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An in vitro synergistic interaction of combinations of Thymus glabrescens essential oil and its main constituents with chloramphenicol.

Ilić BS, Kocić BD, Cirić VM, Ćvetković OG, Miladinović DL - ScientificWorldJournal (2014)

Bottom Line: The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol.A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects.The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Medicine, University of Niš, 18000 Niš, Serbia.

ABSTRACT
The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

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Related in: MedlinePlus

The derived isobolograms for the interaction of T. glabrescens oil-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.
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Related In: Results  -  Collection


getmorefigures.php?uid=PMC3926437&req=5

fig1: The derived isobolograms for the interaction of T. glabrescens oil-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.

Mentions: The results of the possible interactions between the essential oil, geraniol, and thymol with the reference antibiotic are given in Figures 1–3.


An in vitro synergistic interaction of combinations of Thymus glabrescens essential oil and its main constituents with chloramphenicol.

Ilić BS, Kocić BD, Cirić VM, Ćvetković OG, Miladinović DL - ScientificWorldJournal (2014)

The derived isobolograms for the interaction of T. glabrescens oil-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926437&req=5

fig1: The derived isobolograms for the interaction of T. glabrescens oil-chloramphenicol and their treatment outcomes against the following: (a) E. coli ATCC 25922, (b) K. pneumoniae ATCC 700603, (c) P. mirabilis ATCC 12453, (d) P. aeruginosa ATCC 27853, and (e) S. aureus ATCC 29213.
Mentions: The results of the possible interactions between the essential oil, geraniol, and thymol with the reference antibiotic are given in Figures 1–3.

Bottom Line: The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol.A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects.The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Medicine, University of Niš, 18000 Niš, Serbia.

ABSTRACT
The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21-0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.

Show MeSH
Related in: MedlinePlus