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Cyclin D1 an early biomarker in oral carcinogenesis.

Ramakrishna A, Shreedhar B, Narayan T, Mohanty L, Shenoy S, Jamadar S - J Oral Maxillofac Pathol (2013)

Bottom Line: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant.Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, College of Dental Sciences, Davangere, Karnataka, India.

ABSTRACT

Background: Dysregulation of cell cycle is a fundamental hallmark of cancer progression. Cyclin D1, part of complex molecular system regulating G1-S point of cell cycle is overexpressed in variety of tumors.

Aims: To look for its immunohistochemical expression in clinically normal mucosa from patients with and without tobacco habits, leukoplakia; and correlate its expression to individual atypical morphologic features, as seen in hematoxylin and eosin (H and E) sections of leukoplakia exhibiting dysplasia.

Materials and methods: We examined the expression of cyclin D1 in immunohistochemically stained sections of 15 normal buccal mucosa without any habits (group 1), 30 clinically normal mucosa from tobacco habituιs (group 2) and 30 leukoplakias exhibiting dysplasias (group 3). Descriptive statistical analysis performed. Results presented on Mean ± Standard deviation and in number (%). Adjusted Wald 95% Confidence Interval (CI) computed, percentages of morphological features assessed by Laplace estimate. Mann-Whitney U, Kruskal-Wallis test used to find the percentage expression of cyclin D1.

Results: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant. Generally expression of cyclin D1 was confined to lower one-third of epithelium and was highest in mild dysplasias. Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.

Conclusion: The altered pattern of cyclin D1 expression here may be an early event in conversion of normal epithelium into dysplastic epithelium and may serve as a biomarker of oral carcinogenesis. Its expression may be increased in tobacco habitués. Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

No MeSH data available.


Related in: MedlinePlus

Sections showing normal epithelium and connective tissue (group 1). (H&E stain, ×200)
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Figure 1: Sections showing normal epithelium and connective tissue (group 1). (H&E stain, ×200)

Mentions: Detectable cyclin D1 expression, light-dark brown staining, confined to cell nuclei was found in basal and parabasal layers of the epithelium in 12 of 15 cases of clinically normal mucosa without tobacco smoking habit (group 1). The expression was confined mainly to parabasal layer with only few cells in the basal layer showing positivity [Figures 1 and 2]. The mean LI determined was 3.48% [Table 3].


Cyclin D1 an early biomarker in oral carcinogenesis.

Ramakrishna A, Shreedhar B, Narayan T, Mohanty L, Shenoy S, Jamadar S - J Oral Maxillofac Pathol (2013)

Sections showing normal epithelium and connective tissue (group 1). (H&E stain, ×200)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927334&req=5

Figure 1: Sections showing normal epithelium and connective tissue (group 1). (H&E stain, ×200)
Mentions: Detectable cyclin D1 expression, light-dark brown staining, confined to cell nuclei was found in basal and parabasal layers of the epithelium in 12 of 15 cases of clinically normal mucosa without tobacco smoking habit (group 1). The expression was confined mainly to parabasal layer with only few cells in the basal layer showing positivity [Figures 1 and 2]. The mean LI determined was 3.48% [Table 3].

Bottom Line: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant.Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, College of Dental Sciences, Davangere, Karnataka, India.

ABSTRACT

Background: Dysregulation of cell cycle is a fundamental hallmark of cancer progression. Cyclin D1, part of complex molecular system regulating G1-S point of cell cycle is overexpressed in variety of tumors.

Aims: To look for its immunohistochemical expression in clinically normal mucosa from patients with and without tobacco habits, leukoplakia; and correlate its expression to individual atypical morphologic features, as seen in hematoxylin and eosin (H and E) sections of leukoplakia exhibiting dysplasia.

Materials and methods: We examined the expression of cyclin D1 in immunohistochemically stained sections of 15 normal buccal mucosa without any habits (group 1), 30 clinically normal mucosa from tobacco habituιs (group 2) and 30 leukoplakias exhibiting dysplasias (group 3). Descriptive statistical analysis performed. Results presented on Mean ± Standard deviation and in number (%). Adjusted Wald 95% Confidence Interval (CI) computed, percentages of morphological features assessed by Laplace estimate. Mann-Whitney U, Kruskal-Wallis test used to find the percentage expression of cyclin D1.

Results: Expression of cyclin D1 in group 3 was significantly higher than in group 1 and 2 (P < 0.001, P = 0.028), expression in group 2 was significantly higher than in group 1 (P = 0.003) and were statistically significant. Generally expression of cyclin D1 was confined to lower one-third of epithelium and was highest in mild dysplasias. Among 13 atypical morphologic features, cyclin D1 expression consistently correlated with basilar hyperplasia.

Conclusion: The altered pattern of cyclin D1 expression here may be an early event in conversion of normal epithelium into dysplastic epithelium and may serve as a biomarker of oral carcinogenesis. Its expression may be increased in tobacco habitués. Basilar hyperplasia should be given additional weightage in the grading system in predicting the fate of affected epithelium.

No MeSH data available.


Related in: MedlinePlus