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Is bone transplantation the gold standard for repair of alveolar bone defects?

Raposo-Amaral CE, Bueno DF, Almeida AB, Jorgetti V, Costa CC, Gouveia CH, Vulcano LC, Fanganiello RD, Passos-Bueno MR, Alonso N - J Tissue Eng (2014)

Bottom Line: Groups 2-5 were compared to Group 1, the reference group.Groups 4 and 5 showed 51.48% ± 11.7% (p = 0.30) and 61.80% ± 2.14% (p = 0.88) of bone in the defect, respectively.Animals whose bone defects were repaired with α-tricalcium phosphate and mesenchymal stem cells presented the highest bone volume filling the defects; both were not statistically different from autogenous bone.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirurgia Plástica e Queimaduras, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, Brazil.

ABSTRACT
New strategies to fulfill craniofacial bone defects have gained attention in recent years due to the morbidity of autologous bone graft harvesting. We aimed to evaluate the in vivo efficacy of bone tissue engineering strategy using mesenchymal stem cells associated with two matrices (bovine bone mineral and α-tricalcium phosphate), compared to an autologous bone transfer. A total of 28 adult, male, non-immunosuppressed Wistar rats underwent a critical-sized osseous defect of 5 mm diameter in the alveolar region. Animals were divided into five groups. Group 1 (n = 7) defects were repaired with autogenous bone grafts; Group 2 (n = 5) defects were repaired with bovine bone mineral free of cells; Group 3 (n = 5) defects were repaired with bovine bone mineral loaded with mesenchymal stem cells; Group 4 (n = 5) defects were repaired with α-tricalcium phosphate free of cells; and Group 5 (n = 6) defects were repaired with α-tricalcium phosphate loaded with mesenchymal stem cells. Groups 2-5 were compared to Group 1, the reference group. Healing response was evaluated by histomorphometry and computerized tomography. Histomorphometrically, Group 1 showed 60.27% ± 16.13% of bone in the defect. Groups 2 and 3 showed 23.02% ± 8.6% (p = 0.01) and 38.35% ± 19.59% (p = 0.06) of bone in the defect, respectively. Groups 4 and 5 showed 51.48% ± 11.7% (p = 0.30) and 61.80% ± 2.14% (p = 0.88) of bone in the defect, respectively. Animals whose bone defects were repaired with α-tricalcium phosphate and mesenchymal stem cells presented the highest bone volume filling the defects; both were not statistically different from autogenous bone.

No MeSH data available.


Toluidine blue staining of representative sagittal sections of alveolar osseous defects at 8 weeks after surgery in each group. Woven bone is displayed in all samples (from left to right; Groups 1, 2, 3, 4, and 5, respectively, under 400× magnification).TCP: tricalcium phosphate; MSC: mesenchymal stem cell.
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fig5-2041731413519352: Toluidine blue staining of representative sagittal sections of alveolar osseous defects at 8 weeks after surgery in each group. Woven bone is displayed in all samples (from left to right; Groups 1, 2, 3, 4, and 5, respectively, under 400× magnification).TCP: tricalcium phosphate; MSC: mesenchymal stem cell.

Mentions: Through histomorphometric analysis, at 8 weeks post-surgery, we observed average bone volumes in the alveolar defect of 60.27% ± 16.13% for Group 1, 23.02% ± 8.6% for Group 2, 38.35% ± 19.59% for Group 3, 51.48% ± 11.7% for Group 4, and 61.80% ± 2.14% for Group 5. The bone volume of Group 1 was not statistically different from Group 4 (p = 0.3) or Group 5 (p = 0.88), whereas it statistically differed from Group 2 (p = 0.01) and presented a trend toward statistical difference from Group 3 (p = 0.06). The animals of Group 3 presented a trend to generate statistically more bone than Group 2 (p = 0.06), whereas Group 5 did not present statistically more bone than Group 4 (p > 0.05) (Figures 5 and 6).


