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Transcriptomic dissection of sexual differences in Bemisia tabaci, an invasive agricultural pest worldwide.

Xie W, Guo L, Jiao X, Yang N, Yang X, Wu Q, Wang S, Zhou X, Zhang Y - Sci Rep (2014)

Bottom Line: Gender-based RNAseq data produced 27 Gb reads, and subsequent de novo assembly generated 93,948 transcripts with a N50 of 1,853 bp.A total of 1,351 differentially expressed genes were identified between male and female B. tabaci, and majority of them were female-biased.Pathway and GO enrichment experiments exhibited a gender-specific expression, including enriched translation in females, and enhanced structural constituent of cuticle in male whiteflies.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China [2].

ABSTRACT
Sex difference involving chromosomes and gene expression has been extensively documented. In this study, the gender difference in the sweetpotato whitefly Bemisia tabaci was investigated using Illumina-based transcriptomic analysis. Gender-based RNAseq data produced 27 Gb reads, and subsequent de novo assembly generated 93,948 transcripts with a N50 of 1,853 bp. A total of 1,351 differentially expressed genes were identified between male and female B. tabaci, and majority of them were female-biased. Pathway and GO enrichment experiments exhibited a gender-specific expression, including enriched translation in females, and enhanced structural constituent of cuticle in male whiteflies. In addition, a putative transformer2 gene (tra2) was cloned, and the structural feature and expression profile of tra2 were investigated. Sexually dimorphic transcriptome is an uncharted territory for the agricultural insect pests. Molecular understanding of sex determination in B. tabaci, an emerging invasive insect pest worldwide, will provide potential molecular target(s) for genetic pest control alternatives.

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Related in: MedlinePlus

Volcano plot illustrating B. tabaci gender differences at transcription level.The x-axis represents the relative expression of transcript for female and male B. tabaci, respectively. The color-shaded regions depict significantly female-biased genes [red, Log2(fold change) > 1, q-value < 0.05], and male-biased genes [blue, Log2(fold change) < −1, q-value < 0.05] among cotton (A), cabbage (B), cucumber (C), and tomato (D).
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f3: Volcano plot illustrating B. tabaci gender differences at transcription level.The x-axis represents the relative expression of transcript for female and male B. tabaci, respectively. The color-shaded regions depict significantly female-biased genes [red, Log2(fold change) > 1, q-value < 0.05], and male-biased genes [blue, Log2(fold change) < −1, q-value < 0.05] among cotton (A), cabbage (B), cucumber (C), and tomato (D).

Mentions: To specifically identify sex-biased genes that might also affect the sex difference process, we performed a series of expression profiling to examine gene activity changes between sexes. By comparing female to male, differentially expressed transcripts (DETs) (q-value < 0.05 and log2(Fold change) > 1or log2(Fold change) < −1) were generated in four host strains (Table S4). This resulted in 8,434 differentially expressed transcripts between Caf and Cam, 7817 between Cuf and Cum, 6967 between Tof and Tom and 5151 between Cof and Com (Table S4). These results suggested that majority of the differentially expressed transcripts between sexes were female-biased. To reduce the DETs between female and male whiteflies, intersection elements of female (Figure 2A) and male (Figure 2B) specific genes were determined. Among them, 1070 female and 281 male specific genes were annotated (Table S1 and S2). The analysis of the transcriptome dynamics was shown in Figure 3. Both the female and male biased genes were enriched with members of distinct KEGG pathways and GO categories. Pathway enrichment tests revealed that pyrimidine metabolism, ribosome, mRNA surveillance pathway, ribosome biogenesis in eukaryotes, RNA polymerase, RNA degradation, DNA replication and RNA transport were enriched in female whitely. In the case of male whitefly, starch and phototransduction, lysosome, nitrogen metabolism, glycerophospholipid metabolism and phagosome were enriched (Figure S1 and S2). The enriched GO analysis for female and male biased genes were shown in Figure S3, which indicated that many enriched GO categories in female included only extracellular region while structural constituent of cuticle and hormone activity were predominant in male (Figure S3).


