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Possible role of the α7 nicotinic receptors in mediating nicotine's effect on developing lung - implications in unexplained human perinatal death.

Lavezzi AM, Corna MF, Alfonsi G, Matturri L - BMC Pulm Med (2014)

Bottom Line: Prenatal nicotine absorption, in particular, is associated with alterations in lung development and functions at birth and with respiratory disorders in infancy.In addition, we observed a significantly higher incidence of strong α7 nAChR immunostaining in lung epithelial cells and lung vessel walls in sudden fetal and infant death cases with a smoker mother than in age-matched controls.This work stresses the importance of implementing preventable measures to decrease the noxious potential of nicotine in pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical, Surgical and Dental Sciences, "Lino Rossi" Research Center for the study and prevention of unexpected perinatal death and SIDS, University of Milan, Via della Commenda 19, 20122 Milan, Italy. anna.lavezzi@unimi.it.

ABSTRACT

Background: It is well known that maternal smoking during pregnancy is very harmful to the fetus. Prenatal nicotine absorption, in particular, is associated with alterations in lung development and functions at birth and with respiratory disorders in infancy. Many of the pulmonary disorders are mediated by the interaction of nicotine with the nicotinic receptors (nAChRs), above all with the α7 nAChR subunits that are widely expressed in the developing lung. To determine whether the lung hypoplasia frequently observed in victims of sudden fetal and neonatal death with a smoker mother may result from nicotine interacting with lung nicotinic receptors, we investigated by immunohistochemistry the possible presence of the α7 nAChR subunit overexpression in these pathologies.

Methods: In lung histological sections from 45 subjects who died of sudden intrauterine unexplained death syndrome (SIUDS) and 15 subjects who died of sudden infant death syndrome (SIDS), we applied the radial alveolar count (RAC) to evaluate the degree of lung maturation, and the immunohistochemical technique for nAChRs, in particular for the α7 nAChR subunit identification. In the same cases, an in-depth study of the autonomic nervous system was performed to highlight possible developmental alterations of the main vital centers located in the brainstem.

Results: We diagnosed a "lung hypoplasia", on the basis of RAC values lower than the normal reference values, in 63% of SIUDS/SIDS cases and 8% of controls. In addition, we observed a significantly higher incidence of strong α7 nAChR immunostaining in lung epithelial cells and lung vessel walls in sudden fetal and infant death cases with a smoker mother than in age-matched controls. Hypoplasia of the raphe, the parafacial, the Kölliker-Fuse, the arcuate and the pre-Bötzinger nuclei was at the same time present in the brainstem of these victims.

Conclusions: These findings demonstrate that when crossing the placenta, nicotine can interact with nicotinic receptors of both neuronal and non-neuronal cells, leading to lung and nervous system defective development, respectively. This work stresses the importance of implementing preventable measures to decrease the noxious potential of nicotine in pregnancy.

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Histological section of lung parenchima showing the radial alveolar count (RAC) method. SIDS case, 1 month-old (RAC: 2.5; normal mean value for age: 5.5 ± 1.4). Hematoxylin-eosin stain; magnification: 10×.
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Figure 1: Histological section of lung parenchima showing the radial alveolar count (RAC) method. SIDS case, 1 month-old (RAC: 2.5; normal mean value for age: 5.5 ± 1.4). Hematoxylin-eosin stain; magnification: 10×.

Mentions: In 38 cases (63%) of sudden death (33 SIUDS and 5 SIDS), a paucity of pulmonary alveoli was highlighted in histological sections demonstrating lower radial counts than the normal reference values (Figure 1). The mean RAC value was 2.1 ±0.4 in 12 fetuses aged 32–35 gws (normal value: 3.2 ± 0.9), and 2.7 ± 0.2 in 21 fetuses aged 36–39 gws (normal value: 3.6 ± 0.9), while the mean RAC value in the 5 newborns (aged from 1 to 3 postnatal months) was 3.0 ± 0.5 (normal value: 5.5 ± 1.4). These observations supported the diagnosis of “lung hypoplasia”.


