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Maize purple plant pigment protects against fluoride-induced oxidative damage of liver and kidney in rats.

Zhang Z, Zhou B, Wang H, Wang F, Song Y, Liu S, Xi S - Int J Environ Res Public Health (2014)

Bottom Line: MPPP significantly attenuated these fluoride-induced pathological changes.The presence of 5 g/kg MPPP in the diet reduced the elevation of MDA levels in blood and liver, and increased the SOD and GSH-Px activities in kidney and GSH level in liver and kidney compared with the fluoride alone treated group (p < 0.05).In addition, MPPP alleviated the decrease of Bcl-2 protein expression and the increase of Bax protein expression induced by fluoride.

View Article: PubMed Central - PubMed

Affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University, No. 92 Bei Er Road, Heping District, Shenyang 110001, China. zhangzhuo618@139.com.

ABSTRACT
Anthocyanins are polyphenols and well known for their biological antioxidative benefits. Maize purple plant pigment (MPPP) extracted and separated from maize purple plant is rich in anthocyanins. In the present study, MPPP was used to alleviate the adverse effects generated by fluoride on liver and kidney in rats. The results showed that the ultrastructure of the liver and kidney in fluoride treated rats displayed shrinkage of nuclear and cell volume, swollen mitochondria and endoplasmic reticulum and vacuols formation in the liver and kidney cells. MPPP significantly attenuated these fluoride-induced pathological changes. The MDA levels in serum and liver tissue of fluoride alone treated group were significantly higher than those of the control group (p < 0.05). The presence of 5 g/kg MPPP in the diet reduced the elevation of MDA levels in blood and liver, and increased the SOD and GSH-Px activities in kidney and GSH level in liver and kidney compared with the fluoride alone treated group (p < 0.05). In addition, MPPP alleviated the decrease of Bcl-2 protein expression and the increase of Bax protein expression induced by fluoride. This study demonstrated the protective role of MPPP against fluoride-induced oxidative stress in liver and kidney of rats.

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The expressions of Bcl-2 and Bax in liver and kidney of rats. (a) Liver was isolated for western blot analysis to determine protein expressions of Bcl-2 and Bax. (b) The expressions of Bcl-2 and Bax in kidney were determined.
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ijerph-11-01020-f003: The expressions of Bcl-2 and Bax in liver and kidney of rats. (a) Liver was isolated for western blot analysis to determine protein expressions of Bcl-2 and Bax. (b) The expressions of Bcl-2 and Bax in kidney were determined.

Mentions: Expressions of Bax and Bcl-2 in liver and kidney were measured by western blot analysis, as shown in Figure 3. The protein expressions of Bax in liver and kidney dramatically increased in fluoride alone-treated rats compares with the control group rats, and decreased in 5 g/kg and 10 g/kg MPPP groups compared with fluoride alone-treated group.


Maize purple plant pigment protects against fluoride-induced oxidative damage of liver and kidney in rats.

Zhang Z, Zhou B, Wang H, Wang F, Song Y, Liu S, Xi S - Int J Environ Res Public Health (2014)

The expressions of Bcl-2 and Bax in liver and kidney of rats. (a) Liver was isolated for western blot analysis to determine protein expressions of Bcl-2 and Bax. (b) The expressions of Bcl-2 and Bax in kidney were determined.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924489&req=5

ijerph-11-01020-f003: The expressions of Bcl-2 and Bax in liver and kidney of rats. (a) Liver was isolated for western blot analysis to determine protein expressions of Bcl-2 and Bax. (b) The expressions of Bcl-2 and Bax in kidney were determined.
Mentions: Expressions of Bax and Bcl-2 in liver and kidney were measured by western blot analysis, as shown in Figure 3. The protein expressions of Bax in liver and kidney dramatically increased in fluoride alone-treated rats compares with the control group rats, and decreased in 5 g/kg and 10 g/kg MPPP groups compared with fluoride alone-treated group.

Bottom Line: MPPP significantly attenuated these fluoride-induced pathological changes.The presence of 5 g/kg MPPP in the diet reduced the elevation of MDA levels in blood and liver, and increased the SOD and GSH-Px activities in kidney and GSH level in liver and kidney compared with the fluoride alone treated group (p < 0.05).In addition, MPPP alleviated the decrease of Bcl-2 protein expression and the increase of Bax protein expression induced by fluoride.

View Article: PubMed Central - PubMed

Affiliation: Department of Occupational and Environmental Health, School of Public Health, China Medical University, No. 92 Bei Er Road, Heping District, Shenyang 110001, China. zhangzhuo618@139.com.

ABSTRACT
Anthocyanins are polyphenols and well known for their biological antioxidative benefits. Maize purple plant pigment (MPPP) extracted and separated from maize purple plant is rich in anthocyanins. In the present study, MPPP was used to alleviate the adverse effects generated by fluoride on liver and kidney in rats. The results showed that the ultrastructure of the liver and kidney in fluoride treated rats displayed shrinkage of nuclear and cell volume, swollen mitochondria and endoplasmic reticulum and vacuols formation in the liver and kidney cells. MPPP significantly attenuated these fluoride-induced pathological changes. The MDA levels in serum and liver tissue of fluoride alone treated group were significantly higher than those of the control group (p < 0.05). The presence of 5 g/kg MPPP in the diet reduced the elevation of MDA levels in blood and liver, and increased the SOD and GSH-Px activities in kidney and GSH level in liver and kidney compared with the fluoride alone treated group (p < 0.05). In addition, MPPP alleviated the decrease of Bcl-2 protein expression and the increase of Bax protein expression induced by fluoride. This study demonstrated the protective role of MPPP against fluoride-induced oxidative stress in liver and kidney of rats.

Show MeSH