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Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls.

Yue XP, Dechow C, Chang TC, DeJarnette JM, Marshall CE, Lei CZ, Liu WS - BMC Genomics (2014)

Bottom Line: Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284).The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution.The CN of both gene families varies significantly among individuals and cattle breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. leichuzhao1118@126.com.

ABSTRACT

Background: Recent transcriptomic analysis of the bovine Y chromosome revealed at least six multi-copy protein coding gene families, including TSPY, HSFY and ZNF280BY, on the male-specific region (MSY). Previous studies indicated that the copy number variations (CNVs) of the human and bovine TSPY were associated with male fertility in men and cattle. However, the relationship between CNVs of the bovine Y-linked HSFY and ZNF280BY gene families and bull fertility has not been investigated.

Results: We investigated the copy number (CN) of the bovine HSFY and ZNF280BY in a total of 460 bulls from 15 breeds using a quantitative PCR approach. We observed CNVs for both gene families within and between cattle breeds. The median copy number (MCN) of HSFY among all bulls was 197, ranging from 21 to 308. The MCN of ZNF280BY was 236, varying from 28 to 380. Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284). In addition, the CN of ZNF280BY was positively correlated to that of HSFY on the BTAY. Association analysis revealed that the CNVs of both HSFY and ZNF280BY were correlated negatively with testis size, while positively with sire conception rate.

Conclusion: The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution. The CN of both gene families varies significantly among individuals and cattle breeds. These variations were associated with testis size and bull fertility in Holstein, suggesting that the CNVs of HSFY and ZNF280BY may serve as valuable makers for male fertility selection in cattle.

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Correlations between the CNV of HSFY and ZNF280BY and male reproductive traits in Holsteins. The CNV of HSFY was (A) negatively associated with RLSC (r = -0.249, P = 0.008) and (B) positively associated with SCR (r = 294, P = 0.021). The ZNF280BY CNV was (C) negatively associated with RLSC (r = -0.174, P = 0.055) and (D) positively associated with SCR (r = 0.339, P = 0.008). Each circle represents one individual. The animal that has the lowest CN of HSFY and ZNF280BY was marked with a red square.
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Figure 4: Correlations between the CNV of HSFY and ZNF280BY and male reproductive traits in Holsteins. The CNV of HSFY was (A) negatively associated with RLSC (r = -0.249, P = 0.008) and (B) positively associated with SCR (r = 294, P = 0.021). The ZNF280BY CNV was (C) negatively associated with RLSC (r = -0.174, P = 0.055) and (D) positively associated with SCR (r = 0.339, P = 0.008). Each circle represents one individual. The animal that has the lowest CN of HSFY and ZNF280BY was marked with a red square.

Mentions: We performed the association analysis of CNVs of HSFY and ZNF280BY and their logarithm (base 10) transformation data with the three types of reproductive traits from 257 Holstein bulls, including testis size (RLSC and SC), semen quality (PTM, IM, PNS and PIA) and male fertility (SCR, NRR and RBE). The association analysis revealed that the RLSC was negatively associated with CNV (r = -0.249, P = 0.008) and Log10CNV of HSFY (r = -0.267, P = 0.004) (Table 6), and SC was negatively associated with Log10CNV of HSFY (r = -0.202, P = 0.025), while tended to be negatively associated with CNV of HSFY (r = -0.169, P = 0.073). For the ZNF280BY, RLSC is significantly associated with log10CNV of ZNF280BY (r = -0.239, P = 0.025), and tend to be associated with its CNV (r = -0.174, P = 0.055). SC was neither associated with CNV nor log10CNV of ZNF280BY (Table 6). These results suggested that a lower CN of HSFY and ZNF280BY is associated with a larger testis size (Figure 4). The results also demonstrated that the CNVs or Log10CNVs of HSFY and ZNF280BY were positively correlated to SCR, suggesting that a higher CN of HSFY and ZNF280BY is associated with a higher SCR (Figure 4). Furthermore, we investigated the relationship between reproductive traits and the sum of HSFY and ZNF280BY CNV (sCNV) and Log10sCNV. We found that sCNV and Log10sCNV of HSFY and ZNF280BY were negatively associated with RLSC, whereas positively associated with SCR. The remaining traits were not significantly associated with either sCNV or Log10sCNV (Table 6). Interestingly, the Holstein bull (mentioned above) who had the lowest copy number in both HSFY and ZNF280BY gene families had the largest RLSC among all animals tested, strongly supporting our association results that a lower CN of the two Y-linked genes is associated with a larger testis.


Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls.

