% DWS was found to be an effective alternative deparaffinizing agent to xylene and meanwhile facilitating as less biohazardous, economical and a faster deparaffinizing agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Himachal Institute of Dental Sciences, Paonta Sahib, Sirmour, Himachal Pradesh, India.

ABSTRACT

Background: Xylene in one of the non-substitutable chemical used in histology laboratories. However, it is known to have many toxic effects. The toxic effects of xylene include heart and kidney injuries, some fatal blood dyscrasia and other less dangerous problems, such as skin erythema, drying, scaling and secondary infections. The exposure and handling of xylene is maximum during deparaffinizing tissue sections.

Aims: The aim of this study was to evaluate the efficacy of 1.7% dishwashing soap (DWS) solution as a deparaffinizing agent for hematoxylin and eosin (H and E) staining and compare it with xylene.

Materials and methods: Sixty sections of 4 μm were obtained from 30 formalin-fixed paraffin-embedded (FFPE) tissues and were considered in two different groups, groups A and B. Slides in group A were stained with routine H and E staining procedure; whereas, slides in group B were stained using 1.7% DWS as a deparaffinizing agent.

Statistical analysis used: Wilcoxon matched-pairs signed rank test was used to calculate the test of significance (P-value significant at ≤0.05).

Results and conclusion: 1.7% DWS was found to be an effective alternative deparaffinizing agent to xylene and meanwhile facilitating as less biohazardous, economical and a faster deparaffinizing agent.

No MeSH data available.


Related in: MedlinePlus

Comparison of photomicrographs of sections stained with routine H and E staining in (a) epithelium and connective tissue and (c) Ameloblastoma (×100), with xylene free H and E staining in (b) epithelium and connective tissue and (d) Ameloblastoma (×100)
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Figure 1: Comparison of photomicrographs of sections stained with routine H and E staining in (a) epithelium and connective tissue and (c) Ameloblastoma (×100), with xylene free H and E staining in (b) epithelium and connective tissue and (d) Ameloblastoma (×100)

Mentions: The results for all the grading criteria were found to be statistically insignificant with slightly higher mean values for xylene free staining. Also the percentage of adequacy of diagnosis was found to be higher for O1 and O2 (86 and 100%, respectively) for xylene free staining as compared to routine H and E staining (73 and 86%) [Tables 3–8, Figures 1 and 2].


Biosafe alternative to xylene: A comparative study.

Negi A, Puri A, Gupta R, Chauhan I, Nangia R, Sachdeva A - J Oral Maxillofac Pathol (2013)

Comparison of photomicrographs of sections stained with routine H and E staining in (a) epithelium and connective tissue and (c) Ameloblastoma (×100), with xylene free H and E staining in (b) epithelium and connective tissue and (d) Ameloblastoma (×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927336&req=5

Figure 1: Comparison of photomicrographs of sections stained with routine H and E staining in (a) epithelium and connective tissue and (c) Ameloblastoma (×100), with xylene free H and E staining in (b) epithelium and connective tissue and (d) Ameloblastoma (×100)
Mentions: The results for all the grading criteria were found to be statistically insignificant with slightly higher mean values for xylene free staining. Also the percentage of adequacy of diagnosis was found to be higher for O1 and O2 (86 and 100%, respectively) for xylene free staining as compared to routine H and E staining (73 and 86%) [Tables 3–8, Figures 1 and 2].

Bottom Line: Sixty sections of 4 μm were obtained from 30 formalin-fixed paraffin-embedded (FFPE) tissues and were considered in two different groups, groups A and B.Slides in group A were stained with routine H and E staining procedure; whereas, slides in group B were stained using 1.7% DWS as a deparaffinizing agent.Wilcoxon matched-pairs signed rank test was used to calculate the test of significance (P-value significant at ≤0.05). 1.7% DWS was found to be an effective alternative deparaffinizing agent to xylene and meanwhile facilitating as less biohazardous, economical and a faster deparaffinizing agent.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Pathology, Himachal Institute of Dental Sciences, Paonta Sahib, Sirmour, Himachal Pradesh, India.

ABSTRACT

Background: Xylene in one of the non-substitutable chemical used in histology laboratories. However, it is known to have many toxic effects. The toxic effects of xylene include heart and kidney injuries, some fatal blood dyscrasia and other less dangerous problems, such as skin erythema, drying, scaling and secondary infections. The exposure and handling of xylene is maximum during deparaffinizing tissue sections.

Aims: The aim of this study was to evaluate the efficacy of 1.7% dishwashing soap (DWS) solution as a deparaffinizing agent for hematoxylin and eosin (H and E) staining and compare it with xylene.

Materials and methods: Sixty sections of 4 μm were obtained from 30 formalin-fixed paraffin-embedded (FFPE) tissues and were considered in two different groups, groups A and B. Slides in group A were stained with routine H and E staining procedure; whereas, slides in group B were stained using 1.7% DWS as a deparaffinizing agent.

Statistical analysis used: Wilcoxon matched-pairs signed rank test was used to calculate the test of significance (P-value significant at ≤0.05).

Results and conclusion: 1.7% DWS was found to be an effective alternative deparaffinizing agent to xylene and meanwhile facilitating as less biohazardous, economical and a faster deparaffinizing agent.

No MeSH data available.


Related in: MedlinePlus