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Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae.

Chumnanpuen P, Hansen MA, Smedsgaard J, Nielsen J - Int J Genomics (2014)

Bottom Line: Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae BY4709 (wild type) cultured on 3 different C-sources (glucose, glycerol, and ethanol) and sampled along 10 time points with 5 biological replicates.Both positive and negative electrospray ionization (ESI) modes were performed to obtain the complete information about the metabolite content.From the list of significant metabolite pairs, we reconstructed an interaction map that provides information of how different metabolic pathways have correlated patterns during growth on the different carbon sources.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

ABSTRACT
Metabolic footprinting offers a relatively easy approach to exploit the potentials of metabolomics for phenotypic characterization of microbial cells. To capture the highly dynamic nature of metabolites, we propose the use of dynamic metabolic footprinting instead of the traditional method which relies on analysis at a single time point. Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae BY4709 (wild type) cultured on 3 different C-sources (glucose, glycerol, and ethanol) and sampled along 10 time points with 5 biological replicates. In order to analyze the dynamic mass spectrometry data, we developed the novel analysis methods that allow us to perform correlation analysis to identify metabolites that significantly correlate over time during growth on the different carbon sources. Both positive and negative electrospray ionization (ESI) modes were performed to obtain the complete information about the metabolite content. Using sparse principal component analysis (Sparse PCA), we further identified those pairs of metabolites that significantly contribute to the separation. From the list of significant metabolite pairs, we reconstructed an interaction map that provides information of how different metabolic pathways have correlated patterns during growth on the different carbon sources.

No MeSH data available.


Related in: MedlinePlus

Standard PCA and loading show the clustering of different C-source cultures (including both ESI modes) and 5 replicates based on yield/slope of metabolite profile.
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fig4: Standard PCA and loading show the clustering of different C-source cultures (including both ESI modes) and 5 replicates based on yield/slope of metabolite profile.

Mentions: The upper triangular part of the matrix of the yields was extracted and unwrapped. Based on the matrix columns as shown with the cherry-picking method (Figure 2), we calculated the PCA loading for PCA analysis. Figure 4 illustrates the scores and loadings for the first two principal components using ordinary PCA. As can be seen from the plots, the yields of the selected metabolite pairs show a nice separation of the carbon sources. Still, when looking at the loadings we see that all metabolite pairs are assigned weights.


Dynamic Metabolic Footprinting Reveals the Key Components of Metabolic Network in Yeast Saccharomyces cerevisiae.

Chumnanpuen P, Hansen MA, Smedsgaard J, Nielsen J - Int J Genomics (2014)

Standard PCA and loading show the clustering of different C-source cultures (including both ESI modes) and 5 replicates based on yield/slope of metabolite profile.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926413&req=5

fig4: Standard PCA and loading show the clustering of different C-source cultures (including both ESI modes) and 5 replicates based on yield/slope of metabolite profile.
Mentions: The upper triangular part of the matrix of the yields was extracted and unwrapped. Based on the matrix columns as shown with the cherry-picking method (Figure 2), we calculated the PCA loading for PCA analysis. Figure 4 illustrates the scores and loadings for the first two principal components using ordinary PCA. As can be seen from the plots, the yields of the selected metabolite pairs show a nice separation of the carbon sources. Still, when looking at the loadings we see that all metabolite pairs are assigned weights.

Bottom Line: Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae BY4709 (wild type) cultured on 3 different C-sources (glucose, glycerol, and ethanol) and sampled along 10 time points with 5 biological replicates.Both positive and negative electrospray ionization (ESI) modes were performed to obtain the complete information about the metabolite content.From the list of significant metabolite pairs, we reconstructed an interaction map that provides information of how different metabolic pathways have correlated patterns during growth on the different carbon sources.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

ABSTRACT
Metabolic footprinting offers a relatively easy approach to exploit the potentials of metabolomics for phenotypic characterization of microbial cells. To capture the highly dynamic nature of metabolites, we propose the use of dynamic metabolic footprinting instead of the traditional method which relies on analysis at a single time point. Using direct infusion-mass spectrometry (DI-MS), we could observe the dynamic metabolic footprinting in yeast S. cerevisiae BY4709 (wild type) cultured on 3 different C-sources (glucose, glycerol, and ethanol) and sampled along 10 time points with 5 biological replicates. In order to analyze the dynamic mass spectrometry data, we developed the novel analysis methods that allow us to perform correlation analysis to identify metabolites that significantly correlate over time during growth on the different carbon sources. Both positive and negative electrospray ionization (ESI) modes were performed to obtain the complete information about the metabolite content. Using sparse principal component analysis (Sparse PCA), we further identified those pairs of metabolites that significantly contribute to the separation. From the list of significant metabolite pairs, we reconstructed an interaction map that provides information of how different metabolic pathways have correlated patterns during growth on the different carbon sources.

No MeSH data available.


Related in: MedlinePlus