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Cyclin D1 an early biomarker in oral carcinogenesis.

Ramakrishna A, Shreedhar B, Narayan T, Mohanty L, Shenoy S, Jamadar S - J Oral Maxillofac Pathol (2013)

Bottom Line: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant.Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, College of Dental Sciences, Davangere, Karnataka, India.

ABSTRACT

Background: Dysregulation of cell cycle is a fundamental hallmark of cancer progression. Cyclin D1, part of complex molecular system regulating G1-S point of cell cycle is overexpressed in variety of tumors.

Aims: To look for its immunohistochemical expression in clinically normal mucosa from patients with and without tobacco habits, leukoplakia; and correlate its expression to individual atypical morphologic features, as seen in hematoxylin and eosin (H and E) sections of leukoplakia exhibiting dysplasia.

Materials and methods: We examined the expression of cyclin D1 in immunohistochemically stained sections of 15 normal buccal mucosa without any habits (group 1), 30 clinically normal mucosa from tobacco habituιs (group 2) and 30 leukoplakias exhibiting dysplasias (group 3). Descriptive statistical analysis performed. Results presented on Mean ± Standard deviation and in number (%). Adjusted Wald 95% Confidence Interval (CI) computed, percentages of morphological features assessed by Laplace estimate. Mann-Whitney U, Kruskal-Wallis test used to find the percentage expression of cyclin D1.

Results: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant. Generally expression of cyclin D1 was confined to lower one-third of epithelium and was highest in mild dysplasias. Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.

Conclusion: The altered pattern of cyclin D1 expression here may be an early event in conversion of normal epithelium into dysplastic epithelium and may serve as a biomarker of oral carcinogenesis. Its expression may be increased in tobacco habitués. Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph showing basilar hyperplasia (straight arrow) and hyperchromtic nuclei in group3 (curved arrow). (H&E stain, ×200)
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Figure 7: Photomicrograph showing basilar hyperplasia (straight arrow) and hyperchromtic nuclei in group3 (curved arrow). (H&E stain, ×200)

Mentions: Labeling index determination and corresponding atypical morphological features were noted in the preceding H and E sections (group 3). The most common atypical morphological feature as seen in the H and E sections of dysplasia correlating with Cyclin D1 expression was basilar hyperplasia [Figures 7 and 8]. Of the 13 atypical morphologic features studied, Laplace estimate for basilar hyperplasia was 76.7% followed by nuclear hyperchromatism in 53.3% [Figures 7], prominent nucleoli in 43.3%, increased nuclear cytoplasmic ratio in 13.3%, and cellular pleomorphism in 10.0%.


Cyclin D1 an early biomarker in oral carcinogenesis.

Ramakrishna A, Shreedhar B, Narayan T, Mohanty L, Shenoy S, Jamadar S - J Oral Maxillofac Pathol (2013)

Photomicrograph showing basilar hyperplasia (straight arrow) and hyperchromtic nuclei in group3 (curved arrow). (H&E stain, ×200)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927334&req=5

Figure 7: Photomicrograph showing basilar hyperplasia (straight arrow) and hyperchromtic nuclei in group3 (curved arrow). (H&E stain, ×200)
Mentions: Labeling index determination and corresponding atypical morphological features were noted in the preceding H and E sections (group 3). The most common atypical morphological feature as seen in the H and E sections of dysplasia correlating with Cyclin D1 expression was basilar hyperplasia [Figures 7 and 8]. Of the 13 atypical morphologic features studied, Laplace estimate for basilar hyperplasia was 76.7% followed by nuclear hyperchromatism in 53.3% [Figures 7], prominent nucleoli in 43.3%, increased nuclear cytoplasmic ratio in 13.3%, and cellular pleomorphism in 10.0%.

Bottom Line: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant.Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, College of Dental Sciences, Davangere, Karnataka, India.

ABSTRACT

Background: Dysregulation of cell cycle is a fundamental hallmark of cancer progression. Cyclin D1, part of complex molecular system regulating G1-S point of cell cycle is overexpressed in variety of tumors.

Aims: To look for its immunohistochemical expression in clinically normal mucosa from patients with and without tobacco habits, leukoplakia; and correlate its expression to individual atypical morphologic features, as seen in hematoxylin and eosin (H and E) sections of leukoplakia exhibiting dysplasia.

Materials and methods: We examined the expression of cyclin D1 in immunohistochemically stained sections of 15 normal buccal mucosa without any habits (group 1), 30 clinically normal mucosa from tobacco habituιs (group 2) and 30 leukoplakias exhibiting dysplasias (group 3). Descriptive statistical analysis performed. Results presented on Mean ± Standard deviation and in number (%). Adjusted Wald 95% Confidence Interval (CI) computed, percentages of morphological features assessed by Laplace estimate. Mann-Whitney U, Kruskal-Wallis test used to find the percentage expression of cyclin D1.

Results: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant. Generally expression of cyclin D1 was confined to lower one-third of epithelium and was highest in mild dysplasias. Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.

Conclusion: The altered pattern of cyclin D1 expression here may be an early event in conversion of normal epithelium into dysplastic epithelium and may serve as a biomarker of oral carcinogenesis. Its expression may be increased in tobacco habitués. Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

No MeSH data available.


Related in: MedlinePlus