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Proliferating trichilemmal cyst: the value of ki67 immunostaining.

Rangel-Gamboa L, Reyes-Castro M, Dominguez-Cherit J, Vega-Memije E - Int J Trichology (2013)

Bottom Line: In 12 cases, ki67 immunostaining was carried out.Clinical follow-up was done and revealed that no patient had local recurrence in 5 years after surgical removal of PTC.We therefore consider this immunostaining technique is probably correlated with low recurrence potential.

View Article: PubMed Central - PubMed

Affiliation: Division of Investigation, General Hospital "Dr. Manuel Gea González", Calz. Tlalpan 4800, Seccion VX, C.P 14000, México.

ABSTRACT

Background: A proliferating trichilemmal cyst (PTC) is an uncommon, rapidly-reproducing cutaneous epithelial cyst, differentiating from the isthmic portion of the outer hair root sheath. It is usually described as a benign tumor, but malignant transformation has been reported and is then denominated as a malignant proliferating trichilemmal tumor. Ki67 immunostaining has been used as a methodology for the evaluation of tumor grade in other tumors, due to its distinctive reaction patterns which exclusively involve proliferating cells.

Objectives: (1) To report the incidence of cases of PTCs in a General Hospital during a 12 years period. (2) To determine the expression of ki67 using immunohistochemical staining. (3) To correlate ki67 reaction patterns with clinical prognosis.

Materials and methods: The dermatology department's files during a period of 12 years were reviewed; cases with a diagnosis of PTC were selected, and ki67 immunostaining was done when enough biological material was available.

Results: A total of 15 cases with a diagnosis of PTC were identified. In 12 cases, ki67 immunostaining was carried out. In 9 of the 12 cases, ki67 was observed in the basal cells of the cystic epithelium, one case was moderately positive in palisading epithelial cells; in the other two cases ki67 immunostaining was negative. Clinical follow-up was done and revealed that no patient had local recurrence in 5 years after surgical removal of PTC. We therefore consider this immunostaining technique is probably correlated with low recurrence potential.

No MeSH data available.


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Immunohistochemical staining with ki67 observed in the palisading epithelial basal cells of the cystic epithelium
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Figure 3: Immunohistochemical staining with ki67 observed in the palisading epithelial basal cells of the cystic epithelium

Mentions: Microscopic examination of all the tumors revealed variously shaped and sized lobules formed by aggregations of squamous epithelial cells and peripherally-palisading epithelial cells maturing into large pale-staining cells displaying an abrupt trichilemmal type of keratinization [Figure 2]. The mitotic activity and necrosis were variable, but all tumors belonged to group I in accord with the classification proposed by Ye et al.[16] None of the tumors presented infiltrative margins or metastasis. In 12 (80%) cases, ki67 immunostaining was performed. In nine of these cases, ki67 immunostaining was observed in the basal cells of the cystic epithelium [Figure 3], without evidence of a malignant proliferation pattern. One case was moderately positive in palisading epithelial cells. Finally in the other two cases, ki67 immunostaining was negative.


Proliferating trichilemmal cyst: the value of ki67 immunostaining.

Rangel-Gamboa L, Reyes-Castro M, Dominguez-Cherit J, Vega-Memije E - Int J Trichology (2013)

Immunohistochemical staining with ki67 observed in the palisading epithelial basal cells of the cystic epithelium
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3927166&req=5

Figure 3: Immunohistochemical staining with ki67 observed in the palisading epithelial basal cells of the cystic epithelium
Mentions: Microscopic examination of all the tumors revealed variously shaped and sized lobules formed by aggregations of squamous epithelial cells and peripherally-palisading epithelial cells maturing into large pale-staining cells displaying an abrupt trichilemmal type of keratinization [Figure 2]. The mitotic activity and necrosis were variable, but all tumors belonged to group I in accord with the classification proposed by Ye et al.[16] None of the tumors presented infiltrative margins or metastasis. In 12 (80%) cases, ki67 immunostaining was performed. In nine of these cases, ki67 immunostaining was observed in the basal cells of the cystic epithelium [Figure 3], without evidence of a malignant proliferation pattern. One case was moderately positive in palisading epithelial cells. Finally in the other two cases, ki67 immunostaining was negative.

Bottom Line: In 12 cases, ki67 immunostaining was carried out.Clinical follow-up was done and revealed that no patient had local recurrence in 5 years after surgical removal of PTC.We therefore consider this immunostaining technique is probably correlated with low recurrence potential.

View Article: PubMed Central - PubMed

Affiliation: Division of Investigation, General Hospital "Dr. Manuel Gea González", Calz. Tlalpan 4800, Seccion VX, C.P 14000, México.

ABSTRACT

Background: A proliferating trichilemmal cyst (PTC) is an uncommon, rapidly-reproducing cutaneous epithelial cyst, differentiating from the isthmic portion of the outer hair root sheath. It is usually described as a benign tumor, but malignant transformation has been reported and is then denominated as a malignant proliferating trichilemmal tumor. Ki67 immunostaining has been used as a methodology for the evaluation of tumor grade in other tumors, due to its distinctive reaction patterns which exclusively involve proliferating cells.

Objectives: (1) To report the incidence of cases of PTCs in a General Hospital during a 12 years period. (2) To determine the expression of ki67 using immunohistochemical staining. (3) To correlate ki67 reaction patterns with clinical prognosis.

Materials and methods: The dermatology department's files during a period of 12 years were reviewed; cases with a diagnosis of PTC were selected, and ki67 immunostaining was done when enough biological material was available.

Results: A total of 15 cases with a diagnosis of PTC were identified. In 12 cases, ki67 immunostaining was carried out. In 9 of the 12 cases, ki67 was observed in the basal cells of the cystic epithelium, one case was moderately positive in palisading epithelial cells; in the other two cases ki67 immunostaining was negative. Clinical follow-up was done and revealed that no patient had local recurrence in 5 years after surgical removal of PTC. We therefore consider this immunostaining technique is probably correlated with low recurrence potential.

No MeSH data available.


Related in: MedlinePlus