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Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats.

Adaramoye OA, Akanni OO - Adv Pharmacol Sci (2014)

Bottom Line: Treatment with AA significantly reduced the relative weight of the organs and lipid parameters.In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced.Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan 20005, Nigeria.

ABSTRACT
We investigated the effects of methanol extract of Artocarpus altilis (AA) on atherogenic indices and redox status of cellular system of rats fed with dietary cholesterol while Questran (QUE) served as standard. Biochemical indices such as total cholesterol (TC), triglycerides (TG), low- and high-density lipoproteins-cholesterol (LDL-C and HDL-C), aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), reduced glutathione, glutathione-s-transferase, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) were assessed. Hypercholesterolemic (HC) rats had significantly increased relative weight of liver and heart. Dietary cholesterol caused a significant increase (P < 0.05) in the levels of serum, hepatic, and cardiac TC by 110%, 70%, and 85%, LDL-C by 79%, 82%, and 176%, and TG by 68%, 96%, and 62%, respectively. Treatment with AA significantly reduced the relative weight of the organs and lipid parameters. There were beneficial increases in serum and cardiac HDL-C levels in HC rats treated with AA. In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced. All biochemical and histological alterations were ameliorated upon treatment with AA. Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus

Changes in histology of liver samples of hypercholesterolemic rats treated with Artocarpus altilis and Questran for nine consecutive weeks (M ×400). HC: cholesterol at 30 mg/0.3 mL, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg. Black arrow shows portal congestion, periportal cellular infiltration, and vacuolar degeneration of hepatocytes.
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fig6: Changes in histology of liver samples of hypercholesterolemic rats treated with Artocarpus altilis and Questran for nine consecutive weeks (M ×400). HC: cholesterol at 30 mg/0.3 mL, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg. Black arrow shows portal congestion, periportal cellular infiltration, and vacuolar degeneration of hepatocytes.

Mentions: The histology of liver slide showed marked portal congestion, severe periportal cellular infiltration by mononuclear cells, and mild diffuse vacuolar degeneration of hepatocytes (Figure 6), while aorta from HC rats revealed large focal area of myofibril necrosis with severe hemorrhages and fibrous connective tissue laid down (Figure 7). Treatment with AA (200 mg/kg) reversed the adverse effect of high dietary cholesterol on the histological architecture of the aorta and liver of the rats. The histological results further corroborated the biochemical findings indicating the beneficial effects of AA in hypercholesterolemic rats.


Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats.

Adaramoye OA, Akanni OO - Adv Pharmacol Sci (2014)

Changes in histology of liver samples of hypercholesterolemic rats treated with Artocarpus altilis and Questran for nine consecutive weeks (M ×400). HC: cholesterol at 30 mg/0.3 mL, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg. Black arrow shows portal congestion, periportal cellular infiltration, and vacuolar degeneration of hepatocytes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3926424&req=5

fig6: Changes in histology of liver samples of hypercholesterolemic rats treated with Artocarpus altilis and Questran for nine consecutive weeks (M ×400). HC: cholesterol at 30 mg/0.3 mL, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg. Black arrow shows portal congestion, periportal cellular infiltration, and vacuolar degeneration of hepatocytes.
Mentions: The histology of liver slide showed marked portal congestion, severe periportal cellular infiltration by mononuclear cells, and mild diffuse vacuolar degeneration of hepatocytes (Figure 6), while aorta from HC rats revealed large focal area of myofibril necrosis with severe hemorrhages and fibrous connective tissue laid down (Figure 7). Treatment with AA (200 mg/kg) reversed the adverse effect of high dietary cholesterol on the histological architecture of the aorta and liver of the rats. The histological results further corroborated the biochemical findings indicating the beneficial effects of AA in hypercholesterolemic rats.

Bottom Line: Treatment with AA significantly reduced the relative weight of the organs and lipid parameters.In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced.Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan 20005, Nigeria.

ABSTRACT
We investigated the effects of methanol extract of Artocarpus altilis (AA) on atherogenic indices and redox status of cellular system of rats fed with dietary cholesterol while Questran (QUE) served as standard. Biochemical indices such as total cholesterol (TC), triglycerides (TG), low- and high-density lipoproteins-cholesterol (LDL-C and HDL-C), aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), reduced glutathione, glutathione-s-transferase, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) were assessed. Hypercholesterolemic (HC) rats had significantly increased relative weight of liver and heart. Dietary cholesterol caused a significant increase (P < 0.05) in the levels of serum, hepatic, and cardiac TC by 110%, 70%, and 85%, LDL-C by 79%, 82%, and 176%, and TG by 68%, 96%, and 62%, respectively. Treatment with AA significantly reduced the relative weight of the organs and lipid parameters. There were beneficial increases in serum and cardiac HDL-C levels in HC rats treated with AA. In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced. All biochemical and histological alterations were ameliorated upon treatment with AA. Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus