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Lower doses of isoflurane treatment has no beneficial effects in a rat model of intracerebral hemorrhage.

Esposito E, Mandeville ET, Lo EH - BMC Neurosci (2013)

Bottom Line: Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations.Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used.Plasma MMP levels did not change.

View Article: PubMed Central - HTML - PubMed

Affiliation: Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, USA. elga.esposito@gmail.com.

ABSTRACT

Background: Intracerebral hemorrhage is a subtype of stroke that has a poor prognosis without an adequate therapy. Recently, the use of anesthetics such as isoflurane has been shown to be protective after cerebral ischemia. However, the potential therapeutic effect of isoflurane after intracerebral hemorrhage (ICH) has not been fully explored.

Results: In this study, male Sprague-Dawley rats (SD) were subjected to ICH and randomized into controls and 1.2% or 1.5% isoflurane posttreatment groups. Brain water content, neurological outcomes and matrix metalloproteinase-2 and -9 (MMP2-MMP9) plasma levels were quantified at 24 hours. Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations. Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used. Plasma MMP levels did not change.

Conclusion: Our data suggested that there is no neuroprotection after isoflurane posttreatment in a rat model of ICH.

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Related in: MedlinePlus

Neurological outcomes were quantified at 24 hours in 1.2% and 1.5% isoflurane group compared with untreated controls. There were statistically significant differences between any of the treatment groups for (A) the forelimb placement test, (B) body swing test, and (C) neuroscore. All values depicted as mean ± S.D.
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Figure 2: Neurological outcomes were quantified at 24 hours in 1.2% and 1.5% isoflurane group compared with untreated controls. There were statistically significant differences between any of the treatment groups for (A) the forelimb placement test, (B) body swing test, and (C) neuroscore. All values depicted as mean ± S.D.

Mentions: Consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used (Figure 2A,B,C). In the forelimb placement test, 1.2% and 1.5% isoflurane had no effect on the outcomes (control ipsilateral group, 5.43 ± 1.8; 1.2% isoflurane ipsilateral group, 6.33 ± 1.15; 1.5% isoflurane ipsilateral group, 7.1 ± 1.15. P = 0.096). Both 1.2% and 1.5% isoflurane posttreatment also had no detectable effect on the body-swing test (control group, 22.9 ± 7.2; 1.2% isoflurane group, 26.33 ± 3.2; 1.5% isoflurane group, 20.4 ± 10.6. P = 0.368). Finally, isoflurane did not affect neuroscore outcomes (control ipsilateral group, 1.76 ± 1.0; 1.2% isoflurane ipsilateral group, 1.5 ± 0.7; 1.5% isoflurane ipsilateral group, 1.8 ± 0.8. P = 0.773) or rates of mortality.


Lower doses of isoflurane treatment has no beneficial effects in a rat model of intracerebral hemorrhage.

Esposito E, Mandeville ET, Lo EH - BMC Neurosci (2013)

Neurological outcomes were quantified at 24 hours in 1.2% and 1.5% isoflurane group compared with untreated controls. There were statistically significant differences between any of the treatment groups for (A) the forelimb placement test, (B) body swing test, and (C) neuroscore. All values depicted as mean ± S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924354&req=5

Figure 2: Neurological outcomes were quantified at 24 hours in 1.2% and 1.5% isoflurane group compared with untreated controls. There were statistically significant differences between any of the treatment groups for (A) the forelimb placement test, (B) body swing test, and (C) neuroscore. All values depicted as mean ± S.D.
Mentions: Consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used (Figure 2A,B,C). In the forelimb placement test, 1.2% and 1.5% isoflurane had no effect on the outcomes (control ipsilateral group, 5.43 ± 1.8; 1.2% isoflurane ipsilateral group, 6.33 ± 1.15; 1.5% isoflurane ipsilateral group, 7.1 ± 1.15. P = 0.096). Both 1.2% and 1.5% isoflurane posttreatment also had no detectable effect on the body-swing test (control group, 22.9 ± 7.2; 1.2% isoflurane group, 26.33 ± 3.2; 1.5% isoflurane group, 20.4 ± 10.6. P = 0.368). Finally, isoflurane did not affect neuroscore outcomes (control ipsilateral group, 1.76 ± 1.0; 1.2% isoflurane ipsilateral group, 1.5 ± 0.7; 1.5% isoflurane ipsilateral group, 1.8 ± 0.8. P = 0.773) or rates of mortality.

Bottom Line: Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations.Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used.Plasma MMP levels did not change.

View Article: PubMed Central - HTML - PubMed

Affiliation: Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, USA. elga.esposito@gmail.com.

ABSTRACT

Background: Intracerebral hemorrhage is a subtype of stroke that has a poor prognosis without an adequate therapy. Recently, the use of anesthetics such as isoflurane has been shown to be protective after cerebral ischemia. However, the potential therapeutic effect of isoflurane after intracerebral hemorrhage (ICH) has not been fully explored.

Results: In this study, male Sprague-Dawley rats (SD) were subjected to ICH and randomized into controls and 1.2% or 1.5% isoflurane posttreatment groups. Brain water content, neurological outcomes and matrix metalloproteinase-2 and -9 (MMP2-MMP9) plasma levels were quantified at 24 hours. Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations. Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used. Plasma MMP levels did not change.

Conclusion: Our data suggested that there is no neuroprotection after isoflurane posttreatment in a rat model of ICH.

Show MeSH
Related in: MedlinePlus