Yue XP, Dechow C, Chang TC, DeJarnette JM, Marshall CE, Lei CZ, Liu WS - BMC Genomics (2014)

Correlations between the CNV of HSFY and ZNF280BY and male reproductive traits in Holsteins. The CNV of HSFY was (A) negatively associated with RLSC (r = -0.249, P = 0.008) and (B) positively associated with SCR (r = 294, P = 0.021). The ZNF280BY CNV was (C) negatively associated with RLSC (r = -0.174, P = 0.055) and (D) positively associated with SCR (r = 0.339, P = 0.008). Each circle represents one individual. The animal that has the lowest CN of HSFY and ZNF280BY was marked with a red square.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924399&req=5

Figure 4: Correlations between the CNV of HSFY and ZNF280BY and male reproductive traits in Holsteins. The CNV of HSFY was (A) negatively associated with RLSC (r = -0.249, P = 0.008) and (B) positively associated with SCR (r = 294, P = 0.021). The ZNF280BY CNV was (C) negatively associated with RLSC (r = -0.174, P = 0.055) and (D) positively associated with SCR (r = 0.339, P = 0.008). Each circle represents one individual. The animal that has the lowest CN of HSFY and ZNF280BY was marked with a red square.
Mentions: We performed the association analysis of CNVs of HSFY and ZNF280BY and their logarithm (base 10) transformation data with the three types of reproductive traits from 257 Holstein bulls, including testis size (RLSC and SC), semen quality (PTM, IM, PNS and PIA) and male fertility (SCR, NRR and RBE). The association analysis revealed that the RLSC was negatively associated with CNV (r = -0.249, P = 0.008) and Log10CNV of HSFY (r = -0.267, P = 0.004) (Table 6), and SC was negatively associated with Log10CNV of HSFY (r = -0.202, P = 0.025), while tended to be negatively associated with CNV of HSFY (r = -0.169, P = 0.073). For the ZNF280BY, RLSC is significantly associated with log10CNV of ZNF280BY (r = -0.239, P = 0.025), and tend to be associated with its CNV (r = -0.174, P = 0.055). SC was neither associated with CNV nor log10CNV of ZNF280BY (Table 6). These results suggested that a lower CN of HSFY and ZNF280BY is associated with a larger testis size (Figure 4). The results also demonstrated that the CNVs or Log10CNVs of HSFY and ZNF280BY were positively correlated to SCR, suggesting that a higher CN of HSFY and ZNF280BY is associated with a higher SCR (Figure 4). Furthermore, we investigated the relationship between reproductive traits and the sum of HSFY and ZNF280BY CNV (sCNV) and Log10sCNV. We found that sCNV and Log10sCNV of HSFY and ZNF280BY were negatively associated with RLSC, whereas positively associated with SCR. The remaining traits were not significantly associated with either sCNV or Log10sCNV (Table 6). Interestingly, the Holstein bull (mentioned above) who had the lowest copy number in both HSFY and ZNF280BY gene families had the largest RLSC among all animals tested, strongly supporting our association results that a lower CN of the two Y-linked genes is associated with a larger testis.

Bottom Line: Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284).The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution.The CN of both gene families varies significantly among individuals and cattle breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. leichuzhao1118@126.com.

ABSTRACT

Background: Recent transcriptomic analysis of the bovine Y chromosome revealed at least six multi-copy protein coding gene families, including TSPY, HSFY and ZNF280BY, on the male-specific region (MSY). Previous studies indicated that the copy number variations (CNVs) of the human and bovine TSPY were associated with male fertility in men and cattle. However, the relationship between CNVs of the bovine Y-linked HSFY and ZNF280BY gene families and bull fertility has not been investigated.

Results: We investigated the copy number (CN) of the bovine HSFY and ZNF280BY in a total of 460 bulls from 15 breeds using a quantitative PCR approach. We observed CNVs for both gene families within and between cattle breeds. The median copy number (MCN) of HSFY among all bulls was 197, ranging from 21 to 308. The MCN of ZNF280BY was 236, varying from 28 to 380. Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284). In addition, the CN of ZNF280BY was positively correlated to that of HSFY on the BTAY. Association analysis revealed that the CNVs of both HSFY and ZNF280BY were correlated negatively with testis size, while positively with sire conception rate.

Conclusion: The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution. The CN of both gene families varies significantly among individuals and cattle breeds. These variations were associated with testis size and bull fertility in Holstein, suggesting that the CNVs of HSFY and ZNF280BY may serve as valuable makers for male fertility selection in cattle.

Show MeSH
Related in: MedlinePlus