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Histomorphometric analysis of vascularity in normal buccal mucosa, leukoplakia, and squamous cell carcinoma of buccal mucosa.

Pujari RK, Vanaki SS, Puranik RS, Desai RS, Motupalli N, Halawar S - J Oral Maxillofac Pathol (2013)

Bottom Line: The difference of MVD between H and E and MT was statistically significant.It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).Further studies are needed to standardize baseline levels for different sites and age groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Rajarajeshwari Dental College, Bangalore, India.

ABSTRACT

Context: Angiogenesis, the process that leads to the formation of new blood vessels, continues to be a topic of major scientific interest. There is an increasing hope that new discoveries will lead to newer therapies that target angiogenesis as a reliable option for disease therapy.

Aims: The objective of this study was to assess the role of vascularity, correlation of morphometric aspects of vascularity, investigate its usefulness in the histopathological classification and prognosis in normal buccal mucosa (NBM), leukoplakia and squamous cell carcinoma (SCC) of buccal mucosa.

Materials and methods: The study sample consisted of 15 cases of NBM, 30 cases of leukoplakia, and 30 cases of SCC of buccal mucosa. The 75 archival samples were stained by hemotoxylin and eosin (H and E) and Masson's trichrome (MT). The stained sections were analyzed using image analysis software.

Statistical analysis used: Statistical Package for Social Sciences (SPSS) 12.0 statistical software.

Results: The combined mean vessel density (MVD) of all the cases in H and E was 0.1112 and for MT it was 0.2150. The difference of MVD between H and E and MT was statistically significant. The mean MVD in SCC (0.3455) for MT was higher than NBM (0.1314) and leukoplakia (0.1263). The mean MVD increased from stage III (0.3563) to IV (0.5312). It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).

Conclusions: MVD can be used as an adjunct with other diagnostic modalities. Further studies are needed to standardize baseline levels for different sites and age groups.

No MeSH data available.


Related in: MedlinePlus

(1a) Photomicrograph of normal buccal mucosa (H&E stain, ×400), (1b) Photomicrograph of normal buccal mucosa (Masson's trichrome stain, ×400), (1c) Normal buccal mucosal image analyzed by morphometry (Masson's trichrome stain, ×400). (2a) Photomicrograph of leukoplakia sections (H&E stain, ×400), (2b) Photomicrograph of leukoplakia sections (Masson's trichrome, ×400), (2c) Image of Leukoplakia analyzed by morphometry. (Masson's trichrome, ×400). (3a) Photomicrograph of moderately differentiated squamous cell carcinoma (H&E stain, ×400), (3b) Photomicrograh of moderately differentiated squamous cell carcinoma (Masson's trichrome stain, ×400), (3c) Image of moderately differentiated squamous cell carcinoma analyzed by morphometry (Masson's trichrome stain, ×400)
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Figure 1: (1a) Photomicrograph of normal buccal mucosa (H&E stain, ×400), (1b) Photomicrograph of normal buccal mucosa (Masson's trichrome stain, ×400), (1c) Normal buccal mucosal image analyzed by morphometry (Masson's trichrome stain, ×400). (2a) Photomicrograph of leukoplakia sections (H&E stain, ×400), (2b) Photomicrograph of leukoplakia sections (Masson's trichrome, ×400), (2c) Image of Leukoplakia analyzed by morphometry. (Masson's trichrome, ×400). (3a) Photomicrograph of moderately differentiated squamous cell carcinoma (H&E stain, ×400), (3b) Photomicrograh of moderately differentiated squamous cell carcinoma (Masson's trichrome stain, ×400), (3c) Image of moderately differentiated squamous cell carcinoma analyzed by morphometry (Masson's trichrome stain, ×400)

Mentions: MVD: The ratio of vascular tissue area to total tissue area in the digital images imported to image analyzer software[1] [Figure 1].


Histomorphometric analysis of vascularity in normal buccal mucosa, leukoplakia, and squamous cell carcinoma of buccal mucosa.

