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Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang on mast cells and allergic rhinitis.

Jeong KT, Kim SG, Lee J, Park YN, Park HH, Park NY, Kim KJ, Lee H, Lee YJ, Lee E - BMC Complement Altern Med (2014)

Bottom Line: In the OVA-induced AR model, the increased levels of IgE were reduced by EBT.The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea. eklee@ynu.ac.kr.

ABSTRACT

Background: Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.

Methods: The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.

Results: EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.

Conclusions: Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

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Effect of EBT on cell viability, β-Hex release, LTC4 and PGD2 productions. (A) BMMC were treated with different concentrations of EBT and the cell viability was measured. BMMC were pre-incubated with the indicated concentrations of EBT for 1 hr and stimulated with KL (100 ng/ml) for 15 min. Levels of (B) β-Hex and (D) LTC4 released into the supernatant were determined. (C) BMMC were pre-incubated with 10 μg/mL of aspirin for 2 h to abolish pre-existing COX-1 activity, followed by washing and then stimulation with KL/IL-10/LPS for 7 h. PGD2 released into the supernatant was quantified. *: P < 0.05, **: P < 0.01, and ***: P < 0.001 significantly different from AR mice as determined by a one-way ANOVA.
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Figure 2: Effect of EBT on cell viability, β-Hex release, LTC4 and PGD2 productions. (A) BMMC were treated with different concentrations of EBT and the cell viability was measured. BMMC were pre-incubated with the indicated concentrations of EBT for 1 hr and stimulated with KL (100 ng/ml) for 15 min. Levels of (B) β-Hex and (D) LTC4 released into the supernatant were determined. (C) BMMC were pre-incubated with 10 μg/mL of aspirin for 2 h to abolish pre-existing COX-1 activity, followed by washing and then stimulation with KL/IL-10/LPS for 7 h. PGD2 released into the supernatant was quantified. *: P < 0.05, **: P < 0.01, and ***: P < 0.001 significantly different from AR mice as determined by a one-way ANOVA.

Mentions: The effects of EBT concentration (50, 100, and 200 μg/ml) on cell viability were assessed by an MTS assay and there was no significant change in cell viability observed in the response to these concentrations (Figure 2A). When mast cells are activated through IgE-dependent or IgE-independent ways, they release preformed mediators from their granules and produce newly synthesized eicosanoids, chemokines and cytokines [6,7]. Degranulation was monitored by determining the release of β-Hex because histamine release by activated mast cells parallels the release of β-Hex. As shown in Figure 2B, pre-treatment with EBT resulted in a dose-dependent inhibition of β-Hex release, with an IC50 value of 35.6 μg/ml. Eicosanoids such as PGD2 and LTC4 secreted from activated mast cells have long been implicated in the etiology and manifestation of inflammation and allergic diseases [8]. To assess COX-2 dependent PGD2 generation, mast cells were pre-treated with aspirin to abolish any pre-existing COX-1 activity, and stimulated with the KL/IL-10/LPS. The PGD2 production was dose-dependently inhibited by EBT, with an IC50 value of 7.0 μg/ml (Figure 2C). We also assessed the inhibitory activity of EBT on LTC4 production and showed that EBT consistently inhibited LTC4 generation in a dose-dependent manner, with an IC50 value of 10.9 μg/ml (Figure 2D). These results suggested that EBT has COX-2/5-LOX dual inhibitory activities.


Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang on mast cells and allergic rhinitis.

Jeong KT, Kim SG, Lee J, Park YN, Park HH, Park NY, Kim KJ, Lee H, Lee YJ, Lee E - BMC Complement Altern Med (2014)

Effect of EBT on cell viability, β-Hex release, LTC4 and PGD2 productions. (A) BMMC were treated with different concentrations of EBT and the cell viability was measured. BMMC were pre-incubated with the indicated concentrations of EBT for 1 hr and stimulated with KL (100 ng/ml) for 15 min. Levels of (B) β-Hex and (D) LTC4 released into the supernatant were determined. (C) BMMC were pre-incubated with 10 μg/mL of aspirin for 2 h to abolish pre-existing COX-1 activity, followed by washing and then stimulation with KL/IL-10/LPS for 7 h. PGD2 released into the supernatant was quantified. *: P < 0.05, **: P < 0.01, and ***: P < 0.001 significantly different from AR mice as determined by a one-way ANOVA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924403&req=5

Figure 2: Effect of EBT on cell viability, β-Hex release, LTC4 and PGD2 productions. (A) BMMC were treated with different concentrations of EBT and the cell viability was measured. BMMC were pre-incubated with the indicated concentrations of EBT for 1 hr and stimulated with KL (100 ng/ml) for 15 min. Levels of (B) β-Hex and (D) LTC4 released into the supernatant were determined. (C) BMMC were pre-incubated with 10 μg/mL of aspirin for 2 h to abolish pre-existing COX-1 activity, followed by washing and then stimulation with KL/IL-10/LPS for 7 h. PGD2 released into the supernatant was quantified. *: P < 0.05, **: P < 0.01, and ***: P < 0.001 significantly different from AR mice as determined by a one-way ANOVA.
Mentions: The effects of EBT concentration (50, 100, and 200 μg/ml) on cell viability were assessed by an MTS assay and there was no significant change in cell viability observed in the response to these concentrations (Figure 2A). When mast cells are activated through IgE-dependent or IgE-independent ways, they release preformed mediators from their granules and produce newly synthesized eicosanoids, chemokines and cytokines [6,7]. Degranulation was monitored by determining the release of β-Hex because histamine release by activated mast cells parallels the release of β-Hex. As shown in Figure 2B, pre-treatment with EBT resulted in a dose-dependent inhibition of β-Hex release, with an IC50 value of 35.6 μg/ml. Eicosanoids such as PGD2 and LTC4 secreted from activated mast cells have long been implicated in the etiology and manifestation of inflammation and allergic diseases [8]. To assess COX-2 dependent PGD2 generation, mast cells were pre-treated with aspirin to abolish any pre-existing COX-1 activity, and stimulated with the KL/IL-10/LPS. The PGD2 production was dose-dependently inhibited by EBT, with an IC50 value of 7.0 μg/ml (Figure 2C). We also assessed the inhibitory activity of EBT on LTC4 production and showed that EBT consistently inhibited LTC4 generation in a dose-dependent manner, with an IC50 value of 10.9 μg/ml (Figure 2D). These results suggested that EBT has COX-2/5-LOX dual inhibitory activities.

Bottom Line: In the OVA-induced AR model, the increased levels of IgE were reduced by EBT.The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea. eklee@ynu.ac.kr.

ABSTRACT

Background: Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.

Methods: The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.

Results: EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.

Conclusions: Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.

Show MeSH
Related in: MedlinePlus