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Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus

Imaging at 764 [nm] of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.
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Figure 8: Imaging at 764 [nm] of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.

Mentions: In order to obtain quantitative information regarding the pigment production curves of Figure7, some considerations need to be taken into account. The absorbance at 580 [nm] has simultaneous information of pigment and bacterial cell components. Thus, a band centered at 764 [nm] which corresponds to the minimal pigment absorption, is now selected. The absorbance intensity signal versus time for both strains at 764 [nm] can be seen in Figure8.


Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Imaging at 764 [nm] of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924903&req=5

Figure 8: Imaging at 764 [nm] of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.
Mentions: In order to obtain quantitative information regarding the pigment production curves of Figure7, some considerations need to be taken into account. The absorbance at 580 [nm] has simultaneous information of pigment and bacterial cell components. Thus, a band centered at 764 [nm] which corresponds to the minimal pigment absorption, is now selected. The absorbance intensity signal versus time for both strains at 764 [nm] can be seen in Figure8.

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus