Limits...
Meiosis in a bottle: new approaches to overcome Mammalian meiocyte study limitations.

Roig I, Brieno-Enriquez MA, Caldes MG - Genes (Basel) (2011)

Bottom Line: The study of meiosis is limited because of the intrinsic nature of gametogenesis in mammals.One way to overcome these limitations would be the use of culture systems that would allow meiotic progression in vitro.In this review we will summarize all the efforts to-date in order to culture mammalian sperm and oocyte precursor cells.

View Article: PubMed Central - PubMed

Affiliation: Cytology and Histology Unit, Cell Biology, Physiology and Immunology Department, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain. ignasi.roig@uab.cat.

ABSTRACT
The study of meiosis is limited because of the intrinsic nature of gametogenesis in mammals. One way to overcome these limitations would be the use of culture systems that would allow meiotic progression in vitro. There have been some attempts to culture mammalian meiocytes in recent years. In this review we will summarize all the efforts to-date in order to culture mammalian sperm and oocyte precursor cells.

No MeSH data available.


Related in: MedlinePlus

Follicle development in the adult mouse ovary. Primordial (A), primary (B), pre-antral (C) and antral (D) follicles stained with PAS-hematoxilin [42].
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f1-genes-02-00152: Follicle development in the adult mouse ovary. Primordial (A), primary (B), pre-antral (C) and antral (D) follicles stained with PAS-hematoxilin [42].

Mentions: Adult mammalian ovaries contain follicles, which are the structures in which a post-prophase I oocyte develops. They consist of the oocyte themselves, surrounded by granulose and theca cells. In the adult ovary, one can find follicles at different developmental stages, the most abundant are primordial follicles. Based in morphology, human follicles can be classified (Figure 1, reviewed by [41]). Human follicles can be classified as primordial follicles (those that contain a primary oocyte embraced by flattened granulosa cells, Figure 1A), primary follicles (have a full cuboidal granulosa cell layer, Figure 1B), preantral follicles (growing primary oocytes enclosed by several granulosa cell layers, Figure 1C) and antral follicles (which contain a cavity with follicular fluid, granulosa cells differentiate into mural and cumulus cells, Figure 1D). Except for the oocytes from antral follicles, that usually need a short period of manipulation and maturation in culture, oocytes from small antral follicles and pre-antral follicles require long periods of growth in vitro to acquire developmental competence. There are many publications related to this field, in this sense we will only mention some of the most representative ones.


Meiosis in a bottle: new approaches to overcome Mammalian meiocyte study limitations.

Roig I, Brieno-Enriquez MA, Caldes MG - Genes (Basel) (2011)

Follicle development in the adult mouse ovary. Primordial (A), primary (B), pre-antral (C) and antral (D) follicles stained with PAS-hematoxilin [42].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924833&req=5

f1-genes-02-00152: Follicle development in the adult mouse ovary. Primordial (A), primary (B), pre-antral (C) and antral (D) follicles stained with PAS-hematoxilin [42].
Mentions: Adult mammalian ovaries contain follicles, which are the structures in which a post-prophase I oocyte develops. They consist of the oocyte themselves, surrounded by granulose and theca cells. In the adult ovary, one can find follicles at different developmental stages, the most abundant are primordial follicles. Based in morphology, human follicles can be classified (Figure 1, reviewed by [41]). Human follicles can be classified as primordial follicles (those that contain a primary oocyte embraced by flattened granulosa cells, Figure 1A), primary follicles (have a full cuboidal granulosa cell layer, Figure 1B), preantral follicles (growing primary oocytes enclosed by several granulosa cell layers, Figure 1C) and antral follicles (which contain a cavity with follicular fluid, granulosa cells differentiate into mural and cumulus cells, Figure 1D). Except for the oocytes from antral follicles, that usually need a short period of manipulation and maturation in culture, oocytes from small antral follicles and pre-antral follicles require long periods of growth in vitro to acquire developmental competence. There are many publications related to this field, in this sense we will only mention some of the most representative ones.

Bottom Line: The study of meiosis is limited because of the intrinsic nature of gametogenesis in mammals.One way to overcome these limitations would be the use of culture systems that would allow meiotic progression in vitro.In this review we will summarize all the efforts to-date in order to culture mammalian sperm and oocyte precursor cells.

View Article: PubMed Central - PubMed

Affiliation: Cytology and Histology Unit, Cell Biology, Physiology and Immunology Department, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain. ignasi.roig@uab.cat.

ABSTRACT
The study of meiosis is limited because of the intrinsic nature of gametogenesis in mammals. One way to overcome these limitations would be the use of culture systems that would allow meiotic progression in vitro. There have been some attempts to culture mammalian meiocytes in recent years. In this review we will summarize all the efforts to-date in order to culture mammalian sperm and oocyte precursor cells.

No MeSH data available.


Related in: MedlinePlus