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Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls.

Yue XP, Dechow C, Chang TC, DeJarnette JM, Marshall CE, Lei CZ, Liu WS - BMC Genomics (2014)

Bottom Line: Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284).The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution.The CN of both gene families varies significantly among individuals and cattle breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. leichuzhao1118@126.com.

ABSTRACT

Background: Recent transcriptomic analysis of the bovine Y chromosome revealed at least six multi-copy protein coding gene families, including TSPY, HSFY and ZNF280BY, on the male-specific region (MSY). Previous studies indicated that the copy number variations (CNVs) of the human and bovine TSPY were associated with male fertility in men and cattle. However, the relationship between CNVs of the bovine Y-linked HSFY and ZNF280BY gene families and bull fertility has not been investigated.

Results: We investigated the copy number (CN) of the bovine HSFY and ZNF280BY in a total of 460 bulls from 15 breeds using a quantitative PCR approach. We observed CNVs for both gene families within and between cattle breeds. The median copy number (MCN) of HSFY among all bulls was 197, ranging from 21 to 308. The MCN of ZNF280BY was 236, varying from 28 to 380. Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284). In addition, the CN of ZNF280BY was positively correlated to that of HSFY on the BTAY. Association analysis revealed that the CNVs of both HSFY and ZNF280BY were correlated negatively with testis size, while positively with sire conception rate.

Conclusion: The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution. The CN of both gene families varies significantly among individuals and cattle breeds. These variations were associated with testis size and bull fertility in Holstein, suggesting that the CNVs of HSFY and ZNF280BY may serve as valuable makers for male fertility selection in cattle.

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Gel electrophesis of PCR products of the bovine HSFY, ZNF280BY and DDX3Y. The primers of these three Y-linked genes amplified male specific bands with expected fragment size labeled above the band. Marker: 1 kb DNA ladder; ♂: the Hereford bull L1 Domino 99375 genomic DNA; ♀: female cattle genomic DNA; -: negative control (distilled water).
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Figure 1: Gel electrophesis of PCR products of the bovine HSFY, ZNF280BY and DDX3Y. The primers of these three Y-linked genes amplified male specific bands with expected fragment size labeled above the band. Marker: 1 kb DNA ladder; ♂: the Hereford bull L1 Domino 99375 genomic DNA; ♀: female cattle genomic DNA; -: negative control (distilled water).

Mentions: In order to validate the male specificity of primers used in this study, a routine PCR was run. The results demonstrated that every primer pair for the target genes, HSFY and ZNF280BY, or the single copy reference gene, DDX3Y, amplified a male-specific band with the expected fragment size, confirming that the designed primers are male-specific and can be used for the qPCR analysis in this study (Figure 1).


Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls.

Yue XP, Dechow C, Chang TC, DeJarnette JM, Marshall CE, Lei CZ, Liu WS - BMC Genomics (2014)

Gel electrophesis of PCR products of the bovine HSFY, ZNF280BY and DDX3Y. The primers of these three Y-linked genes amplified male specific bands with expected fragment size labeled above the band. Marker: 1 kb DNA ladder; ♂: the Hereford bull L1 Domino 99375 genomic DNA; ♀: female cattle genomic DNA; -: negative control (distilled water).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924399&req=5

Figure 1: Gel electrophesis of PCR products of the bovine HSFY, ZNF280BY and DDX3Y. The primers of these three Y-linked genes amplified male specific bands with expected fragment size labeled above the band. Marker: 1 kb DNA ladder; ♂: the Hereford bull L1 Domino 99375 genomic DNA; ♀: female cattle genomic DNA; -: negative control (distilled water).
Mentions: In order to validate the male specificity of primers used in this study, a routine PCR was run. The results demonstrated that every primer pair for the target genes, HSFY and ZNF280BY, or the single copy reference gene, DDX3Y, amplified a male-specific band with the expected fragment size, confirming that the designed primers are male-specific and can be used for the qPCR analysis in this study (Figure 1).

Bottom Line: Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284).The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution.The CN of both gene families varies significantly among individuals and cattle breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. leichuzhao1118@126.com.

ABSTRACT

Background: Recent transcriptomic analysis of the bovine Y chromosome revealed at least six multi-copy protein coding gene families, including TSPY, HSFY and ZNF280BY, on the male-specific region (MSY). Previous studies indicated that the copy number variations (CNVs) of the human and bovine TSPY were associated with male fertility in men and cattle. However, the relationship between CNVs of the bovine Y-linked HSFY and ZNF280BY gene families and bull fertility has not been investigated.

Results: We investigated the copy number (CN) of the bovine HSFY and ZNF280BY in a total of 460 bulls from 15 breeds using a quantitative PCR approach. We observed CNVs for both gene families within and between cattle breeds. The median copy number (MCN) of HSFY among all bulls was 197, ranging from 21 to 308. The MCN of ZNF280BY was 236, varying from 28 to 380. Furthermore, bulls in the Bos taurus (BTA) lineage had a significantly higher MCN (202) of HSFY than bulls in the Bos indicus (BIN) lineage (178), while taurine bulls had a significantly lower MCN (231) of ZNF280BY than indicine bulls (284). In addition, the CN of ZNF280BY was positively correlated to that of HSFY on the BTAY. Association analysis revealed that the CNVs of both HSFY and ZNF280BY were correlated negatively with testis size, while positively with sire conception rate.

Conclusion: The bovine HSFY and ZNF280BY gene families have extensively expanded on the Y chromosome during evolution. The CN of both gene families varies significantly among individuals and cattle breeds. These variations were associated with testis size and bull fertility in Holstein, suggesting that the CNVs of HSFY and ZNF280BY may serve as valuable makers for male fertility selection in cattle.

Show MeSH
Related in: MedlinePlus