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An in vitro synergistic interaction of combinations of Thymus glabrescens essential oil and its main constituents with chloramphenicol.

Ilić BS, Kocić BD, Cirić VM, Ćvetković OG, Miladinović DL - ScientificWorldJournal (2014)

Bottom Line: The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol.A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects.The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Medicine, University of Niš, 18000 Niš, Serbia.

ABSTRACT
The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

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The derived isobolograms for the interaction of geraniol-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.
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fig2: The derived isobolograms for the interaction of geraniol-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.

Mentions: From all the tested combinations of geraniol-reference antibiotic (Figure 2), 26 (57.8%) showed synergism, 15 (33.3%) had an additive effect, and 4 (8.9%) had an antagonistic effect. Studies on E. coli ATCC 25922 and K. pneumoniae ATCC 700603 showed a synergistic pattern for seven ratios (FIC indices in the range 0.21–0.87). Synergy was also noted when tested against P. mirabilis ATCC 12453 and P. aeruginosa ATCC 27853 (six ratios, FIC indices in the range 0.43–0.87). Combinations with S. aureus ATCC 29213 indicated additive (five ratios) and antagonistic (four ratios) effects.


An in vitro synergistic interaction of combinations of Thymus glabrescens essential oil and its main constituents with chloramphenicol.

Ilić BS, Kocić BD, Cirić VM, Ćvetković OG, Miladinović DL - ScientificWorldJournal (2014)

The derived isobolograms for the interaction of geraniol-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926437&req=5

fig2: The derived isobolograms for the interaction of geraniol-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.
Mentions: From all the tested combinations of geraniol-reference antibiotic (Figure 2), 26 (57.8%) showed synergism, 15 (33.3%) had an additive effect, and 4 (8.9%) had an antagonistic effect. Studies on E. coli ATCC 25922 and K. pneumoniae ATCC 700603 showed a synergistic pattern for seven ratios (FIC indices in the range 0.21–0.87). Synergy was also noted when tested against P. mirabilis ATCC 12453 and P. aeruginosa ATCC 27853 (six ratios, FIC indices in the range 0.43–0.87). Combinations with S. aureus ATCC 29213 indicated additive (five ratios) and antagonistic (four ratios) effects.

Bottom Line: The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol.A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects.The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Medicine, University of Niš, 18000 Niš, Serbia.

ABSTRACT
The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

Show MeSH
Related in: MedlinePlus