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Fumigant antifungal activity of Corymbia citriodora and Cymbopogon nardus essential oils and citronellal against three fungal species.

Aguiar RW, Ootani MA, Ascencio SD, Ferreira TP, Dos Santos MM, dos Santos GR - ScientificWorldJournal (2014)

Bottom Line: The essential oils and citronellal were tested for their fumigant antifungal activity against Pyricularia (Magnaporthe) grisea, Aspergillus spp., and Colletotrichum musae.The contact assay using the essential oils and citronellal showed growth inhibition of the three fungal species.This showed that the crude component of essential oils of C. citriodora and C. nardus markedly suppressed spore production, germination, and growth inhibition of P. grisea, Aspergillus spp., and Colletotrichum musae.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal do Tocantins, Campus Universitário de Gurupi, 77410-530 Gurupi, TO, Brazil.

ABSTRACT
Corymbia citriodora and Cymbopogon nardus essential oils samples were analyzed by GC and GC-MS and their qualitative and quantitative compositions established. The main component of essential oils of C. citriodora and C. nardus was citronellal, at 61.78% and 36.6%, respectively. The essential oils and citronellal were tested for their fumigant antifungal activity against Pyricularia (Magnaporthe) grisea, Aspergillus spp., and Colletotrichum musae. The minimum inhibitory concentration (MIC) ranged from 100 to 200 ppm for the essential oils and 25 to 50 mg · mL(-1) for citronellal. The contact assay using the essential oils and citronellal showed growth inhibition of the three fungal species. However, a concentration of 1.47 mg · mL(-1) only reduced the inhibition of Aspergillus growth to 90% at 14 days of exposure. For the fumigant assay, 0.05, 0.11, and 0.23 mg · mL(-1) of essential oils and citronellal drastically affected growth of P. grisea, Aspergillus spp., and C. musae. Harmful effects on the sporulation and germination of the three fungi were seen, and there was complete inhibition at 0.15 mg · mL(-1) with both oils and citronellal. This showed that the crude component of essential oils of C. citriodora and C. nardus markedly suppressed spore production, germination, and growth inhibition of P. grisea, Aspergillus spp., and Colletotrichum musae.

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Related in: MedlinePlus

Effects of volatile compounds of C. nardus, C. citriodora, and the major constituent citronellal in the sporulation of P. grisea (a), Aspergillus spp. (b), and C. musae (c). Volatile effects of C. nardus, C. citriodora, and citronellal on spore germination (%) of P. grisea (d), Aspergillus spp. (e), and C. musae (f), 14 days after treatment.
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Related In: Results  -  Collection


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fig2: Effects of volatile compounds of C. nardus, C. citriodora, and the major constituent citronellal in the sporulation of P. grisea (a), Aspergillus spp. (b), and C. musae (c). Volatile effects of C. nardus, C. citriodora, and citronellal on spore germination (%) of P. grisea (d), Aspergillus spp. (e), and C. musae (f), 14 days after treatment.

Mentions: The spore production of P. grisea, Aspergillus spp., and C. musae was drastically affected by volatile activity of C. nardus and C. citriodora essential oils and citronellal, with a greater inhibition of spore production (Figures 2(a), 2(b), and 2(c)). The analyses revealed spore production of all fungi to be significantly affected (P < 0.05) by the oils, and this was inhibited completely at 0.15 mg·mL−1 (Figures 2(a), 2(b), and 2(c)). It is therefore likely that suppression of spore production by essential oil treatment could make a major contribution to limiting the spread of the pathogen by lowering the spore load in the storage atmosphere and on surfaces. This report describes the complex effect of essential oils from C. nardus and C. citriodora and citronellal on spore germination, with a result comparable to that obtained with other essential oils on different fungal species [26, 27].


Fumigant antifungal activity of Corymbia citriodora and Cymbopogon nardus essential oils and citronellal against three fungal species.

Aguiar RW, Ootani MA, Ascencio SD, Ferreira TP, Dos Santos MM, dos Santos GR - ScientificWorldJournal (2014)

Effects of volatile compounds of C. nardus, C. citriodora, and the major constituent citronellal in the sporulation of P. grisea (a), Aspergillus spp. (b), and C. musae (c). Volatile effects of C. nardus, C. citriodora, and citronellal on spore germination (%) of P. grisea (d), Aspergillus spp. (e), and C. musae (f), 14 days after treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926400&req=5

fig2: Effects of volatile compounds of C. nardus, C. citriodora, and the major constituent citronellal in the sporulation of P. grisea (a), Aspergillus spp. (b), and C. musae (c). Volatile effects of C. nardus, C. citriodora, and citronellal on spore germination (%) of P. grisea (d), Aspergillus spp. (e), and C. musae (f), 14 days after treatment.
Mentions: The spore production of P. grisea, Aspergillus spp., and C. musae was drastically affected by volatile activity of C. nardus and C. citriodora essential oils and citronellal, with a greater inhibition of spore production (Figures 2(a), 2(b), and 2(c)). The analyses revealed spore production of all fungi to be significantly affected (P < 0.05) by the oils, and this was inhibited completely at 0.15 mg·mL−1 (Figures 2(a), 2(b), and 2(c)). It is therefore likely that suppression of spore production by essential oil treatment could make a major contribution to limiting the spread of the pathogen by lowering the spore load in the storage atmosphere and on surfaces. This report describes the complex effect of essential oils from C. nardus and C. citriodora and citronellal on spore germination, with a result comparable to that obtained with other essential oils on different fungal species [26, 27].

Bottom Line: The essential oils and citronellal were tested for their fumigant antifungal activity against Pyricularia (Magnaporthe) grisea, Aspergillus spp., and Colletotrichum musae.The contact assay using the essential oils and citronellal showed growth inhibition of the three fungal species.This showed that the crude component of essential oils of C. citriodora and C. nardus markedly suppressed spore production, germination, and growth inhibition of P. grisea, Aspergillus spp., and Colletotrichum musae.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal do Tocantins, Campus Universitário de Gurupi, 77410-530 Gurupi, TO, Brazil.

ABSTRACT
Corymbia citriodora and Cymbopogon nardus essential oils samples were analyzed by GC and GC-MS and their qualitative and quantitative compositions established. The main component of essential oils of C. citriodora and C. nardus was citronellal, at 61.78% and 36.6%, respectively. The essential oils and citronellal were tested for their fumigant antifungal activity against Pyricularia (Magnaporthe) grisea, Aspergillus spp., and Colletotrichum musae. The minimum inhibitory concentration (MIC) ranged from 100 to 200 ppm for the essential oils and 25 to 50 mg · mL(-1) for citronellal. The contact assay using the essential oils and citronellal showed growth inhibition of the three fungal species. However, a concentration of 1.47 mg · mL(-1) only reduced the inhibition of Aspergillus growth to 90% at 14 days of exposure. For the fumigant assay, 0.05, 0.11, and 0.23 mg · mL(-1) of essential oils and citronellal drastically affected growth of P. grisea, Aspergillus spp., and C. musae. Harmful effects on the sporulation and germination of the three fungi were seen, and there was complete inhibition at 0.15 mg · mL(-1) with both oils and citronellal. This showed that the crude component of essential oils of C. citriodora and C. nardus markedly suppressed spore production, germination, and growth inhibition of P. grisea, Aspergillus spp., and Colletotrichum musae.

Show MeSH
Related in: MedlinePlus