Transcriptomic dissection of sexual differences in Bemisia tabaci, an invasive agricultural pest worldwide.

Xie W, Guo L, Jiao X, Yang N, Yang X, Wu Q, Wang S, Zhou X, Zhang Y - Sci Rep (2014)

Volcano plot illustrating B. tabaci gender differences at transcription level.The x-axis represents the relative expression of transcript for female and male B. tabaci, respectively. The color-shaded regions depict significantly female-biased genes [red, Log2(fold change) > 1, q-value < 0.05], and male-biased genes [blue, Log2(fold change) < −1, q-value < 0.05] among cotton (A), cabbage (B), cucumber (C), and tomato (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924218&req=5

f3: Volcano plot illustrating B. tabaci gender differences at transcription level.The x-axis represents the relative expression of transcript for female and male B. tabaci, respectively. The color-shaded regions depict significantly female-biased genes [red, Log2(fold change) > 1, q-value < 0.05], and male-biased genes [blue, Log2(fold change) < −1, q-value < 0.05] among cotton (A), cabbage (B), cucumber (C), and tomato (D).
Mentions: To specifically identify sex-biased genes that might also affect the sex difference process, we performed a series of expression profiling to examine gene activity changes between sexes. By comparing female to male, differentially expressed transcripts (DETs) (q-value < 0.05 and log2(Fold change) > 1or log2(Fold change) < −1) were generated in four host strains (Table S4). This resulted in 8,434 differentially expressed transcripts between Caf and Cam, 7817 between Cuf and Cum, 6967 between Tof and Tom and 5151 between Cof and Com (Table S4). These results suggested that majority of the differentially expressed transcripts between sexes were female-biased. To reduce the DETs between female and male whiteflies, intersection elements of female (Figure 2A) and male (Figure 2B) specific genes were determined. Among them, 1070 female and 281 male specific genes were annotated (Table S1 and S2). The analysis of the transcriptome dynamics was shown in Figure 3. Both the female and male biased genes were enriched with members of distinct KEGG pathways and GO categories. Pathway enrichment tests revealed that pyrimidine metabolism, ribosome, mRNA surveillance pathway, ribosome biogenesis in eukaryotes, RNA polymerase, RNA degradation, DNA replication and RNA transport were enriched in female whitely. In the case of male whitefly, starch and phototransduction, lysosome, nitrogen metabolism, glycerophospholipid metabolism and phagosome were enriched (Figure S1 and S2). The enriched GO analysis for female and male biased genes were shown in Figure S3, which indicated that many enriched GO categories in female included only extracellular region while structural constituent of cuticle and hormone activity were predominant in male (Figure S3).

Bottom Line: Gender-based RNAseq data produced 27 Gb reads, and subsequent de novo assembly generated 93,948 transcripts with a N50 of 1,853 bp.A total of 1,351 differentially expressed genes were identified between male and female B. tabaci, and majority of them were female-biased.Pathway and GO enrichment experiments exhibited a gender-specific expression, including enriched translation in females, and enhanced structural constituent of cuticle in male whiteflies.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China [2].

ABSTRACT
Sex difference involving chromosomes and gene expression has been extensively documented. In this study, the gender difference in the sweetpotato whitefly Bemisia tabaci was investigated using Illumina-based transcriptomic analysis. Gender-based RNAseq data produced 27 Gb reads, and subsequent de novo assembly generated 93,948 transcripts with a N50 of 1,853 bp. A total of 1,351 differentially expressed genes were identified between male and female B. tabaci, and majority of them were female-biased. Pathway and GO enrichment experiments exhibited a gender-specific expression, including enriched translation in females, and enhanced structural constituent of cuticle in male whiteflies. In addition, a putative transformer2 gene (tra2) was cloned, and the structural feature and expression profile of tra2 were investigated. Sexually dimorphic transcriptome is an uncharted territory for the agricultural insect pests. Molecular understanding of sex determination in B. tabaci, an emerging invasive insect pest worldwide, will provide potential molecular target(s) for genetic pest control alternatives.

Show MeSH
Related in: MedlinePlus