Pujari RK, Vanaki SS, Puranik RS, Desai RS, Motupalli N, Halawar S - J Oral Maxillofac Pathol (2013)

(1a) Photomicrograph of normal buccal mucosa (H&E stain, ×400), (1b) Photomicrograph of normal buccal mucosa (Masson's trichrome stain, ×400), (1c) Normal buccal mucosal image analyzed by morphometry (Masson's trichrome stain, ×400). (2a) Photomicrograph of leukoplakia sections (H&E stain, ×400), (2b) Photomicrograph of leukoplakia sections (Masson's trichrome, ×400), (2c) Image of Leukoplakia analyzed by morphometry. (Masson's trichrome, ×400). (3a) Photomicrograph of moderately differentiated squamous cell carcinoma (H&E stain, ×400), (3b) Photomicrograh of moderately differentiated squamous cell carcinoma (Masson's trichrome stain, ×400), (3c) Image of moderately differentiated squamous cell carcinoma analyzed by morphometry (Masson's trichrome stain, ×400)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927331&req=5

Figure 1: (1a) Photomicrograph of normal buccal mucosa (H&E stain, ×400), (1b) Photomicrograph of normal buccal mucosa (Masson's trichrome stain, ×400), (1c) Normal buccal mucosal image analyzed by morphometry (Masson's trichrome stain, ×400). (2a) Photomicrograph of leukoplakia sections (H&E stain, ×400), (2b) Photomicrograph of leukoplakia sections (Masson's trichrome, ×400), (2c) Image of Leukoplakia analyzed by morphometry. (Masson's trichrome, ×400). (3a) Photomicrograph of moderately differentiated squamous cell carcinoma (H&E stain, ×400), (3b) Photomicrograh of moderately differentiated squamous cell carcinoma (Masson's trichrome stain, ×400), (3c) Image of moderately differentiated squamous cell carcinoma analyzed by morphometry (Masson's trichrome stain, ×400)
Mentions: MVD: The ratio of vascular tissue area to total tissue area in the digital images imported to image analyzer software[1] [Figure 1].

Bottom Line: The difference of MVD between H and E and MT was statistically significant.It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).Further studies are needed to standardize baseline levels for different sites and age groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Rajarajeshwari Dental College, Bangalore, India.

ABSTRACT

Context: Angiogenesis, the process that leads to the formation of new blood vessels, continues to be a topic of major scientific interest. There is an increasing hope that new discoveries will lead to newer therapies that target angiogenesis as a reliable option for disease therapy.

Aims: The objective of this study was to assess the role of vascularity, correlation of morphometric aspects of vascularity, investigate its usefulness in the histopathological classification and prognosis in normal buccal mucosa (NBM), leukoplakia and squamous cell carcinoma (SCC) of buccal mucosa.

Materials and methods: The study sample consisted of 15 cases of NBM, 30 cases of leukoplakia, and 30 cases of SCC of buccal mucosa. The 75 archival samples were stained by hemotoxylin and eosin (H and E) and Masson's trichrome (MT). The stained sections were analyzed using image analysis software.

Statistical analysis used: Statistical Package for Social Sciences (SPSS) 12.0 statistical software.

Results: The combined mean vessel density (MVD) of all the cases in H and E was 0.1112 and for MT it was 0.2150. The difference of MVD between H and E and MT was statistically significant. The mean MVD in SCC (0.3455) for MT was higher than NBM (0.1314) and leukoplakia (0.1263). The mean MVD increased from stage III (0.3563) to IV (0.5312). It also increased from NBM (0.1314) to hyperkeratosis (0.1505) and decreased from grade I (0.3556) to II (0.2795) of oral SCC (OSCC).

Conclusions: MVD can be used as an adjunct with other diagnostic modalities. Further studies are needed to standardize baseline levels for different sites and age groups.

No MeSH data available.


Related in: MedlinePlus