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Use of graphene as protection film in biological environments.

Zhang W, Lee S, McNear KL, Chung TF, Lee S, Lee K, Crist SA, Ratliff TL, Zhong Z, Chen YP, Yang C - Sci Rep (2014)

Bottom Line: We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments.Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition.Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.

ABSTRACT
Corrosion of metal in biomedical devices could cause serious health problems to patients. Currently ceramics coating materials used in metal implants can reduce corrosion to some extent with limitations. Here we proposed graphene as a biocompatible protective film for metal potentially for biomedical application. We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments. Results from cell viability tests suggested that graphene greatly eliminates the toxicity of Cu by inhibiting corrosion and reducing the concentration of Cu(2+) ions produced. We demonstrated that additional thiol derivatives assembled on graphene coated Cu surface can prominently enhance durability of sole graphene protection limited by the defects in graphene film. We also demonstrated that graphene coating reduced the immune response to metal in a clinical setting for the first time through the lymphocyte transformation test. Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition. Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

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Relative cell viability of bone cells incubated with Tr-SLG/Cu, bare Cu, Tr-SLG/Cu-SH, and Cu-SH for 1 day incubation.Control is a regular cell culture without presence of any Cu sample.
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f6: Relative cell viability of bone cells incubated with Tr-SLG/Cu, bare Cu, Tr-SLG/Cu-SH, and Cu-SH for 1 day incubation.Control is a regular cell culture without presence of any Cu sample.

Mentions: To extend the application of graphene coating, we also tested the performance of mechanically transferred graphene for inhibiting corrosion of metal surface. To establish a meaningful comparison between protections by as-grown graphene on Cu substrate and by transferred graphene on the same metal, SLG grown on Cu foil was transferred to another bare Cu foil (labeled “Tr-SLG/Cu”) following the typical transferring procedure reported283335. Additional thiol coating was also performed to the transferred sample (labeled “Tr-SLG/Cu-SH”) using the same strategy discussed above. Together with Cu and Cu-SH, Tr-SLG/Cu and Tr-SLG/Cu-SH were tested for cellular experiments. As shown in Fig. 6, cell viability obtained after 1 day incubation for the Tr-SLG/Cu sample is found to be 6.4% ± 3.9%, comparable with that obtained from Cu-SH and Cu, which is likely a result of tears and rips in the graphene film produced during its transfer28. Transferred graphene, combined with additional thiol coating, offers reasonably well protection for Cu corrosion, resulting in 61.1% ± 6.2% viability. These results suggested that although it provides limited protection, transferred SLG together with additional metal surface functionalization with thiol is possible to be considered as a universal protection film preventing metal corrosion in chemical, biological and medical applications.


Use of graphene as protection film in biological environments.

Zhang W, Lee S, McNear KL, Chung TF, Lee S, Lee K, Crist SA, Ratliff TL, Zhong Z, Chen YP, Yang C - Sci Rep (2014)

Relative cell viability of bone cells incubated with Tr-SLG/Cu, bare Cu, Tr-SLG/Cu-SH, and Cu-SH for 1 day incubation.Control is a regular cell culture without presence of any Cu sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924215&req=5

f6: Relative cell viability of bone cells incubated with Tr-SLG/Cu, bare Cu, Tr-SLG/Cu-SH, and Cu-SH for 1 day incubation.Control is a regular cell culture without presence of any Cu sample.
Mentions: To extend the application of graphene coating, we also tested the performance of mechanically transferred graphene for inhibiting corrosion of metal surface. To establish a meaningful comparison between protections by as-grown graphene on Cu substrate and by transferred graphene on the same metal, SLG grown on Cu foil was transferred to another bare Cu foil (labeled “Tr-SLG/Cu”) following the typical transferring procedure reported283335. Additional thiol coating was also performed to the transferred sample (labeled “Tr-SLG/Cu-SH”) using the same strategy discussed above. Together with Cu and Cu-SH, Tr-SLG/Cu and Tr-SLG/Cu-SH were tested for cellular experiments. As shown in Fig. 6, cell viability obtained after 1 day incubation for the Tr-SLG/Cu sample is found to be 6.4% ± 3.9%, comparable with that obtained from Cu-SH and Cu, which is likely a result of tears and rips in the graphene film produced during its transfer28. Transferred graphene, combined with additional thiol coating, offers reasonably well protection for Cu corrosion, resulting in 61.1% ± 6.2% viability. These results suggested that although it provides limited protection, transferred SLG together with additional metal surface functionalization with thiol is possible to be considered as a universal protection film preventing metal corrosion in chemical, biological and medical applications.

Bottom Line: We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments.Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition.Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.

ABSTRACT
Corrosion of metal in biomedical devices could cause serious health problems to patients. Currently ceramics coating materials used in metal implants can reduce corrosion to some extent with limitations. Here we proposed graphene as a biocompatible protective film for metal potentially for biomedical application. We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments. Results from cell viability tests suggested that graphene greatly eliminates the toxicity of Cu by inhibiting corrosion and reducing the concentration of Cu(2+) ions produced. We demonstrated that additional thiol derivatives assembled on graphene coated Cu surface can prominently enhance durability of sole graphene protection limited by the defects in graphene film. We also demonstrated that graphene coating reduced the immune response to metal in a clinical setting for the first time through the lymphocyte transformation test. Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition. Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

Show MeSH
Related in: MedlinePlus