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Expression and prognostic significance of golgiglycoprotein73 (GP73) with epithelial-mesenchymal transition (EMT) related molecules in hepatocellular carcinoma (HCC).

Bao YX, Cao Q, Yang Y, Mao R, Xiao L, Zhang H, Zhao HR, Wen H - Diagn Pathol (2013)

Bottom Line: GP73 expression in HCC was higher compared with PCL tissues (χ2 = 73.60, P < 0.05).GP73 was negatively with E-cadherin and positively correlated with Vimentin.It might be associated with aggressive behavior of HCC and had influence on patients' OS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Tumor Department, First Affiliated Hospital of Xinjiang Medical University (XJMU), Urumqi, China. wenhao@126.com.

ABSTRACT

Background: Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third cause of cancer-related deaths, worldwide. It is essential to develop an effective prognostic biomarker and determine the mechanisms underlying HCC invasion and metastasis.

Aims: This study aimed to investigate the expression of Golgi glycoprotein73 (GP73) and Epithelial-mesenchymal transition (EMT) molecules such as E-cadherin and Vimentin in HCC. We also evaluated the prognostic value of GP73 in HCC.

Methods: Immunohistochemistry (IHC) was used to determine the expression of GP73 and EMT molecules in 75 HCC specimens and the corresponding paracarcinomatous liver (PCL) tissues. Spearman's correlation test was used to analyze the correlation of GP73 and EMT molecules. Clinicopathological features of the HCC patients were also analyzed. Univariate survival analysis was performed by the Kaplan-Meier method and differences among the groups were analyzed by the Log-rank test.

Results: GP73 expression in HCC was higher compared with PCL tissues (χ2 = 73.60, P < 0.05). EMT molecules were also detected in HCC and PCL tissues. GP73 was negatively correlated with E-cadherin (r = - 0.49, P < 0.05), but positively correlated with Vimentin (r = 0.46, P < 0.05) in HCC. GP73 was correlated with the clinicopathological features including Edmondson grade, vascular invasion and TNM stage (P < 0.05), which was also associated with overall survival (OS) (P < 0.05).

Conclusions: GP73 was negatively with E-cadherin and positively correlated with Vimentin. It might be associated with aggressive behavior of HCC and had influence on patients' OS. Further research is needed to determine the potential of GP73.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/29 vs/1504046946108618; http://med.motic.com/MoticGallery/Slide?id=3b6a037e-f60e-4c68-9106-41e790de9431&user=2C69F0D6-A478-4A2B-ABF0-BB36763E8025; http://med.motic.com/MoticGallery/Slide?id=a25b5b32-b613-47b0-9f8b-e1e67a95d1bf&user=2C69F0D6-A478-4A2B-ABF0-BB36763E8025.

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Immunohistochemistry of E-cadherin, Vimentin and GP73 in PCL tissues and HCC tissue (400×). E-cadherin was detected in the cell membrane. Note that E-cadherin showed moderate and strong staining in s (PCL) (1) By contrast, it was almost absent in normal control HCC(2) (×400).Vimentin was detected in the cytoplasm and interstitial cells. Note that Vimentin was almost absent in normal controls (PCL) (3) By contrast, moderate and strong staining was seen in HCC (4) (×400).GP73 protein expression was evaluated by an En-Vision immunohistochemical method. Note that GP73 was almost absent in normal controls (PCL) (5). By contrast, moderate and strong staining of GP73 was identified in HCC, respectively. (×400). (6).
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Figure 1: Immunohistochemistry of E-cadherin, Vimentin and GP73 in PCL tissues and HCC tissue (400×). E-cadherin was detected in the cell membrane. Note that E-cadherin showed moderate and strong staining in s (PCL) (1) By contrast, it was almost absent in normal control HCC(2) (×400).Vimentin was detected in the cytoplasm and interstitial cells. Note that Vimentin was almost absent in normal controls (PCL) (3) By contrast, moderate and strong staining was seen in HCC (4) (×400).GP73 protein expression was evaluated by an En-Vision immunohistochemical method. Note that GP73 was almost absent in normal controls (PCL) (5). By contrast, moderate and strong staining of GP73 was identified in HCC, respectively. (×400). (6).

Mentions: GP73 was detected in the cytoplasm of liver cells, which is brown. E-cadherin was detected as a membrane-linear/disarrayed immunostaining pattern, while Vimentin labeling was observed in cytoplasmic and interstitial cells (Figure 1). Data showed that GP73 staining was detected up to 72% (54/75) in HCC, but only up to 4% (3/75) in the PCL tissues. GP73 expressed in HCC was higher compared with PCL (χ2 = 73.60, P < 0.05). The expression of E-cadherin in HCC was 24% (18/75) compared with PCL tissues 88% (66/75). The increased expression of Vimentin in HCC [60% (45/75)] was higher than in PCL tissues [12% (9/75)] (Table 1).


