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Residual HEMA and TEGDMA release and cytotoxicity evaluation of resin-modified glass ionomer cement and compomers cured with different light sources.

Botsali MS, Kuşgöz A, Altintaş SH, Ülker HE, Tanriver M, Kiliç S, Başak F, Ülker M - ScientificWorldJournal (2014)

Bottom Line: All material-LCU combinations decreased the fibroblast cells' viability more than the control group (P < 0.01), except for Dyract Extra cured with a halogen LCU (P > 0.05).Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for compomers.For Ketac N100, the halogen LCU decreased the cells' viability more than the LED LCU.

View Article: PubMed Central - PubMed

Affiliation: Department of Pedodontics, Faculty of Dentistry, Selcuk University, Selcuklu 42079, Konya, Turkey.

ABSTRACT
The purpose of this study was first to evaluate the elution of 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) monomers from resin-modified glass ionomer cement (RMGIC) and compomers cured with halogen and light-emitting diode (LED) light-curing units (LCUs). The effect of cured materials on the viability of L929 fibroblast cells was also evaluated. One RMGIC (Ketac N100) and two compomers (Dyract Extra and Twinkystar) were tested. Materials were prepared in teflon disks and light-cured with LED or halogen LCUs. The residual monomers of resin materials in solution were identified using high-performance liquid chromatography. The fibroblast cells' viability was analyzed using MTT assay. The type of LCU did not have a significant effect on the elution of HEMA and TEGDMA. A greater amount of HEMA than TEGMDA was eluted. The amount of TEGDMA eluted from Twinkystar was greater than Dyract Extra (P < 0.05) when cured with a halogen LCU. All material-LCU combinations decreased the fibroblast cells' viability more than the control group (P < 0.01), except for Dyract Extra cured with a halogen LCU (P > 0.05). Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for compomers. For Ketac N100, the halogen LCU decreased the cells' viability more than the LED LCU.

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Related in: MedlinePlus

Cytotoxicity of resin-modified glass inomer cement and compomers cured with halogen and LED light curing units. Data are expressed as percentage of the negative control cultures (n = 12).
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Related In: Results  -  Collection


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fig1: Cytotoxicity of resin-modified glass inomer cement and compomers cured with halogen and LED light curing units. Data are expressed as percentage of the negative control cultures (n = 12).

Mentions: The results of cytotoxicity testing are shown in Figure 1. The results show that, when cured with the LED LCU, all materials tested decreased the fibroblast cells' viability when compared with the control group (P < 0.01). All tested materials decreased cells' viability similarly (P > 0.05). When cured with the halogen LCU, the cell viability of Dyract Extra did not differ from that of the control group (P > 0.05), but Twinkystar and Ketac N100 decreased the cells' viability compared with control group (P < 0.01). Ketac N100 decreased the cells' viability more than Twinkystar, and Twinkystar decreased the cells' viability more than Dyract Extra (P < 0.05). Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for both compomers (Dyract Extra and Twinkystar); P = 0.000 and P = 0.008, respectively. For Ketac N 100, the halogen LCU decreased the cells' viability more than the LED LCU (P = 0.011).


Residual HEMA and TEGDMA release and cytotoxicity evaluation of resin-modified glass ionomer cement and compomers cured with different light sources.

Botsali MS, Kuşgöz A, Altintaş SH, Ülker HE, Tanriver M, Kiliç S, Başak F, Ülker M - ScientificWorldJournal (2014)

Cytotoxicity of resin-modified glass inomer cement and compomers cured with halogen and LED light curing units. Data are expressed as percentage of the negative control cultures (n = 12).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926398&req=5

fig1: Cytotoxicity of resin-modified glass inomer cement and compomers cured with halogen and LED light curing units. Data are expressed as percentage of the negative control cultures (n = 12).
Mentions: The results of cytotoxicity testing are shown in Figure 1. The results show that, when cured with the LED LCU, all materials tested decreased the fibroblast cells' viability when compared with the control group (P < 0.01). All tested materials decreased cells' viability similarly (P > 0.05). When cured with the halogen LCU, the cell viability of Dyract Extra did not differ from that of the control group (P > 0.05), but Twinkystar and Ketac N100 decreased the cells' viability compared with control group (P < 0.01). Ketac N100 decreased the cells' viability more than Twinkystar, and Twinkystar decreased the cells' viability more than Dyract Extra (P < 0.05). Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for both compomers (Dyract Extra and Twinkystar); P = 0.000 and P = 0.008, respectively. For Ketac N 100, the halogen LCU decreased the cells' viability more than the LED LCU (P = 0.011).

Bottom Line: All material-LCU combinations decreased the fibroblast cells' viability more than the control group (P < 0.01), except for Dyract Extra cured with a halogen LCU (P > 0.05).Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for compomers.For Ketac N100, the halogen LCU decreased the cells' viability more than the LED LCU.

View Article: PubMed Central - PubMed

Affiliation: Department of Pedodontics, Faculty of Dentistry, Selcuk University, Selcuklu 42079, Konya, Turkey.

ABSTRACT
The purpose of this study was first to evaluate the elution of 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) monomers from resin-modified glass ionomer cement (RMGIC) and compomers cured with halogen and light-emitting diode (LED) light-curing units (LCUs). The effect of cured materials on the viability of L929 fibroblast cells was also evaluated. One RMGIC (Ketac N100) and two compomers (Dyract Extra and Twinkystar) were tested. Materials were prepared in teflon disks and light-cured with LED or halogen LCUs. The residual monomers of resin materials in solution were identified using high-performance liquid chromatography. The fibroblast cells' viability was analyzed using MTT assay. The type of LCU did not have a significant effect on the elution of HEMA and TEGDMA. A greater amount of HEMA than TEGMDA was eluted. The amount of TEGDMA eluted from Twinkystar was greater than Dyract Extra (P < 0.05) when cured with a halogen LCU. All material-LCU combinations decreased the fibroblast cells' viability more than the control group (P < 0.01), except for Dyract Extra cured with a halogen LCU (P > 0.05). Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for compomers. For Ketac N100, the halogen LCU decreased the cells' viability more than the LED LCU.

Show MeSH
Related in: MedlinePlus