Possible role of the α7 nicotinic receptors in mediating nicotine's effect on developing lung - implications in unexplained human perinatal death.

Lavezzi AM, Corna MF, Alfonsi G, Matturri L - BMC Pulm Med (2014)

Histological section of lung parenchima showing the radial alveolar count (RAC) method. SIDS case, 1 month-old (RAC: 2.5; normal mean value for age: 5.5 ± 1.4). Hematoxylin-eosin stain; magnification: 10×.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3926856&req=5

Figure 1: Histological section of lung parenchima showing the radial alveolar count (RAC) method. SIDS case, 1 month-old (RAC: 2.5; normal mean value for age: 5.5 ± 1.4). Hematoxylin-eosin stain; magnification: 10×.
Mentions: In 38 cases (63%) of sudden death (33 SIUDS and 5 SIDS), a paucity of pulmonary alveoli was highlighted in histological sections demonstrating lower radial counts than the normal reference values (Figure 1). The mean RAC value was 2.1 ±0.4 in 12 fetuses aged 32–35 gws (normal value: 3.2 ± 0.9), and 2.7 ± 0.2 in 21 fetuses aged 36–39 gws (normal value: 3.6 ± 0.9), while the mean RAC value in the 5 newborns (aged from 1 to 3 postnatal months) was 3.0 ± 0.5 (normal value: 5.5 ± 1.4). These observations supported the diagnosis of “lung hypoplasia”.

Bottom Line: Prenatal nicotine absorption, in particular, is associated with alterations in lung development and functions at birth and with respiratory disorders in infancy.In addition, we observed a significantly higher incidence of strong α7 nAChR immunostaining in lung epithelial cells and lung vessel walls in sudden fetal and infant death cases with a smoker mother than in age-matched controls.This work stresses the importance of implementing preventable measures to decrease the noxious potential of nicotine in pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical, Surgical and Dental Sciences, "Lino Rossi" Research Center for the study and prevention of unexpected perinatal death and SIDS, University of Milan, Via della Commenda 19, 20122 Milan, Italy. anna.lavezzi@unimi.it.

ABSTRACT

Background: It is well known that maternal smoking during pregnancy is very harmful to the fetus. Prenatal nicotine absorption, in particular, is associated with alterations in lung development and functions at birth and with respiratory disorders in infancy. Many of the pulmonary disorders are mediated by the interaction of nicotine with the nicotinic receptors (nAChRs), above all with the α7 nAChR subunits that are widely expressed in the developing lung. To determine whether the lung hypoplasia frequently observed in victims of sudden fetal and neonatal death with a smoker mother may result from nicotine interacting with lung nicotinic receptors, we investigated by immunohistochemistry the possible presence of the α7 nAChR subunit overexpression in these pathologies.

Methods: In lung histological sections from 45 subjects who died of sudden intrauterine unexplained death syndrome (SIUDS) and 15 subjects who died of sudden infant death syndrome (SIDS), we applied the radial alveolar count (RAC) to evaluate the degree of lung maturation, and the immunohistochemical technique for nAChRs, in particular for the α7 nAChR subunit identification. In the same cases, an in-depth study of the autonomic nervous system was performed to highlight possible developmental alterations of the main vital centers located in the brainstem.

Results: We diagnosed a "lung hypoplasia", on the basis of RAC values lower than the normal reference values, in 63% of SIUDS/SIDS cases and 8% of controls. In addition, we observed a significantly higher incidence of strong α7 nAChR immunostaining in lung epithelial cells and lung vessel walls in sudden fetal and infant death cases with a smoker mother than in age-matched controls. Hypoplasia of the raphe, the parafacial, the Kölliker-Fuse, the arcuate and the pre-Bötzinger nuclei was at the same time present in the brainstem of these victims.

Conclusions: These findings demonstrate that when crossing the placenta, nicotine can interact with nicotinic receptors of both neuronal and non-neuronal cells, leading to lung and nervous system defective development, respectively. This work stresses the importance of implementing preventable measures to decrease the noxious potential of nicotine in pregnancy.

Show MeSH
Related in: MedlinePlus