Expression and prognostic significance of golgiglycoprotein73 (GP73) with epithelial-mesenchymal transition (EMT) related molecules in hepatocellular carcinoma (HCC).

Bao YX, Cao Q, Yang Y, Mao R, Xiao L, Zhang H, Zhao HR, Wen H - Diagn Pathol (2013)

Immunohistochemistry of E-cadherin, Vimentin and GP73 in PCL tissues and HCC tissue (400×). E-cadherin was detected in the cell membrane. Note that E-cadherin showed moderate and strong staining in s (PCL) (1) By contrast, it was almost absent in normal control HCC(2) (×400).Vimentin was detected in the cytoplasm and interstitial cells. Note that Vimentin was almost absent in normal controls (PCL) (3) By contrast, moderate and strong staining was seen in HCC (4) (×400).GP73 protein expression was evaluated by an En-Vision immunohistochemical method. Note that GP73 was almost absent in normal controls (PCL) (5). By contrast, moderate and strong staining of GP73 was identified in HCC, respectively. (×400). (6).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3924912&req=5

Figure 1: Immunohistochemistry of E-cadherin, Vimentin and GP73 in PCL tissues and HCC tissue (400×). E-cadherin was detected in the cell membrane. Note that E-cadherin showed moderate and strong staining in s (PCL) (1) By contrast, it was almost absent in normal control HCC(2) (×400).Vimentin was detected in the cytoplasm and interstitial cells. Note that Vimentin was almost absent in normal controls (PCL) (3) By contrast, moderate and strong staining was seen in HCC (4) (×400).GP73 protein expression was evaluated by an En-Vision immunohistochemical method. Note that GP73 was almost absent in normal controls (PCL) (5). By contrast, moderate and strong staining of GP73 was identified in HCC, respectively. (×400). (6).
Mentions: GP73 was detected in the cytoplasm of liver cells, which is brown. E-cadherin was detected as a membrane-linear/disarrayed immunostaining pattern, while Vimentin labeling was observed in cytoplasmic and interstitial cells (Figure 1). Data showed that GP73 staining was detected up to 72% (54/75) in HCC, but only up to 4% (3/75) in the PCL tissues. GP73 expressed in HCC was higher compared with PCL (χ2 = 73.60, P < 0.05). The expression of E-cadherin in HCC was 24% (18/75) compared with PCL tissues 88% (66/75). The increased expression of Vimentin in HCC [60% (45/75)] was higher than in PCL tissues [12% (9/75)] (Table 1).

Bottom Line: GP73 expression in HCC was higher compared with PCL tissues (χ2 = 73.60, P < 0.05).GP73 was negatively with E-cadherin and positively correlated with Vimentin.It might be associated with aggressive behavior of HCC and had influence on patients' OS.

View Article: PubMed Central - HTML - PubMed

Affiliation: Tumor Department, First Affiliated Hospital of Xinjiang Medical University (XJMU), Urumqi, China. wenhao@126.com.

ABSTRACT

Background: Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third cause of cancer-related deaths, worldwide. It is essential to develop an effective prognostic biomarker and determine the mechanisms underlying HCC invasion and metastasis.

Aims: This study aimed to investigate the expression of Golgi glycoprotein73 (GP73) and Epithelial-mesenchymal transition (EMT) molecules such as E-cadherin and Vimentin in HCC. We also evaluated the prognostic value of GP73 in HCC.

Methods: Immunohistochemistry (IHC) was used to determine the expression of GP73 and EMT molecules in 75 HCC specimens and the corresponding paracarcinomatous liver (PCL) tissues. Spearman's correlation test was used to analyze the correlation of GP73 and EMT molecules. Clinicopathological features of the HCC patients were also analyzed. Univariate survival analysis was performed by the Kaplan-Meier method and differences among the groups were analyzed by the Log-rank test.

Results: GP73 expression in HCC was higher compared with PCL tissues (χ2 = 73.60, P < 0.05). EMT molecules were also detected in HCC and PCL tissues. GP73 was negatively correlated with E-cadherin (r = - 0.49, P < 0.05), but positively correlated with Vimentin (r = 0.46, P < 0.05) in HCC. GP73 was correlated with the clinicopathological features including Edmondson grade, vascular invasion and TNM stage (P < 0.05), which was also associated with overall survival (OS) (P < 0.05).

Conclusions: GP73 was negatively with E-cadherin and positively correlated with Vimentin. It might be associated with aggressive behavior of HCC and had influence on patients' OS. Further research is needed to determine the potential of GP73.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/29 vs/1504046946108618; http://med.motic.com/MoticGallery/Slide?id=3b6a037e-f60e-4c68-9106-41e790de9431&user=2C69F0D6-A478-4A2B-ABF0-BB36763E8025; http://med.motic.com/MoticGallery/Slide?id=a25b5b32-b613-47b0-9f8b-e1e67a95d1bf&user=2C69F0D6-A478-4A2B-ABF0-BB36763E8025.

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Related in: MedlinePlus