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Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus

Imaging at 580 nm of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.
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Figure 7: Imaging at 580 nm of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.

Mentions: With the aim to resolve the spectral differences between the strains associated with the pigment production, we use a PBHCs that allows to obtain spatio-spectral information without disturbing the sample. The experiment is developed by plating both strains in LB-agar at equal initial cell concentration, and following the methodology explained in the previous section. In particular, two spectral bands are selected and the subsequent temporal samples have information regarding the biological process. To obtain the signal, a band centered at 580 [nm] which corresponds to the wavelength of maximal absorption, is selected. In Figure7c is shown the absorbance intensity signal versus time for both strains. Now the pigment absorbance signal is successfully retrieved and is clearly differentiable between the strains. The absorbance pigment intensity signal of the C. violaceum wild-type strain exhibits a bacterial growing-like curve behavior.


Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Imaging at 580 nm of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924903&req=5

Figure 7: Imaging at 580 nm of both C. violaceum wild-type strain (a) and C. violaceum CV026 strain (b). Hyperspectral measurements in (c) show the absorbance intensity signal versus time for both strains.
Mentions: With the aim to resolve the spectral differences between the strains associated with the pigment production, we use a PBHCs that allows to obtain spatio-spectral information without disturbing the sample. The experiment is developed by plating both strains in LB-agar at equal initial cell concentration, and following the methodology explained in the previous section. In particular, two spectral bands are selected and the subsequent temporal samples have information regarding the biological process. To obtain the signal, a band centered at 580 [nm] which corresponds to the wavelength of maximal absorption, is selected. In Figure7c is shown the absorbance intensity signal versus time for both strains. Now the pigment absorbance signal is successfully retrieved and is clearly differentiable between the strains. The absorbance pigment intensity signal of the C. violaceum wild-type strain exhibits a bacterial growing-like curve behavior.

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus