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The function of e-cadherin in stem cell pluripotency and self-renewal.

Soncin F, Ward CM - Genes (Basel) (2011)

Bottom Line: E-cadherin; pluripotency; embryonic stem cell; induced pluripotent stem cell; iPS; ES; signaling pathways; Activin; Nodal.

View Article: PubMed Central - PubMed

Affiliation: Core Technology Facility, Faculty of Medical and Human Sciences, The University of Manchester, 46 Grafton Street, M13 9NT, UK. fsoncin@sanfordburnham.org.

ABSTRACT
E-cadherin; pluripotency; embryonic stem cell; induced pluripotent stem cell; iPS; ES; signaling pathways; Activin; Nodal.

No MeSH data available.


Diagrammatic representation of Activin, Nodal, TGFβ and BMP signaling pathways.
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f3-genes-02-00229: Diagrammatic representation of Activin, Nodal, TGFβ and BMP signaling pathways.

Mentions: Albeit important, LIF alone is not sufficient to maintain ES cells in the absence of serum [64]. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor beta (TGFβ) family, works in tandem with LIF in serum-free conditions to maintain ES cell pluripotency. BMP4 signals through a branch of the TGFβ cascade which involves the activation of the transduction molecules Smad1/5/8, which have been shown to activate the inhibitor of differentiation (Id) proteins (Figure 3) [64]. Ying and colleagues [64] have shown that overexpression of Id proteins allow culture of mES cells in the presence of LIF and absence of either serum or BMP4. They have subsequently demonstrated that mES cells can be cultured in the absence of LIF and BMP4 in medium supplemented with antagonists of mitogen-activated protein kinase (ERK1/2) and glycogen synthase kinase 3 (GSK3), defining a “ground state” of ES cell self-renewal, which is independent of exogenous factor supplementation [63].


The function of e-cadherin in stem cell pluripotency and self-renewal.

Soncin F, Ward CM - Genes (Basel) (2011)

Diagrammatic representation of Activin, Nodal, TGFβ and BMP signaling pathways.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924836&req=5

f3-genes-02-00229: Diagrammatic representation of Activin, Nodal, TGFβ and BMP signaling pathways.
Mentions: Albeit important, LIF alone is not sufficient to maintain ES cells in the absence of serum [64]. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor beta (TGFβ) family, works in tandem with LIF in serum-free conditions to maintain ES cell pluripotency. BMP4 signals through a branch of the TGFβ cascade which involves the activation of the transduction molecules Smad1/5/8, which have been shown to activate the inhibitor of differentiation (Id) proteins (Figure 3) [64]. Ying and colleagues [64] have shown that overexpression of Id proteins allow culture of mES cells in the presence of LIF and absence of either serum or BMP4. They have subsequently demonstrated that mES cells can be cultured in the absence of LIF and BMP4 in medium supplemented with antagonists of mitogen-activated protein kinase (ERK1/2) and glycogen synthase kinase 3 (GSK3), defining a “ground state” of ES cell self-renewal, which is independent of exogenous factor supplementation [63].

Bottom Line: E-cadherin; pluripotency; embryonic stem cell; induced pluripotent stem cell; iPS; ES; signaling pathways; Activin; Nodal.

View Article: PubMed Central - PubMed

Affiliation: Core Technology Facility, Faculty of Medical and Human Sciences, The University of Manchester, 46 Grafton Street, M13 9NT, UK. fsoncin@sanfordburnham.org.

ABSTRACT
E-cadherin; pluripotency; embryonic stem cell; induced pluripotent stem cell; iPS; ES; signaling pathways; Activin; Nodal.

No MeSH data available.