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Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats.

Adaramoye OA, Akanni OO - Adv Pharmacol Sci (2014)

Bottom Line: Treatment with AA significantly reduced the relative weight of the organs and lipid parameters.In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced.Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan 20005, Nigeria.

ABSTRACT
We investigated the effects of methanol extract of Artocarpus altilis (AA) on atherogenic indices and redox status of cellular system of rats fed with dietary cholesterol while Questran (QUE) served as standard. Biochemical indices such as total cholesterol (TC), triglycerides (TG), low- and high-density lipoproteins-cholesterol (LDL-C and HDL-C), aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), reduced glutathione, glutathione-s-transferase, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) were assessed. Hypercholesterolemic (HC) rats had significantly increased relative weight of liver and heart. Dietary cholesterol caused a significant increase (P < 0.05) in the levels of serum, hepatic, and cardiac TC by 110%, 70%, and 85%, LDL-C by 79%, 82%, and 176%, and TG by 68%, 96%, and 62%, respectively. Treatment with AA significantly reduced the relative weight of the organs and lipid parameters. There were beneficial increases in serum and cardiac HDL-C levels in HC rats treated with AA. In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced. All biochemical and histological alterations were ameliorated upon treatment with AA. Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus

Effects of methanol extract of Artocarpus artilis on the activities of hepatic and cardiac glutathione-s-transferase (GST) of hypercholesterolemic rats. *Significantly different from control (P < 0.05), **significantly different from HC (P < 0.05). HC: hypercholesterolemic rats, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg.
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fig5: Effects of methanol extract of Artocarpus artilis on the activities of hepatic and cardiac glutathione-s-transferase (GST) of hypercholesterolemic rats. *Significantly different from control (P < 0.05), **significantly different from HC (P < 0.05). HC: hypercholesterolemic rats, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg.

Mentions: Administration of dietary cholesterol significantly increased (P < 0.05) serum, hepatic, and cardiac lipid peroxidation (LPO) products measured as thiobarbituric acid reactive substances (TBARS) by 265%, 83%, and 80%, respectively (Table 3). However, treatment with AA completely ameliorated dietary cholesterol-induced increase in LPO. In HC rats, the activities of hepatic, and cardiac SOD and CAT as well as cardiac GPx decreased significantly relative to the control (Table 4). Specifically, hepatic SOD and CAT decreased by 54% and 45%, while cardiac SOD, CAT, and GPx decreased by 67%, 59%, and 36%, respectively. Also, activities of phase II and antioxidant enzyme (GST) in the liver of HC rats were significantly reduced when compared to controls (Figure 5). Administration of AA (200 mg/kg) reversed the adverse effect of high dietary cholesterol by normalizing these enzymic antioxidant indices. In HC rats, serum ALT, AST and LDH were significantly increased by 2.3-, 1.7-, and 2.4-fold, respectively, while cardiac LDH activity was decreased by 3.0-fold relative to controls (Table 5 and Figures 3 and 4). However, the observed elevations in the activities of these serum enzymes in HC rats were reversed following treatment with AA and quetsran.


Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats.

Adaramoye OA, Akanni OO - Adv Pharmacol Sci (2014)

Effects of methanol extract of Artocarpus artilis on the activities of hepatic and cardiac glutathione-s-transferase (GST) of hypercholesterolemic rats. *Significantly different from control (P < 0.05), **significantly different from HC (P < 0.05). HC: hypercholesterolemic rats, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3926424&req=5

fig5: Effects of methanol extract of Artocarpus artilis on the activities of hepatic and cardiac glutathione-s-transferase (GST) of hypercholesterolemic rats. *Significantly different from control (P < 0.05), **significantly different from HC (P < 0.05). HC: hypercholesterolemic rats, AA1: Artocarpus altilis at 100 mg/kg, AA2: Artocarpus altilis at 200 mg/kg, and QUE: Questran at 0.26 g/kg.
Mentions: Administration of dietary cholesterol significantly increased (P < 0.05) serum, hepatic, and cardiac lipid peroxidation (LPO) products measured as thiobarbituric acid reactive substances (TBARS) by 265%, 83%, and 80%, respectively (Table 3). However, treatment with AA completely ameliorated dietary cholesterol-induced increase in LPO. In HC rats, the activities of hepatic, and cardiac SOD and CAT as well as cardiac GPx decreased significantly relative to the control (Table 4). Specifically, hepatic SOD and CAT decreased by 54% and 45%, while cardiac SOD, CAT, and GPx decreased by 67%, 59%, and 36%, respectively. Also, activities of phase II and antioxidant enzyme (GST) in the liver of HC rats were significantly reduced when compared to controls (Figure 5). Administration of AA (200 mg/kg) reversed the adverse effect of high dietary cholesterol by normalizing these enzymic antioxidant indices. In HC rats, serum ALT, AST and LDH were significantly increased by 2.3-, 1.7-, and 2.4-fold, respectively, while cardiac LDH activity was decreased by 3.0-fold relative to controls (Table 5 and Figures 3 and 4). However, the observed elevations in the activities of these serum enzymes in HC rats were reversed following treatment with AA and quetsran.

Bottom Line: Treatment with AA significantly reduced the relative weight of the organs and lipid parameters.In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced.Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan 20005, Nigeria.

ABSTRACT
We investigated the effects of methanol extract of Artocarpus altilis (AA) on atherogenic indices and redox status of cellular system of rats fed with dietary cholesterol while Questran (QUE) served as standard. Biochemical indices such as total cholesterol (TC), triglycerides (TG), low- and high-density lipoproteins-cholesterol (LDL-C and HDL-C), aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), reduced glutathione, glutathione-s-transferase, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) were assessed. Hypercholesterolemic (HC) rats had significantly increased relative weight of liver and heart. Dietary cholesterol caused a significant increase (P < 0.05) in the levels of serum, hepatic, and cardiac TC by 110%, 70%, and 85%, LDL-C by 79%, 82%, and 176%, and TG by 68%, 96%, and 62%, respectively. Treatment with AA significantly reduced the relative weight of the organs and lipid parameters. There were beneficial increases in serum and cardiac HDL-C levels in HC rats treated with AA. In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced. All biochemical and histological alterations were ameliorated upon treatment with AA. Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus