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Lower doses of isoflurane treatment has no beneficial effects in a rat model of intracerebral hemorrhage.

Esposito E, Mandeville ET, Lo EH - BMC Neurosci (2013)

Bottom Line: Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations.Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used.Plasma MMP levels did not change.

View Article: PubMed Central - HTML - PubMed

Affiliation: Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, USA. elga.esposito@gmail.com.

ABSTRACT

Background: Intracerebral hemorrhage is a subtype of stroke that has a poor prognosis without an adequate therapy. Recently, the use of anesthetics such as isoflurane has been shown to be protective after cerebral ischemia. However, the potential therapeutic effect of isoflurane after intracerebral hemorrhage (ICH) has not been fully explored.

Results: In this study, male Sprague-Dawley rats (SD) were subjected to ICH and randomized into controls and 1.2% or 1.5% isoflurane posttreatment groups. Brain water content, neurological outcomes and matrix metalloproteinase-2 and -9 (MMP2-MMP9) plasma levels were quantified at 24 hours. Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations. Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used. Plasma MMP levels did not change.

Conclusion: Our data suggested that there is no neuroprotection after isoflurane posttreatment in a rat model of ICH.

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Quantization of hemorrhagic edema after 24 hours. Brain water content in the ipsilateral hemisphere was significantly greater than the contralateral hemisphere in both control and isoflurane groups *P < 0.05. However, ipsilateral water content was not affected by 1.2% and1.5% isoflurane. All values depicted as mean ± S.D.
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Figure 1: Quantization of hemorrhagic edema after 24 hours. Brain water content in the ipsilateral hemisphere was significantly greater than the contralateral hemisphere in both control and isoflurane groups *P < 0.05. However, ipsilateral water content was not affected by 1.2% and1.5% isoflurane. All values depicted as mean ± S.D.

Mentions: As expected, this standard model of collagenase injections resulted in reproducible intrastriatal hematomas. There were no statistically significant differences in hematoma volumes in controls vs 1.2% or 1.5% isoflurane treatment groups. Accordingly, brain edema developed in all groups - brain water content in the ipsilateral hemisphere was significantly greater than the contralateral hemisphere (control group: ipsilateral, 81.5% ± 0.8% versus contralateral, 78.9% ± 0.4% P < 0.05; 1.2% isoflurane group: ipsilateral, 81.6% ± 0.2% versus contralateral, 78.8% ± 0.6% P < 0.05; 1.5% isoflurane group: ipsilateral, 81.3% ± 0.4% versus contralateral, 78.7% ± 0.3% P < 0.05). However, isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations (P = 0.653) (Figure 1).


Lower doses of isoflurane treatment has no beneficial effects in a rat model of intracerebral hemorrhage.

Esposito E, Mandeville ET, Lo EH - BMC Neurosci (2013)

Quantization of hemorrhagic edema after 24 hours. Brain water content in the ipsilateral hemisphere was significantly greater than the contralateral hemisphere in both control and isoflurane groups *P < 0.05. However, ipsilateral water content was not affected by 1.2% and1.5% isoflurane. All values depicted as mean ± S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924354&req=5

Figure 1: Quantization of hemorrhagic edema after 24 hours. Brain water content in the ipsilateral hemisphere was significantly greater than the contralateral hemisphere in both control and isoflurane groups *P < 0.05. However, ipsilateral water content was not affected by 1.2% and1.5% isoflurane. All values depicted as mean ± S.D.
Mentions: As expected, this standard model of collagenase injections resulted in reproducible intrastriatal hematomas. There were no statistically significant differences in hematoma volumes in controls vs 1.2% or 1.5% isoflurane treatment groups. Accordingly, brain edema developed in all groups - brain water content in the ipsilateral hemisphere was significantly greater than the contralateral hemisphere (control group: ipsilateral, 81.5% ± 0.8% versus contralateral, 78.9% ± 0.4% P < 0.05; 1.2% isoflurane group: ipsilateral, 81.6% ± 0.2% versus contralateral, 78.8% ± 0.6% P < 0.05; 1.5% isoflurane group: ipsilateral, 81.3% ± 0.4% versus contralateral, 78.7% ± 0.3% P < 0.05). However, isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations (P = 0.653) (Figure 1).

Bottom Line: Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations.Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used.Plasma MMP levels did not change.

View Article: PubMed Central - HTML - PubMed

Affiliation: Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, USA. elga.esposito@gmail.com.

ABSTRACT

Background: Intracerebral hemorrhage is a subtype of stroke that has a poor prognosis without an adequate therapy. Recently, the use of anesthetics such as isoflurane has been shown to be protective after cerebral ischemia. However, the potential therapeutic effect of isoflurane after intracerebral hemorrhage (ICH) has not been fully explored.

Results: In this study, male Sprague-Dawley rats (SD) were subjected to ICH and randomized into controls and 1.2% or 1.5% isoflurane posttreatment groups. Brain water content, neurological outcomes and matrix metalloproteinase-2 and -9 (MMP2-MMP9) plasma levels were quantified at 24 hours. Isoflurane treatment did not reduce brain edema compared with controls in any of the applied isoflurane concentrations. Moreover, consistent with this lack of effect on brain edema, isoflurane posttreatment did not affect neurological outcomes in any of the tests used. Plasma MMP levels did not change.

Conclusion: Our data suggested that there is no neuroprotection after isoflurane posttreatment in a rat model of ICH.

Show MeSH
Related in: MedlinePlus