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Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus

Spectrophotometric measurement of both C. violaceum wild-type strain and C. violaceum CV026 strain in liquid culture. Data shows the absorbance intensity signal versus wavelength. Data is taken during the stationary phase of growing, seven hours after inoculation.
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Figure 6: Spectrophotometric measurement of both C. violaceum wild-type strain and C. violaceum CV026 strain in liquid culture. Data shows the absorbance intensity signal versus wavelength. Data is taken during the stationary phase of growing, seven hours after inoculation.

Mentions: Although both samples are differentiable trough simple eye-inspection, the absorbance intensity signal shown in Figure6 is poor for quantification. This constraint is due to the screening by the turbid media itself which is a result of optical absorption in the visible spectrum by the bacterial cell components (membranes, organelles, DNA, proteins, etc.) (Gerhardt1994; Koch1970).


Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Spectrophotometric measurement of both C. violaceum wild-type strain and C. violaceum CV026 strain in liquid culture. Data shows the absorbance intensity signal versus wavelength. Data is taken during the stationary phase of growing, seven hours after inoculation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924903&req=5

Figure 6: Spectrophotometric measurement of both C. violaceum wild-type strain and C. violaceum CV026 strain in liquid culture. Data shows the absorbance intensity signal versus wavelength. Data is taken during the stationary phase of growing, seven hours after inoculation.
Mentions: Although both samples are differentiable trough simple eye-inspection, the absorbance intensity signal shown in Figure6 is poor for quantification. This constraint is due to the screening by the turbid media itself which is a result of optical absorption in the visible spectrum by the bacterial cell components (membranes, organelles, DNA, proteins, etc.) (Gerhardt1994; Koch1970).

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus