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Histomorphometric analysis of vascularity in normal buccal mucosa, leukoplakia, and squamous cell carcinoma of buccal mucosa.

Pujari RK, Vanaki SS, Puranik RS, Desai RS, Motupalli N, Halawar S - J Oral Maxillofac Pathol (2013)

Bottom Line: The difference of MVD between H and E and MT was statistically significant.It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).Further studies are needed to standardize baseline levels for different sites and age groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Rajarajeshwari Dental College, Bangalore, India.

ABSTRACT

Context: Angiogenesis, the process that leads to the formation of new blood vessels, continues to be a topic of major scientific interest. There is an increasing hope that new discoveries will lead to newer therapies that target angiogenesis as a reliable option for disease therapy.

Aims: The objective of this study was to assess the role of vascularity, correlation of morphometric aspects of vascularity, investigate its usefulness in the histopathological classification and prognosis in normal buccal mucosa (NBM), leukoplakia and squamous cell carcinoma (SCC) of buccal mucosa.

Materials and methods: The study sample consisted of 15 cases of NBM, 30 cases of leukoplakia, and 30 cases of SCC of buccal mucosa. The 75 archival samples were stained by hemotoxylin and eosin (H and E) and Masson's trichrome (MT). The stained sections were analyzed using image analysis software.

Statistical analysis used: Statistical Package for Social Sciences (SPSS) 12.0 statistical software.

Results: The combined mean vessel density (MVD) of all the cases in H and E was 0.1112 and for MT it was 0.2150. The difference of MVD between H and E and MT was statistically significant. The mean MVD in SCC (0.3455) for MT was higher than NBM (0.1314) and leukoplakia (0.1263). The mean MVD increased from stage III (0.3563) to IV (0.5312). It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).

Conclusions: MVD can be used as an adjunct with other diagnostic modalities. Further studies are needed to standardize baseline levels for different sites and age groups.

No MeSH data available.


Related in: MedlinePlus

Distribution of study subjects by group and gender
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Figure 2: Distribution of study subjects by group and gender

Mentions: A total number of 75 cases were taken for the study and the mean MVD of all the cases in H and E was 0.1112 and for MT 0.2150 with a P value of 0.0017, which was statistically significant [Table 1]. The percentage of distribution of study subjects by three groups and gender in normal revealed 33.33 and 66.67% for female and male, respectively. In leukoplakia 3.33% for females and 96.67% for males and in SCC 46.67% are males [Graph 1]. The mean age of normal, leukoplakia, and SCC is 35, 43, and 51 years, respectively [Graph 2]. The mean MVD in 15 controls for H and E is 0.0611 and for MT 0.1314 with P value of 0.2823. Though the MVD was higher in MT but it was not significant statistically. The MVD in leukoplakia of buccal mucosa (30 cases) and SCC of buccal mucosa (30 cases) for H and E and MT groups were compared. The MVD for H and E was 0.0352 and for MT 0.1263 in leukoplakia of buccal mucosa with a P value of 0.0000 which is statistically significant. The MVD for H and E and MT groups in SCC of buccal mucosa was 0.2122 and 0.3455, respectively with a P value of 0.0413 which was statistically significant [Table 2].


Histomorphometric analysis of vascularity in normal buccal mucosa, leukoplakia, and squamous cell carcinoma of buccal mucosa.

Pujari RK, Vanaki SS, Puranik RS, Desai RS, Motupalli N, Halawar S - J Oral Maxillofac Pathol (2013)

Distribution of study subjects by group and gender
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927331&req=5

Figure 2: Distribution of study subjects by group and gender
Mentions: A total number of 75 cases were taken for the study and the mean MVD of all the cases in H and E was 0.1112 and for MT 0.2150 with a P value of 0.0017, which was statistically significant [Table 1]. The percentage of distribution of study subjects by three groups and gender in normal revealed 33.33 and 66.67% for female and male, respectively. In leukoplakia 3.33% for females and 96.67% for males and in SCC 46.67% are males [Graph 1]. The mean age of normal, leukoplakia, and SCC is 35, 43, and 51 years, respectively [Graph 2]. The mean MVD in 15 controls for H and E is 0.0611 and for MT 0.1314 with P value of 0.2823. Though the MVD was higher in MT but it was not significant statistically. The MVD in leukoplakia of buccal mucosa (30 cases) and SCC of buccal mucosa (30 cases) for H and E and MT groups were compared. The MVD for H and E was 0.0352 and for MT 0.1263 in leukoplakia of buccal mucosa with a P value of 0.0000 which is statistically significant. The MVD for H and E and MT groups in SCC of buccal mucosa was 0.2122 and 0.3455, respectively with a P value of 0.0413 which was statistically significant [Table 2].

Bottom Line: The difference of MVD between H and E and MT was statistically significant.It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).Further studies are needed to standardize baseline levels for different sites and age groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Rajarajeshwari Dental College, Bangalore, India.

ABSTRACT

Context: Angiogenesis, the process that leads to the formation of new blood vessels, continues to be a topic of major scientific interest. There is an increasing hope that new discoveries will lead to newer therapies that target angiogenesis as a reliable option for disease therapy.

Aims: The objective of this study was to assess the role of vascularity, correlation of morphometric aspects of vascularity, investigate its usefulness in the histopathological classification and prognosis in normal buccal mucosa (NBM), leukoplakia and squamous cell carcinoma (SCC) of buccal mucosa.

Materials and methods: The study sample consisted of 15 cases of NBM, 30 cases of leukoplakia, and 30 cases of SCC of buccal mucosa. The 75 archival samples were stained by hemotoxylin and eosin (H and E) and Masson's trichrome (MT). The stained sections were analyzed using image analysis software.

Statistical analysis used: Statistical Package for Social Sciences (SPSS) 12.0 statistical software.

Results: The combined mean vessel density (MVD) of all the cases in H and E was 0.1112 and for MT it was 0.2150. The difference of MVD between H and E and MT was statistically significant. The mean MVD in SCC (0.3455) for MT was higher than NBM (0.1314) and leukoplakia (0.1263). The mean MVD increased from stage III (0.3563) to IV (0.5312). It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).

Conclusions: MVD can be used as an adjunct with other diagnostic modalities. Further studies are needed to standardize baseline levels for different sites and age groups.

No MeSH data available.


Related in: MedlinePlus