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Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet.

Dias FM, Leffa DD, Daumann F, Marques Sde O, Luciano TF, Possato JC, de Santana AA, Neves RX, Rosa JC, Oyama LM, Rodrigues B, de Andrade VM, de Souza CT, de Lira FS - Lipids Health Dis (2014)

Bottom Line: In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue.In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, 19060-900 Presidente Prudente, SP, Brazil. fabiolira@fct.unesp.br.

ABSTRACT

Background: Obesity has been studied as a metabolic and an inflammatory disease and is characterized by increases in the production of pro-inflammatory adipokines in the adipose tissue.To elucidate the effects of natural dietary components on the inflammatory and metabolic consequences of obesity, we examined the effects of unripe, ripe and industrial acerola juice (Malpighia emarginata DC.) on the relevant inflammatory and lipolysis proteins in the adipose tissue of mice with cafeteria diet-induced obesity.

Materials/methods: Two groups of male Swiss mice were fed on a standard diet (STA) or a cafeteria diet (CAF) for 13 weeks. Afterwards, the CAF-fed animals were divided into five subgroups, each of which received a different supplement for one further month (water, unripe acerola juice, ripe acerola juice, industrial acerola juice, or vitamin C) by gavage. Enzyme-linked immunosorbent assays, Western blotting, a colorimetric method and histology were utilized to assess the observed data.

Results: The CAF water (control obese) group showed a significant increase in their adiposity indices and triacylglycerol levels, in addition to a reduced IL-10/TNF-α ratio in the adipose tissue, compared with the control lean group. In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue. In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.

Conclusions: Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

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Related in: MedlinePlus

Effects of cafeteria diet on inflammatory molecules in epididymal adipose tissue. The levels of phosphorylated pIκB (a); pJNK (b); NF-κB (c); and TLR4 (d) in the adipose tissue of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerola (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C; @p < 0.05 versus the cafeteria diet with industrial acerola juice.
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Figure 3: Effects of cafeteria diet on inflammatory molecules in epididymal adipose tissue. The levels of phosphorylated pIκB (a); pJNK (b); NF-κB (c); and TLR4 (d) in the adipose tissue of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerola (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C; @p < 0.05 versus the cafeteria diet with industrial acerola juice.

Mentions: Next, we evaluated the protein levels of the molecules involved in the transduction of pro-inflammatory signals (pIκ-Bα, pJNK, NF-κB, TLR4), which are presented as representative bands. The pJNK protein levels were reduced in the CAF + IND, CAF + UNR, and CAF + RIP groups (p < 0.05; Figure 3b) when compared with the CAF + WD group. There was no difference among the groups in the NF-κBp50 protein levels (p > 0.05; Figure 3c). Hoping to evaluate whether lipolytic enzymes were altered by the treatment, we evaluated the levels of pAMPK, pHSLser563, pHSLser660, PeriA, CGI-58, and ATGL. The results demonstrated a higher phosphorylation of HSL at the serine 563 and 660 sites in the CAF + Vit C and CAF + IND groups (p < 0.05) when compared with the CAF + WD group, suggesting increased lipolytic activity in these groups (Figure 4).


Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet.

Dias FM, Leffa DD, Daumann F, Marques Sde O, Luciano TF, Possato JC, de Santana AA, Neves RX, Rosa JC, Oyama LM, Rodrigues B, de Andrade VM, de Souza CT, de Lira FS - Lipids Health Dis (2014)

Effects of cafeteria diet on inflammatory molecules in epididymal adipose tissue. The levels of phosphorylated pIκB (a); pJNK (b); NF-κB (c); and TLR4 (d) in the adipose tissue of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerola (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C; @p < 0.05 versus the cafeteria diet with industrial acerola juice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC3926336&req=5

Figure 3: Effects of cafeteria diet on inflammatory molecules in epididymal adipose tissue. The levels of phosphorylated pIκB (a); pJNK (b); NF-κB (c); and TLR4 (d) in the adipose tissue of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerola (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C; @p < 0.05 versus the cafeteria diet with industrial acerola juice.
Mentions: Next, we evaluated the protein levels of the molecules involved in the transduction of pro-inflammatory signals (pIκ-Bα, pJNK, NF-κB, TLR4), which are presented as representative bands. The pJNK protein levels were reduced in the CAF + IND, CAF + UNR, and CAF + RIP groups (p < 0.05; Figure 3b) when compared with the CAF + WD group. There was no difference among the groups in the NF-κBp50 protein levels (p > 0.05; Figure 3c). Hoping to evaluate whether lipolytic enzymes were altered by the treatment, we evaluated the levels of pAMPK, pHSLser563, pHSLser660, PeriA, CGI-58, and ATGL. The results demonstrated a higher phosphorylation of HSL at the serine 563 and 660 sites in the CAF + Vit C and CAF + IND groups (p < 0.05) when compared with the CAF + WD group, suggesting increased lipolytic activity in these groups (Figure 4).

Bottom Line: In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue.In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, 19060-900 Presidente Prudente, SP, Brazil. fabiolira@fct.unesp.br.

ABSTRACT

Background: Obesity has been studied as a metabolic and an inflammatory disease and is characterized by increases in the production of pro-inflammatory adipokines in the adipose tissue.To elucidate the effects of natural dietary components on the inflammatory and metabolic consequences of obesity, we examined the effects of unripe, ripe and industrial acerola juice (Malpighia emarginata DC.) on the relevant inflammatory and lipolysis proteins in the adipose tissue of mice with cafeteria diet-induced obesity.

Materials/methods: Two groups of male Swiss mice were fed on a standard diet (STA) or a cafeteria diet (CAF) for 13 weeks. Afterwards, the CAF-fed animals were divided into five subgroups, each of which received a different supplement for one further month (water, unripe acerola juice, ripe acerola juice, industrial acerola juice, or vitamin C) by gavage. Enzyme-linked immunosorbent assays, Western blotting, a colorimetric method and histology were utilized to assess the observed data.

Results: The CAF water (control obese) group showed a significant increase in their adiposity indices and triacylglycerol levels, in addition to a reduced IL-10/TNF-α ratio in the adipose tissue, compared with the control lean group. In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue. In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.

Conclusions: Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

Show MeSH
Related in: MedlinePlus