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Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang on mast cells and allergic rhinitis.

Jeong KT, Kim SG, Lee J, Park YN, Park HH, Park NY, Kim KJ, Lee H, Lee YJ, Lee E - BMC Complement Altern Med (2014)

Bottom Line: In the OVA-induced AR model, the increased levels of IgE were reduced by EBT.The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea. eklee@ynu.ac.kr.

ABSTRACT

Background: Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.

Methods: The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.

Results: EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.

Conclusions: Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

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Related in: MedlinePlus

Histological analysis of nasal mucosa tissue. Eighteen hours after the OVA challenge, the nasal mucosa tissues were removed and histological analysis was conducted. The nasal cavities were decalcified and fixed. The sections of control (NC; PBS-induced, PC; OVA-induced) mice, and AR mice treated with EBT or Dex were stained with H&E (A, magnification x 200; B, magnification x 200) or PAS (C, magnification x 100).
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Figure 5: Histological analysis of nasal mucosa tissue. Eighteen hours after the OVA challenge, the nasal mucosa tissues were removed and histological analysis was conducted. The nasal cavities were decalcified and fixed. The sections of control (NC; PBS-induced, PC; OVA-induced) mice, and AR mice treated with EBT or Dex were stained with H&E (A, magnification x 200; B, magnification x 200) or PAS (C, magnification x 100).

Mentions: The infiltration of leukocytes and mucus secretion are indexes of AR. We stained the nasal mucosa with H&E and PAS staining solution to examine the inhibitory effect of EBT on histological changes in the OVA-induced AR model. The inflammatory cells (such as mast cells and eosinophils) in the nasal mucosa of OVA-induced mice increased compared to those in control mice. A marked reduction in the infiltration of inflammatory cells was observed in the mucosa from mice that were treated with EBT (Figure 5A). The mucous membrane lining the nostril is shown in Figure 5B. In the OVA-induced mice, histological analysis showed the epithelial disruption and pre-treatment with EBT decreased the damage to the nasal epithelium, especially at a dose of 200 mg/kg. We also examined the effect of EBT on histological changes of the nasal mucosa by PAS staining. The mucus secretion in nasal mucosal tissue was increased in the AR mice compared with the control mice and EBT treatment reduced the mucus secretion (Figure 5C).


Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang on mast cells and allergic rhinitis.

Jeong KT, Kim SG, Lee J, Park YN, Park HH, Park NY, Kim KJ, Lee H, Lee YJ, Lee E - BMC Complement Altern Med (2014)

Histological analysis of nasal mucosa tissue. Eighteen hours after the OVA challenge, the nasal mucosa tissues were removed and histological analysis was conducted. The nasal cavities were decalcified and fixed. The sections of control (NC; PBS-induced, PC; OVA-induced) mice, and AR mice treated with EBT or Dex were stained with H&E (A, magnification x 200; B, magnification x 200) or PAS (C, magnification x 100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924403&req=5

Figure 5: Histological analysis of nasal mucosa tissue. Eighteen hours after the OVA challenge, the nasal mucosa tissues were removed and histological analysis was conducted. The nasal cavities were decalcified and fixed. The sections of control (NC; PBS-induced, PC; OVA-induced) mice, and AR mice treated with EBT or Dex were stained with H&E (A, magnification x 200; B, magnification x 200) or PAS (C, magnification x 100).
Mentions: The infiltration of leukocytes and mucus secretion are indexes of AR. We stained the nasal mucosa with H&E and PAS staining solution to examine the inhibitory effect of EBT on histological changes in the OVA-induced AR model. The inflammatory cells (such as mast cells and eosinophils) in the nasal mucosa of OVA-induced mice increased compared to those in control mice. A marked reduction in the infiltration of inflammatory cells was observed in the mucosa from mice that were treated with EBT (Figure 5A). The mucous membrane lining the nostril is shown in Figure 5B. In the OVA-induced mice, histological analysis showed the epithelial disruption and pre-treatment with EBT decreased the damage to the nasal epithelium, especially at a dose of 200 mg/kg. We also examined the effect of EBT on histological changes of the nasal mucosa by PAS staining. The mucus secretion in nasal mucosal tissue was increased in the AR mice compared with the control mice and EBT treatment reduced the mucus secretion (Figure 5C).

Bottom Line: In the OVA-induced AR model, the increased levels of IgE were reduced by EBT.The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea. eklee@ynu.ac.kr.

ABSTRACT

Background: Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.

Methods: The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.

Results: EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.

Conclusions: Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

Show MeSH
Related in: MedlinePlus