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Biocontrol of Fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists.

Shi C, Yan P, Li J, Wu H, Li Q, Guan S - Int J Environ Res Public Health (2014)

Bottom Line: Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens.The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels.As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

View Article: PubMed Central - PubMed

Affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China. shicjwh@126.com.

ABSTRACT
Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

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Related in: MedlinePlus

The inhibition of three bacterial culture supernatants with a serial dilution against F. graminearum growth in the tip-culture assay.
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ijerph-11-01094-f001: The inhibition of three bacterial culture supernatants with a serial dilution against F. graminearum growth in the tip-culture assay.

Mentions: The antifungal activity of bacterial extracellular metabolites was studied by tip-culture assay using the cell-free culture supernatants. Almost all the strains, with inhibition rates of more than 40% in the dual-culture plate method, showed significant inhibition of the growth of F. graminearum (Table 1). The cell-free culture supernatants of WPP9, WPP10 and WPS4-1 could completely inhibit the germination of conidia. Furthermore, their culture supernatants with a serial dilution were used in tip-culture assay. The results showed that the inhibition rate was dose-dependent on the dilution multiples (Figure 1). Among them, strain WPS4-1 exhibited strongest inhibitory effect as it still inhibited the mycelia growth by 76.8% ± 0.7% even at 16-fold dilution. At the same time, strain WPP10 also exhibited an inhibition rate of 71.9% ± 0.4% at the same dilution rate.


Biocontrol of Fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists.

Shi C, Yan P, Li J, Wu H, Li Q, Guan S - Int J Environ Res Public Health (2014)

The inhibition of three bacterial culture supernatants with a serial dilution against F. graminearum growth in the tip-culture assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924494&req=5

ijerph-11-01094-f001: The inhibition of three bacterial culture supernatants with a serial dilution against F. graminearum growth in the tip-culture assay.
Mentions: The antifungal activity of bacterial extracellular metabolites was studied by tip-culture assay using the cell-free culture supernatants. Almost all the strains, with inhibition rates of more than 40% in the dual-culture plate method, showed significant inhibition of the growth of F. graminearum (Table 1). The cell-free culture supernatants of WPP9, WPP10 and WPS4-1 could completely inhibit the germination of conidia. Furthermore, their culture supernatants with a serial dilution were used in tip-culture assay. The results showed that the inhibition rate was dose-dependent on the dilution multiples (Figure 1). Among them, strain WPS4-1 exhibited strongest inhibitory effect as it still inhibited the mycelia growth by 76.8% ± 0.7% even at 16-fold dilution. At the same time, strain WPP10 also exhibited an inhibition rate of 71.9% ± 0.4% at the same dilution rate.

Bottom Line: Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens.The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels.As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

View Article: PubMed Central - PubMed

Affiliation: School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China. shicjwh@126.com.

ABSTRACT
Fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. Bacterial strains isolated from peanut shells were investigated for their activities against F. graminearum by dual-culture plate and tip-culture assays. Among them, twenty strains exhibited potent inhibition to the growth of F. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in dual-culture plate assay and 92.70% to 100% in tip-culture assay. Furthermore, eighteen strains reduced the production of deoxynivalenol by 16.69% to 90.30% in the wheat kernels assay. Finally, the strains with the strongest inhibitory activity were identified by morphological, physiological, biochemical methods and also 16S rDNA and gyrA gene analysis as Bacillus amyloliquefaciens. The current study highlights the potential application of antagonistic microorganisms and their metabolites in the prevention of fungal growth and mycotoxin production in wheat kernels. As a biological strategy, it might avoid safety problems and nutrition loss which always caused by physical and chemical strategies.

Show MeSH
Related in: MedlinePlus