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Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Gonbad RA, Rani Sinniah U, Aziz MA, Mohamad R - ScientificWorldJournal (2014)

Bottom Line: The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃.TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300 Serdang, Malaysia ; Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

ABSTRACT
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA₃) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

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Related in: MedlinePlus

Effect of different concentrations of BAP (mg/L) on mean number of shoots per explant during shoot multiplication.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Effect of different concentrations of BAP (mg/L) on mean number of shoots per explant during shoot multiplication.

Mentions: The nodal segments showed multiplication after two weeks of culture in media supplemented with all concentrations of BAP. Although variations were observed in the response of explants to plant growth regulator treatment at the earlier subculture, the results reported here reflect the status of multiplication after four subcultures, reported on the performance per shoot basis and not as cumulative output after four subcultures. In this experiment, the simple effect of GA3 did not show any difference in the mean number of shoots produced (data not shown), but it was observed that shoot elongation occurred when GA3 was included in the media (Figure 2(b)). In contrast, the results revealed that the number of shoots produced was significantly (P ≤ 0.01) affected by the different concentration of BAP used and the highest mean number of shoots (6.8) was obtained on MS medium supplemented with 3 mg/L BAP (Figure 1). BAP was found to be necessary for multiplication as medium with 0 mg/L BAP did not show any multiplication except for growth of single shoot. Increased concentration of BAP increased the number of shoots produced attaining a maximum number at 3 mg/L BAP but further increase in BAP concentration (5 to 7 mg/L) reduced the number of shoots produced, showed necrosis, and had shoot fasciation. The reduced number of shoots could be due to inhibition of adventitious meristem elongation due to the use of higher BAP concentration as stated by Borchetia et al. [7]. The results of analysis of variance showed that there was no significant interaction between BAP and GA3 combinations. Despite the lack of interaction between BAP and GA3 on shoot multiplication, but based on plantlet morphology there were differences, which is shown in Figure 2. The control treatment devoid of BAP or GA3 had no multiplication or elongation (Figure 2(a)). While the inclusion of GA3 into BAP free medium resulted in elongation of single shoot (Figure 2(b)). The use of 1 mg/L BAP together with 0.5 mg/L GA3 stimulated the growth of axillary buds while 3 mg/L BAP in combination with 0.5 mg/L GA3 had multiple shoots with at least two shoots with a height of around 3 cm each containing about 5 to 6 nodal segments. In relation to effective multiplication, the presence of GA3 induced shoot elongation resulting in prominent nodal segments which can be utilized for further multiplication during the subculture.


Influence of cytokinins in combination with GA₃ on shoot multiplication and elongation of tea clone Iran 100 (Camellia sinensis (L.) O. Kuntze).

Gonbad RA, Rani Sinniah U, Aziz MA, Mohamad R - ScientificWorldJournal (2014)

Effect of different concentrations of BAP (mg/L) on mean number of shoots per explant during shoot multiplication.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3926387&req=5

fig1: Effect of different concentrations of BAP (mg/L) on mean number of shoots per explant during shoot multiplication.
Mentions: The nodal segments showed multiplication after two weeks of culture in media supplemented with all concentrations of BAP. Although variations were observed in the response of explants to plant growth regulator treatment at the earlier subculture, the results reported here reflect the status of multiplication after four subcultures, reported on the performance per shoot basis and not as cumulative output after four subcultures. In this experiment, the simple effect of GA3 did not show any difference in the mean number of shoots produced (data not shown), but it was observed that shoot elongation occurred when GA3 was included in the media (Figure 2(b)). In contrast, the results revealed that the number of shoots produced was significantly (P ≤ 0.01) affected by the different concentration of BAP used and the highest mean number of shoots (6.8) was obtained on MS medium supplemented with 3 mg/L BAP (Figure 1). BAP was found to be necessary for multiplication as medium with 0 mg/L BAP did not show any multiplication except for growth of single shoot. Increased concentration of BAP increased the number of shoots produced attaining a maximum number at 3 mg/L BAP but further increase in BAP concentration (5 to 7 mg/L) reduced the number of shoots produced, showed necrosis, and had shoot fasciation. The reduced number of shoots could be due to inhibition of adventitious meristem elongation due to the use of higher BAP concentration as stated by Borchetia et al. [7]. The results of analysis of variance showed that there was no significant interaction between BAP and GA3 combinations. Despite the lack of interaction between BAP and GA3 on shoot multiplication, but based on plantlet morphology there were differences, which is shown in Figure 2. The control treatment devoid of BAP or GA3 had no multiplication or elongation (Figure 2(a)). While the inclusion of GA3 into BAP free medium resulted in elongation of single shoot (Figure 2(b)). The use of 1 mg/L BAP together with 0.5 mg/L GA3 stimulated the growth of axillary buds while 3 mg/L BAP in combination with 0.5 mg/L GA3 had multiple shoots with at least two shoots with a height of around 3 cm each containing about 5 to 6 nodal segments. In relation to effective multiplication, the presence of GA3 induced shoot elongation resulting in prominent nodal segments which can be utilized for further multiplication during the subculture.

Bottom Line: The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃.TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L.Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300 Serdang, Malaysia ; Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

ABSTRACT
The use of in vitro culture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis (L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA₃) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA₃. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

Show MeSH
Related in: MedlinePlus