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Lower doses of isoflurane treatment has no beneficial effects in a rat model of intracerebral hemorrhage.

Esposito E, Mandeville ET, Lo EH - BMC Neurosci (2013)

Bottom Line: Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations.Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used.Plasma MMP levels did not change.

View Article: PubMed Central - HTML - PubMed

Affiliation: Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, USA. elga.esposito@gmail.com.

ABSTRACT

Background: Intracerebral hemorrhage is a subtype of stroke that has a poor prognosis without an adequate therapy. Recently, the use of anesthetics such as isoflurane has been shown to be protective after cerebral ischemia. However, the potential therapeutic effect of isoflurane after intracerebral hemorrhage (ICH) has not been fully explored.

Results: In this study, male Sprague-Dawley rats (SD) were subjected to ICH and randomized into controls and 1.2% or 1.5% isoflurane posttreatment groups. Brain water content, neurological outcomes and matrix metalloproteinase-2 and -9 (MMP2-MMP9) plasma levels were quantified at 24 hours. Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations. Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used. Plasma MMP levels did not change.

Conclusion: Our data suggested that there is no neuroprotection after isoflurane posttreatment in a rat model of ICH.

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Zymographic detection of MMP-2 and MMP-9 in plasma control and isoflurane groups. (A) Representative gelatin zymography. (B) Densitometric quantization (mean ± S.D.) detected no differences in MMP-2 and MMP-9 plasma levels between the 1.2% and 1.5% isofurane groups vs the control group.
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Figure 3: Zymographic detection of MMP-2 and MMP-9 in plasma control and isoflurane groups. (A) Representative gelatin zymography. (B) Densitometric quantization (mean ± S.D.) detected no differences in MMP-2 and MMP-9 plasma levels between the 1.2% and 1.5% isofurane groups vs the control group.

Mentions: Gel zymography from plasma of rats post-treated with 1.2 or 1.5% isoflurane did not reveal any changes in matrix metalloproteinases MMP-2 or MMP-9 levels compared with control rats at 24 hrs after ICH (MMP9: control group, 100% ± 15.5; 1.2% isoflurane group, 106.86 ± 28.2; 1.5% isoflurane group, 92.14 ± 15.1. P = 0.539. MMP2: control group, 100% ± 15.1; 1.2% isoflurane group, 91.94 ± 12.3; 1.5% isoflurane group, 103.40 ± 7.6. P = 0.223) (Figure 3A,B).


Lower doses of isoflurane treatment has no beneficial effects in a rat model of intracerebral hemorrhage.

Esposito E, Mandeville ET, Lo EH - BMC Neurosci (2013)

Zymographic detection of MMP-2 and MMP-9 in plasma control and isoflurane groups. (A) Representative gelatin zymography. (B) Densitometric quantization (mean ± S.D.) detected no differences in MMP-2 and MMP-9 plasma levels between the 1.2% and 1.5% isofurane groups vs the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924354&req=5

Figure 3: Zymographic detection of MMP-2 and MMP-9 in plasma control and isoflurane groups. (A) Representative gelatin zymography. (B) Densitometric quantization (mean ± S.D.) detected no differences in MMP-2 and MMP-9 plasma levels between the 1.2% and 1.5% isofurane groups vs the control group.
Mentions: Gel zymography from plasma of rats post-treated with 1.2 or 1.5% isoflurane did not reveal any changes in matrix metalloproteinases MMP-2 or MMP-9 levels compared with control rats at 24 hrs after ICH (MMP9: control group, 100% ± 15.5; 1.2% isoflurane group, 106.86 ± 28.2; 1.5% isoflurane group, 92.14 ± 15.1. P = 0.539. MMP2: control group, 100% ± 15.1; 1.2% isoflurane group, 91.94 ± 12.3; 1.5% isoflurane group, 103.40 ± 7.6. P = 0.223) (Figure 3A,B).

Bottom Line: Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations.Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used.Plasma MMP levels did not change.

View Article: PubMed Central - HTML - PubMed

Affiliation: Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, USA. elga.esposito@gmail.com.

ABSTRACT

Background: Intracerebral hemorrhage is a subtype of stroke that has a poor prognosis without an adequate therapy. Recently, the use of anesthetics such as isoflurane has been shown to be protective after cerebral ischemia. However, the potential therapeutic effect of isoflurane after intracerebral hemorrhage (ICH) has not been fully explored.

Results: In this study, male Sprague-Dawley rats (SD) were subjected to ICH and randomized into controls and 1.2% or 1.5% isoflurane posttreatment groups. Brain water content, neurological outcomes and matrix metalloproteinase-2 and -9 (MMP2-MMP9) plasma levels were quantified at 24 hours. Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations. Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used. Plasma MMP levels did not change.

Conclusion: Our data suggested that there is no neuroprotection after isoflurane posttreatment in a rat model of ICH.

Show MeSH
Related in: MedlinePlus