Is bone transplantation the gold standard for repair of alveolar bone defects?

Raposo-Amaral CE, Bueno DF, Almeida AB, Jorgetti V, Costa CC, Gouveia CH, Vulcano LC, Fanganiello RD, Passos-Bueno MR, Alonso N - J Tissue Eng (2014)

Toluidine blue staining of representative sagittal sections of alveolar osseous defects at 8 weeks after surgery in each group. Woven bone is displayed in all samples (from left to right; Groups 1, 2, 3, 4, and 5, respectively, under 400× magnification).TCP: tricalcium phosphate; MSC: mesenchymal stem cell.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3924878&req=5

fig5-2041731413519352: Toluidine blue staining of representative sagittal sections of alveolar osseous defects at 8 weeks after surgery in each group. Woven bone is displayed in all samples (from left to right; Groups 1, 2, 3, 4, and 5, respectively, under 400× magnification).TCP: tricalcium phosphate; MSC: mesenchymal stem cell.
Mentions: Through histomorphometric analysis, at 8 weeks post-surgery, we observed average bone volumes in the alveolar defect of 60.27% ± 16.13% for Group 1, 23.02% ± 8.6% for Group 2, 38.35% ± 19.59% for Group 3, 51.48% ± 11.7% for Group 4, and 61.80% ± 2.14% for Group 5. The bone volume of Group 1 was not statistically different from Group 4 (p = 0.3) or Group 5 (p = 0.88), whereas it statistically differed from Group 2 (p = 0.01) and presented a trend toward statistical difference from Group 3 (p = 0.06). The animals of Group 3 presented a trend to generate statistically more bone than Group 2 (p = 0.06), whereas Group 5 did not present statistically more bone than Group 4 (p > 0.05) (Figures 5 and 6).

Bottom Line: Groups 2-5 were compared to Group 1, the reference group.Groups 4 and 5 showed 51.48% ± 11.7% (p = 0.30) and 61.80% ± 2.14% (p = 0.88) of bone in the defect, respectively.Animals whose bone defects were repaired with α-tricalcium phosphate and mesenchymal stem cells presented the highest bone volume filling the defects; both were not statistically different from autogenous bone.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Cirurgia Plástica e Queimaduras, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, Brazil.

ABSTRACT
New strategies to fulfill craniofacial bone defects have gained attention in recent years due to the morbidity of autologous bone graft harvesting. We aimed to evaluate the in vivo efficacy of bone tissue engineering strategy using mesenchymal stem cells associated with two matrices (bovine bone mineral and α-tricalcium phosphate), compared to an autologous bone transfer. A total of 28 adult, male, non-immunosuppressed Wistar rats underwent a critical-sized osseous defect of 5 mm diameter in the alveolar region. Animals were divided into five groups. Group 1 (n = 7) defects were repaired with autogenous bone grafts; Group 2 (n = 5) defects were repaired with bovine bone mineral free of cells; Group 3 (n = 5) defects were repaired with bovine bone mineral loaded with mesenchymal stem cells; Group 4 (n = 5) defects were repaired with α-tricalcium phosphate free of cells; and Group 5 (n = 6) defects were repaired with α-tricalcium phosphate loaded with mesenchymal stem cells. Groups 2-5 were compared to Group 1, the reference group. Healing response was evaluated by histomorphometry and computerized tomography. Histomorphometrically, Group 1 showed 60.27% ± 16.13% of bone in the defect. Groups 2 and 3 showed 23.02% ± 8.6% (p = 0.01) and 38.35% ± 19.59% (p = 0.06) of bone in the defect, respectively. Groups 4 and 5 showed 51.48% ± 11.7% (p = 0.30) and 61.80% ± 2.14% (p = 0.88) of bone in the defect, respectively. Animals whose bone defects were repaired with α-tricalcium phosphate and mesenchymal stem cells presented the highest bone volume filling the defects; both were not statistically different from autogenous bone.

No MeSH data available.