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Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Gonbad RA, Rani Sinniah U, Aziz MA, Mohamad R - ScientificWorldJournal (2014)

Bottom Line: The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃.TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300 Serdang, Malaysia ; Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

ABSTRACT
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA₃) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

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The Effect of dipping in different concentrations of IBA on percentage of rooted shoots.
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Related In: Results  -  Collection


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fig8: The Effect of dipping in different concentrations of IBA on percentage of rooted shoots.

Mentions: The result presented in Figure 8 clearly shows the importance of IBA for rooting of tea microshoots as no root formation was observed for treatment without IBA. Root initiation required about 40 days and another 20 days for development of a root system (Figure 9(a)) of the microshoots. A high percentage of rooting (72.3%) with development of healthy roots was observed by dipping the cut ends of the shoots in the solution of 300 mg/L IBA for 30 minutes and immediately transferred to half strength MS basal medium without any added growth regulators. Increasing the IBA concentration from 300 to 500 mg/L decreased the percentage of rooting (Figure 8). Well-developed plantlets were transferred to plastic pots (Figure 9(b)) with plastic cover for four weeks and then transferred to pot (Figure 9(c)). The final percentage of survival was 65% after 60 days in green house.


Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Gonbad RA, Rani Sinniah U, Aziz MA, Mohamad R - ScientificWorldJournal (2014)

The Effect of dipping in different concentrations of IBA on percentage of rooted shoots.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926387&req=5

fig8: The Effect of dipping in different concentrations of IBA on percentage of rooted shoots.
Mentions: The result presented in Figure 8 clearly shows the importance of IBA for rooting of tea microshoots as no root formation was observed for treatment without IBA. Root initiation required about 40 days and another 20 days for development of a root system (Figure 9(a)) of the microshoots. A high percentage of rooting (72.3%) with development of healthy roots was observed by dipping the cut ends of the shoots in the solution of 300 mg/L IBA for 30 minutes and immediately transferred to half strength MS basal medium without any added growth regulators. Increasing the IBA concentration from 300 to 500 mg/L decreased the percentage of rooting (Figure 8). Well-developed plantlets were transferred to plastic pots (Figure 9(b)) with plastic cover for four weeks and then transferred to pot (Figure 9(c)). The final percentage of survival was 65% after 60 days in green house.

Bottom Line: The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃.TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300 Serdang, Malaysia ; Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

ABSTRACT
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA₃) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

Show MeSH
Related in: MedlinePlus