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Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet.

Dias FM, Leffa DD, Daumann F, Marques Sde O, Luciano TF, Possato JC, de Santana AA, Neves RX, Rosa JC, Oyama LM, Rodrigues B, de Andrade VM, de Souza CT, de Lira FS - Lipids Health Dis (2014)

Bottom Line: In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue.In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, 19060-900 Presidente Prudente, SP, Brazil. fabiolira@fct.unesp.br.

ABSTRACT

Background: Obesity has been studied as a metabolic and an inflammatory disease and is characterized by increases in the production of pro-inflammatory adipokines in the adipose tissue.To elucidate the effects of natural dietary components on the inflammatory and metabolic consequences of obesity, we examined the effects of unripe, ripe and industrial acerola juice (Malpighia emarginata DC.) on the relevant inflammatory and lipolysis proteins in the adipose tissue of mice with cafeteria diet-induced obesity.

Materials/methods: Two groups of male Swiss mice were fed on a standard diet (STA) or a cafeteria diet (CAF) for 13 weeks. Afterwards, the CAF-fed animals were divided into five subgroups, each of which received a different supplement for one further month (water, unripe acerola juice, ripe acerola juice, industrial acerola juice, or vitamin C) by gavage. Enzyme-linked immunosorbent assays, Western blotting, a colorimetric method and histology were utilized to assess the observed data.

Results: The CAF water (control obese) group showed a significant increase in their adiposity indices and triacylglycerol levels, in addition to a reduced IL-10/TNF-α ratio in the adipose tissue, compared with the control lean group. In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue. In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.

Conclusions: Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

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Related in: MedlinePlus

Effects of cafeteria diet on metabolic parameters. Analysis of body weight (g) (a); adiposity index (% of body weight) (b); and circulating TAG (mg/dL) (c) of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerolas (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C.
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Figure 1: Effects of cafeteria diet on metabolic parameters. Analysis of body weight (g) (a); adiposity index (% of body weight) (b); and circulating TAG (mg/dL) (c) of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerolas (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C.

Mentions: First, we evaluated the effects of the cafeteria diet on the body weight of the mice. The cafeteria diet increased the body weight in the CAF + WD group when compared with standard diet group (p < 0.01). Although the treatment not was efficient in reducing the weight of the experimental mice (p > 0.05; Figure 1a), an interesting result was observed in the adiposity index. The cafeteria diet markedly increased the adiposity index in the CAF + WD group when compared with STA group (p < 0.05), while the CAF + Vit C, CAF + IND, CAF + UNR, and CAF + RIP groups exhibited a reduced adiposity index when compared with the CAF + WD group (p < 0.05; Figure 1b). No significant difference was found among the treated groups (p > 0.05). To determine the adiposity index, the epididymal, mesenteric and retroperitoneal pads were weighed. To observe whether the treatment induced increased lipolysis, we assayed the triacylglycerol levels. The TAG levels were increased in the CAF + WD and CAF + Vit C groups when compared with the STA group (p < 0.05). However, the TAG levels in the CAF + IND, CAF + UNR, and CAF + RIP groups were reduced when compared with the CAF + WD and CAF + Vit C groups (p < 0.05; Figure 1c).


Acerola (Malpighia emarginata DC.) juice intake protects against alterations to proteins involved in inflammatory and lipolysis pathways in the adipose tissue of obese mice fed a cafeteria diet.

Dias FM, Leffa DD, Daumann F, Marques Sde O, Luciano TF, Possato JC, de Santana AA, Neves RX, Rosa JC, Oyama LM, Rodrigues B, de Andrade VM, de Souza CT, de Lira FS - Lipids Health Dis (2014)

Effects of cafeteria diet on metabolic parameters. Analysis of body weight (g) (a); adiposity index (% of body weight) (b); and circulating TAG (mg/dL) (c) of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerolas (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3926336&req=5

Figure 1: Effects of cafeteria diet on metabolic parameters. Analysis of body weight (g) (a); adiposity index (% of body weight) (b); and circulating TAG (mg/dL) (c) of mice fed the standard diet (STA), the cafeteria diet with distilled water (CAF + WD), the cafeteria diet with vitamin C (CAF + Vit C), the cafeteria diet with industrial acerola juice (CAF + IND), the cafeteria diet with the juice of unripe acerolas (CAF + UNR) or the cafeteria diet with the juice of ripe acerolas (CAF + RIP). The results are expressed as means ± SEM (n = 6 per group). $p < 0.05 versus standard diet; *p < 0.05 versus the cafeteria diet with distilled water; #p < 0.05 versus the cafeteria diet with vitamin C.
Mentions: First, we evaluated the effects of the cafeteria diet on the body weight of the mice. The cafeteria diet increased the body weight in the CAF + WD group when compared with standard diet group (p < 0.01). Although the treatment not was efficient in reducing the weight of the experimental mice (p > 0.05; Figure 1a), an interesting result was observed in the adiposity index. The cafeteria diet markedly increased the adiposity index in the CAF + WD group when compared with STA group (p < 0.05), while the CAF + Vit C, CAF + IND, CAF + UNR, and CAF + RIP groups exhibited a reduced adiposity index when compared with the CAF + WD group (p < 0.05; Figure 1b). No significant difference was found among the treated groups (p > 0.05). To determine the adiposity index, the epididymal, mesenteric and retroperitoneal pads were weighed. To observe whether the treatment induced increased lipolysis, we assayed the triacylglycerol levels. The TAG levels were increased in the CAF + WD and CAF + Vit C groups when compared with the STA group (p < 0.05). However, the TAG levels in the CAF + IND, CAF + UNR, and CAF + RIP groups were reduced when compared with the CAF + WD and CAF + Vit C groups (p < 0.05; Figure 1c).

Bottom Line: In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue.In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Rua Roberto Simonsen, 305, 19060-900 Presidente Prudente, SP, Brazil. fabiolira@fct.unesp.br.

ABSTRACT

Background: Obesity has been studied as a metabolic and an inflammatory disease and is characterized by increases in the production of pro-inflammatory adipokines in the adipose tissue.To elucidate the effects of natural dietary components on the inflammatory and metabolic consequences of obesity, we examined the effects of unripe, ripe and industrial acerola juice (Malpighia emarginata DC.) on the relevant inflammatory and lipolysis proteins in the adipose tissue of mice with cafeteria diet-induced obesity.

Materials/methods: Two groups of male Swiss mice were fed on a standard diet (STA) or a cafeteria diet (CAF) for 13 weeks. Afterwards, the CAF-fed animals were divided into five subgroups, each of which received a different supplement for one further month (water, unripe acerola juice, ripe acerola juice, industrial acerola juice, or vitamin C) by gavage. Enzyme-linked immunosorbent assays, Western blotting, a colorimetric method and histology were utilized to assess the observed data.

Results: The CAF water (control obese) group showed a significant increase in their adiposity indices and triacylglycerol levels, in addition to a reduced IL-10/TNF-α ratio in the adipose tissue, compared with the control lean group. In contrast, acerola juice and Vitamin C intake ameliorated the weight gain, reducing the TAG levels and increasing the IL-10/TNF-α ratio in adipose tissue. In addition, acerola juice intake led to reductions both in the level of phosphorylated JNK and to increases in the phosphorylation of IκBα and HSLser660 in adipose tissue.

Conclusions: Taken together, these results suggest that acerola juice reduces low-grade inflammation and ameliorates obesity-associated defects in the lipolytic processes.

Show MeSH
Related in: MedlinePlus