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Use of graphene as protection film in biological environments.

Zhang W, Lee S, McNear KL, Chung TF, Lee S, Lee K, Crist SA, Ratliff TL, Zhong Z, Chen YP, Yang C - Sci Rep (2014)

Bottom Line: We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments.Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition.Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.

ABSTRACT
Corrosion of metal in biomedical devices could cause serious health problems to patients. Currently ceramics coating materials used in metal implants can reduce corrosion to some extent with limitations. Here we proposed graphene as a biocompatible protective film for metal potentially for biomedical application. We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments. Results from cell viability tests suggested that graphene greatly eliminates the toxicity of Cu by inhibiting corrosion and reducing the concentration of Cu(2+) ions produced. We demonstrated that additional thiol derivatives assembled on graphene coated Cu surface can prominently enhance durability of sole graphene protection limited by the defects in graphene film. We also demonstrated that graphene coating reduced the immune response to metal in a clinical setting for the first time through the lymphocyte transformation test. Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition. Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

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Concentration of Cu2+ ions from different biological solutions.Concentrations of Cu2+ ions in (a) PBS, (b) FBS, (c) HBSS solutions and (d) cell culture media with presence of SLG/Cu (black), BLG/Cu (red) and Cu (Blue) and blank solutions as controls (Pink) measured by ICP-MS, respectively. All results are presented in a mass ratio (ppm).
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f1: Concentration of Cu2+ ions from different biological solutions.Concentrations of Cu2+ ions in (a) PBS, (b) FBS, (c) HBSS solutions and (d) cell culture media with presence of SLG/Cu (black), BLG/Cu (red) and Cu (Blue) and blank solutions as controls (Pink) measured by ICP-MS, respectively. All results are presented in a mass ratio (ppm).

Mentions: The protection effect of graphene on Cu foils in the aqueous environment was first confirmed through monitoring corrosion of Cu chemically. The corrosion of as-synthesized SLG coated Cu foils (labeled “SLG/Cu”) and bare Cu foils (labeled “Cu”) in phosphate buffered saline (PBS), fetal bovine serum (FBS), hank's balanced salt solution (HBSS), and cell culture media were tested. Corrosion of Cu was monitored through measuring the concentrations of Cu2+ ions, the primarily corrosion products generated by the reaction of Cu with these solutions34, using inductively coupled plasma-mass spectrometry (ICP-MS) on day 1, 2, 3, 4 and 5. All samples were measured in triplicate. Results are shown in Fig. 1.


Use of graphene as protection film in biological environments.

Zhang W, Lee S, McNear KL, Chung TF, Lee S, Lee K, Crist SA, Ratliff TL, Zhong Z, Chen YP, Yang C - Sci Rep (2014)

Concentration of Cu2+ ions from different biological solutions.Concentrations of Cu2+ ions in (a) PBS, (b) FBS, (c) HBSS solutions and (d) cell culture media with presence of SLG/Cu (black), BLG/Cu (red) and Cu (Blue) and blank solutions as controls (Pink) measured by ICP-MS, respectively. All results are presented in a mass ratio (ppm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3924215&req=5

f1: Concentration of Cu2+ ions from different biological solutions.Concentrations of Cu2+ ions in (a) PBS, (b) FBS, (c) HBSS solutions and (d) cell culture media with presence of SLG/Cu (black), BLG/Cu (red) and Cu (Blue) and blank solutions as controls (Pink) measured by ICP-MS, respectively. All results are presented in a mass ratio (ppm).
Mentions: The protection effect of graphene on Cu foils in the aqueous environment was first confirmed through monitoring corrosion of Cu chemically. The corrosion of as-synthesized SLG coated Cu foils (labeled “SLG/Cu”) and bare Cu foils (labeled “Cu”) in phosphate buffered saline (PBS), fetal bovine serum (FBS), hank's balanced salt solution (HBSS), and cell culture media were tested. Corrosion of Cu was monitored through measuring the concentrations of Cu2+ ions, the primarily corrosion products generated by the reaction of Cu with these solutions34, using inductively coupled plasma-mass spectrometry (ICP-MS) on day 1, 2, 3, 4 and 5. All samples were measured in triplicate. Results are shown in Fig. 1.

Bottom Line: We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments.Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition.Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.

ABSTRACT
Corrosion of metal in biomedical devices could cause serious health problems to patients. Currently ceramics coating materials used in metal implants can reduce corrosion to some extent with limitations. Here we proposed graphene as a biocompatible protective film for metal potentially for biomedical application. We confirmed graphene effectively inhibits Cu surface from corrosion in different biological aqueous environments. Results from cell viability tests suggested that graphene greatly eliminates the toxicity of Cu by inhibiting corrosion and reducing the concentration of Cu(2+) ions produced. We demonstrated that additional thiol derivatives assembled on graphene coated Cu surface can prominently enhance durability of sole graphene protection limited by the defects in graphene film. We also demonstrated that graphene coating reduced the immune response to metal in a clinical setting for the first time through the lymphocyte transformation test. Finally, an animal experiment showed the effective protection of graphene to Cu under in vivo condition. Our results open up the potential for using graphene coating to protect metal surface in biomedical application.

Show MeSH
Related in: MedlinePlus