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Biocontrol of Fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists.

Shi C, Yan P, Li J, Wu H, Li Q, Guan S - Int J Environ Res Public Health (2014)

Bottom Line: Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens.The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels.As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

View Article: PubMed Central - PubMed

Affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China. shicjwh@126.com.

ABSTRACT
Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

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Related in: MedlinePlus

The mycelia growth of F. graminearum in wheat kernels with bacterial cell-free culture supernatant (strain WPS4-1) and deionized water (control) added. (a) four days of culture, (b) ten days of culture and (c) eighteen days of culture.
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ijerph-11-01094-f002: The mycelia growth of F. graminearum in wheat kernels with bacterial cell-free culture supernatant (strain WPS4-1) and deionized water (control) added. (a) four days of culture, (b) ten days of culture and (c) eighteen days of culture.

Mentions: Based on the results of inhibition of F. graminearum growth, twenty bacterial strains were tested. After four days of incubation, there were some mycelia growing in the control wheat kernels with deionized water instead of the bacterial cell-free culture supernatants, while no mycelia were observed on the wheat kernels with the tested bacterial cell-free culture supernatants in the flasks (Figure 2a). On the tenth day, there were obvious differences in the antagonistic activities of the tested bacterial strains. In the flasks with the culture supernatants of some strains, such as WPP1-1 and HPP8, the inhibition activity was weak; whereas to some strains such as WPP10 and WPS4-1, the mycelia were obviously less than those in the control (Figure 2b). After 18 days’ of incubation, the wheat kernels were all covered with mycelia both in the control and tested flasks, and there were no differences of the amount of mycelia visibly (Figure 2c). The amount of DON produced in wheat kernels was determined by HPLC, and five strains XPP6-1, WPP1-2, WPP9, WPP10 and WPS4-1 with strong antagonistic activity against DON production were confirmed, with strain WPS4-1 showing the highest inhibition rate of 90.30% ± 1.01%, followed by strain WPP9 with an inhibition rate of 88.40% ± 4.41%. Strains WPP1-1 and HPP8 showed little inhibition activity towards the DON production in wheat kernels (Table 1).


Biocontrol of Fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists.

Shi C, Yan P, Li J, Wu H, Li Q, Guan S - Int J Environ Res Public Health (2014)

The mycelia growth of F. graminearum in wheat kernels with bacterial cell-free culture supernatant (strain WPS4-1) and deionized water (control) added. (a) four days of culture, (b) ten days of culture and (c) eighteen days of culture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924494&req=5

ijerph-11-01094-f002: The mycelia growth of F. graminearum in wheat kernels with bacterial cell-free culture supernatant (strain WPS4-1) and deionized water (control) added. (a) four days of culture, (b) ten days of culture and (c) eighteen days of culture.
Mentions: Based on the results of inhibition of F. graminearum growth, twenty bacterial strains were tested. After four days of incubation, there were some mycelia growing in the control wheat kernels with deionized water instead of the bacterial cell-free culture supernatants, while no mycelia were observed on the wheat kernels with the tested bacterial cell-free culture supernatants in the flasks (Figure 2a). On the tenth day, there were obvious differences in the antagonistic activities of the tested bacterial strains. In the flasks with the culture supernatants of some strains, such as WPP1-1 and HPP8, the inhibition activity was weak; whereas to some strains such as WPP10 and WPS4-1, the mycelia were obviously less than those in the control (Figure 2b). After 18 days’ of incubation, the wheat kernels were all covered with mycelia both in the control and tested flasks, and there were no differences of the amount of mycelia visibly (Figure 2c). The amount of DON produced in wheat kernels was determined by HPLC, and five strains XPP6-1, WPP1-2, WPP9, WPP10 and WPS4-1 with strong antagonistic activity against DON production were confirmed, with strain WPS4-1 showing the highest inhibition rate of 90.30% ± 1.01%, followed by strain WPP9 with an inhibition rate of 88.40% ± 4.41%. Strains WPP1-1 and HPP8 showed little inhibition activity towards the DON production in wheat kernels (Table 1).

Bottom Line: Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens.The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels.As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

View Article: PubMed Central - PubMed

Affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China. shicjwh@126.com.

ABSTRACT
Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

Show MeSH
Related in: MedlinePlus