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Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Gonbad RA, Rani Sinniah U, Aziz MA, Mohamad R - ScientificWorldJournal (2014)

Bottom Line: The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃.TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300 Serdang, Malaysia ; Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

ABSTRACT
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA₃) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

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Related in: MedlinePlus

Changes in fresh weight per explant basis as affected by different concentrations of BAP (mg/L) in combination with GA3 (mg/L) for tea clone Iran 100.
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Related In: Results  -  Collection


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fig4: Changes in fresh weight per explant basis as affected by different concentrations of BAP (mg/L) in combination with GA3 (mg/L) for tea clone Iran 100.

Mentions: To substantiate the data obtained on number of shoots, the fresh weight of explants produced was recorded. The results indicated highly significant differences (P ≤ 0.01) in fresh weight of explants among the different treatment combinations. In this study, the interaction between different concentrations of BAP in combination with GA3 was significant. The highest mean fresh weight per explant (380 mg) was obtained in MS media supplemented with 5 mg/L BAP in combination with 1 mg/L GA3 at the fourth subculture (Figure 4). As it can be seen in Figure 4, fresh weight increased with the increase in BAP concentration up to 5 mg/L due to the combined influence of increase in number of shoots as well as shoot height. At high concentration of BAP (7 mg/L) the explants favored callus formation, and this is the reason for the decrease in explant weight.


Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Gonbad RA, Rani Sinniah U, Aziz MA, Mohamad R - ScientificWorldJournal (2014)

Changes in fresh weight per explant basis as affected by different concentrations of BAP (mg/L) in combination with GA3 (mg/L) for tea clone Iran 100.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926387&req=5

fig4: Changes in fresh weight per explant basis as affected by different concentrations of BAP (mg/L) in combination with GA3 (mg/L) for tea clone Iran 100.
Mentions: To substantiate the data obtained on number of shoots, the fresh weight of explants produced was recorded. The results indicated highly significant differences (P ≤ 0.01) in fresh weight of explants among the different treatment combinations. In this study, the interaction between different concentrations of BAP in combination with GA3 was significant. The highest mean fresh weight per explant (380 mg) was obtained in MS media supplemented with 5 mg/L BAP in combination with 1 mg/L GA3 at the fourth subculture (Figure 4). As it can be seen in Figure 4, fresh weight increased with the increase in BAP concentration up to 5 mg/L due to the combined influence of increase in number of shoots as well as shoot height. At high concentration of BAP (7 mg/L) the explants favored callus formation, and this is the reason for the decrease in explant weight.

Bottom Line: The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃.TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300 Serdang, Malaysia ; Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

ABSTRACT
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA₃) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

Show MeSH
Related in: MedlinePlus