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Bioaerosols from a food waste composting plant affect human airway epithelial cell remodeling genes.

Chang MW, Lee CR, Hung HF, Teng KS, Huang H, Chuang CY - Int J Environ Res Public Health (2013)

Bottom Line: The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 10(2) conidia.Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM.It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, No. 101, Sec. 2, Kuang-Fu Rd., Hsinchu City 30013, Taiwan. cychuang@mx.nthu.edu.tw.

ABSTRACT
The composting procedure in food waste plants generates airborne bioaerosols that have the potential to damage human airway epithelial cells. Persistent inflammation and repair responses induce airway remodeling and damage to the respiratory system. This study elucidated the expression changes of airway remodeling genes in human lung mucoepidermoid NCI-H292 cells exposed to bioaerosols from a composting plant. Different types of microorganisms were detectable in the composting plant, using the agar culture method. Real-time polymerase chain reaction was used to quantify the level of Aspergillus fumigatus and the profile of remodeling genes. The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 10(2) conidia. The endotoxins in the field bioaerosols were determined using a limulus amebocyte lysate test. The endotoxin levels depended on the type of particulate matter (PM), with coarse particles (2.5-10 μm) having higher endotoxin levels than did fine particles (0.5-2.5 μm). After exposure to the conditioned medium of field bioaerosol samples, NCI-H292 cells showed increased pro-inflammatory interleukin (IL)-6 release and activated epidermal growth factor receptor (EGFR), transforming growth factor (TGF)-β1 and cyclin-dependent kinase inhibitor 1 (p21 WAF1/CIP1) gene expression, but not of matrix metallopeptidase (MMP)-9. Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM. This suggested that airborne bioaerosols in the composting plant contained endotoxins and microorganisms besides A. fumigatus that cause the inflammatory cytokine secretion and augment the expression of remodeling genes in NCI-H292 cells. It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.

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Related in: MedlinePlus

Layout of sampling pots of the food waste composting plant. The white color rea belongs an open access environment. The composting hall, maintenance, liquid fertilizer area, clean area, office and restaurant are closed building equipped with windows and ventilation system.
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ijerph-11-00337-f001: Layout of sampling pots of the food waste composting plant. The white color rea belongs an open access environment. The composting hall, maintenance, liquid fertilizer area, clean area, office and restaurant are closed building equipped with windows and ventilation system.

Mentions: The layout of sampling locations in the composting plant is shown in Figure 1. The sampling of airborne microorganisms was carried out with a single stage ambient viable microbe sampler (TI-10-890, Tisch Environmental, Inc., Cleves, OH, USA) in the composting hall, the maintenance area and the restaurant of composting plant. The sampler with an aerodynamic cut-off size of 0.65 μm at a flow rate of 28.3 L/min was equipped with various agar media to isolate different groups of microorganisms. Meanwhile, for cytotoxicity assay in particulate matter of the composting hall, a filter cassette mounted with a polycarbonate membrane filter (0.4 μm pore size; SKC. Inc., Eighty Four, PA, USA) was used to collect particles at a flow rate of 4 L/min. The inlet of the one-stage viable sampler was located 1.5 m above the ground [34]. This study executed the 8-h bioaerosol sampling twelve times in the composting hall during the normal work period (8 a.m.–4 p.m.) from March to August.


Bioaerosols from a food waste composting plant affect human airway epithelial cell remodeling genes.

Chang MW, Lee CR, Hung HF, Teng KS, Huang H, Chuang CY - Int J Environ Res Public Health (2013)

Layout of sampling pots of the food waste composting plant. The white color rea belongs an open access environment. The composting hall, maintenance, liquid fertilizer area, clean area, office and restaurant are closed building equipped with windows and ventilation system.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924447&req=5

ijerph-11-00337-f001: Layout of sampling pots of the food waste composting plant. The white color rea belongs an open access environment. The composting hall, maintenance, liquid fertilizer area, clean area, office and restaurant are closed building equipped with windows and ventilation system.
Mentions: The layout of sampling locations in the composting plant is shown in Figure 1. The sampling of airborne microorganisms was carried out with a single stage ambient viable microbe sampler (TI-10-890, Tisch Environmental, Inc., Cleves, OH, USA) in the composting hall, the maintenance area and the restaurant of composting plant. The sampler with an aerodynamic cut-off size of 0.65 μm at a flow rate of 28.3 L/min was equipped with various agar media to isolate different groups of microorganisms. Meanwhile, for cytotoxicity assay in particulate matter of the composting hall, a filter cassette mounted with a polycarbonate membrane filter (0.4 μm pore size; SKC. Inc., Eighty Four, PA, USA) was used to collect particles at a flow rate of 4 L/min. The inlet of the one-stage viable sampler was located 1.5 m above the ground [34]. This study executed the 8-h bioaerosol sampling twelve times in the composting hall during the normal work period (8 a.m.–4 p.m.) from March to August.

Bottom Line: The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 10(2) conidia.Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM.It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, No. 101, Sec. 2, Kuang-Fu Rd., Hsinchu City 30013, Taiwan. cychuang@mx.nthu.edu.tw.

ABSTRACT
The composting procedure in food waste plants generates airborne bioaerosols that have the potential to damage human airway epithelial cells. Persistent inflammation and repair responses induce airway remodeling and damage to the respiratory system. This study elucidated the expression changes of airway remodeling genes in human lung mucoepidermoid NCI-H292 cells exposed to bioaerosols from a composting plant. Different types of microorganisms were detectable in the composting plant, using the agar culture method. Real-time polymerase chain reaction was used to quantify the level of Aspergillus fumigatus and the profile of remodeling genes. The real-time PCR results indicated that the amount of A. fumigatus in the composting hall was less than 10(2) conidia. The endotoxins in the field bioaerosols were determined using a limulus amebocyte lysate test. The endotoxin levels depended on the type of particulate matter (PM), with coarse particles (2.5-10 μm) having higher endotoxin levels than did fine particles (0.5-2.5 μm). After exposure to the conditioned medium of field bioaerosol samples, NCI-H292 cells showed increased pro-inflammatory interleukin (IL)-6 release and activated epidermal growth factor receptor (EGFR), transforming growth factor (TGF)-β1 and cyclin-dependent kinase inhibitor 1 (p21 WAF1/CIP1) gene expression, but not of matrix metallopeptidase (MMP)-9. Airborne endotoxin levels were higher inside the composting hall than they were in other areas, and they were associated with PM. This suggested that airborne bioaerosols in the composting plant contained endotoxins and microorganisms besides A. fumigatus that cause the inflammatory cytokine secretion and augment the expression of remodeling genes in NCI-H292 cells. It is thus necessary to monitor potentially hazardous materials from bioaerosols in food composting plants, which could affect the health of workers.

Show MeSH
Related in: MedlinePlus