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Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus

Selected region of interest in the reasembled spectral image at 580 [nm] at a) t = 0 hrs and b) t = 37 hrs.
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Figure 3: Selected region of interest in the reasembled spectral image at 580 [nm] at a) t = 0 hrs and b) t = 37 hrs.

Mentions: where Yr(i,λj,k) is the white reference value and is the estimated baseline obtained when measuring the dark reference. Recalling Eq. 1, by subtracting in Eq. 2, the baseline value is compensated. Further, the normalization between the measured sample and the white reference compensates for the system spectral response and the term a(i,j). With the spectral reflectance at hand, the absorbance values A(i,λj,k) are retrieved and the maximal and minimal pigment absorption bands are selected, that in our case are 580 [nm] and 764 [nm], respectively. Therefore, to analyze the spectral behavior of the samples taken at different times, first it is required to reassemble the spatial information from the hypercube. Then, a region of interest region of interest (RI) is located on each sample, covering a spatial region equal to 21 by 21 pixels, as shown in Figure3.


Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Selected region of interest in the reasembled spectral image at 580 [nm] at a) t = 0 hrs and b) t = 37 hrs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924903&req=5

Figure 3: Selected region of interest in the reasembled spectral image at 580 [nm] at a) t = 0 hrs and b) t = 37 hrs.
Mentions: where Yr(i,λj,k) is the white reference value and is the estimated baseline obtained when measuring the dark reference. Recalling Eq. 1, by subtracting in Eq. 2, the baseline value is compensated. Further, the normalization between the measured sample and the white reference compensates for the system spectral response and the term a(i,j). With the spectral reflectance at hand, the absorbance values A(i,λj,k) are retrieved and the maximal and minimal pigment absorption bands are selected, that in our case are 580 [nm] and 764 [nm], respectively. Therefore, to analyze the spectral behavior of the samples taken at different times, first it is required to reassemble the spatial information from the hypercube. Then, a region of interest region of interest (RI) is located on each sample, covering a spatial region equal to 21 by 21 pixels, as shown in Figure3.

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus