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Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus

Laboratory prototype to retrieve the spatio-spectral image. The aluminum arc holds the illumination and camera system. The target scene lies on a movable base where the arrow indicates scan direction. Imaging is acquired during the scan process and data analysis is post-processed.
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Figure 2: Laboratory prototype to retrieve the spatio-spectral image. The aluminum arc holds the illumination and camera system. The target scene lies on a movable base where the arrow indicates scan direction. Imaging is acquired during the scan process and data analysis is post-processed.

Mentions: In order to acquire image sequences with high spectral resolution, The set-up illustrated in Figure2 is constructed, where the key component is the PBHC. Such a camera can be described, in brief, as an optoelectronic imaging system composed of four sub-systems: the optics, the spectrograph, the sensor, and the readout electronics (Borengasser et al.2008). Unlike broadband images, in PBHC the spatial information is mapped onto one axis of the focal plane array (FPA), while the spectral information is mapped onto the second axis. Therefore, a biological sample (now target scene) must be scanned one line at a time by displacing the camera in an orthogonal trajectory with respect to the axis used to encode the spectral information.


Characterization of Chromobacterium violaceum pigment through a hyperspectral imaging system.

Gallardo MJ, Staforelli JP, Meza P, Bordeu I, Torres S - AMB Express (2014)

Laboratory prototype to retrieve the spatio-spectral image. The aluminum arc holds the illumination and camera system. The target scene lies on a movable base where the arrow indicates scan direction. Imaging is acquired during the scan process and data analysis is post-processed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924903&req=5

Figure 2: Laboratory prototype to retrieve the spatio-spectral image. The aluminum arc holds the illumination and camera system. The target scene lies on a movable base where the arrow indicates scan direction. Imaging is acquired during the scan process and data analysis is post-processed.
Mentions: In order to acquire image sequences with high spectral resolution, The set-up illustrated in Figure2 is constructed, where the key component is the PBHC. Such a camera can be described, in brief, as an optoelectronic imaging system composed of four sub-systems: the optics, the spectrograph, the sensor, and the readout electronics (Borengasser et al.2008). Unlike broadband images, in PBHC the spatial information is mapped onto one axis of the focal plane array (FPA), while the spectral information is mapped onto the second axis. Therefore, a biological sample (now target scene) must be scanned one line at a time by displacing the camera in an orthogonal trajectory with respect to the axis used to encode the spectral information.

Bottom Line: This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony.Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized.Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Optics and Photonics, Universidad de Concepción, Concepción, Chile. mgallardo@cefop.udec.cl.

ABSTRACT
In this paper, a comprehensive spatio-spectral and temporal analysis for Chromobacterium violaceum colonies is reported. A hyperspectral imaging (HSI) system is used to recover the spectral signatures of pigment production in a non-homogeneous media with high spectral resolution and high sensitivity in vivo, without destructing the sample. This non-contact sensing technique opens avenues to study the temporal growing of a specific section in the bacterial colony. Further, from a 580 [nm] and 764 [nm] spatio-spectral time series, a wild-type and mutant Chromobacterium violaceum strains are characterized. Such study provides quantitative information about kinetic parameters of pigment production and bacterial growing.

No MeSH data available.


Related in: MedlinePlus