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Antioxidant property of aerial parts and root of Phyllanthus fraternus Webster, an important medicinal plant.

Upadhyay R, Chaurasia JK, Tiwari KN, Singh K - ScientificWorldJournal (2014)

Bottom Line: Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active.Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential.Thus, P. fraternus extract can be used as potent natural antioxidant.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.

ABSTRACT
In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe³⁺-Fe⁺ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.

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Related in: MedlinePlus

Correlation between total antioxidant activity and polyphenolic (TP&TF) contents of different plant parts of P. fraternus. (a) Ethanolic extract of aerial Part. (b) Aqueous extract of aerial part. (c) Ethanolic extract of root. (d) Aqueous extract of root.
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fig3: Correlation between total antioxidant activity and polyphenolic (TP&TF) contents of different plant parts of P. fraternus. (a) Ethanolic extract of aerial Part. (b) Aqueous extract of aerial part. (c) Ethanolic extract of root. (d) Aqueous extract of root.

Mentions: Very close correlation was obtained between total antioxidant activity and polyphenolic contents (TP&TF) of various extracts which is clear from their correlation coefficient (R2) values (Figure 3). A very high linear correlation was established between antioxidant activities and TP and TF content of different extracts (R2 values ≥ 0.975).


Antioxidant property of aerial parts and root of Phyllanthus fraternus Webster, an important medicinal plant.

Upadhyay R, Chaurasia JK, Tiwari KN, Singh K - ScientificWorldJournal (2014)

Correlation between total antioxidant activity and polyphenolic (TP&TF) contents of different plant parts of P. fraternus. (a) Ethanolic extract of aerial Part. (b) Aqueous extract of aerial part. (c) Ethanolic extract of root. (d) Aqueous extract of root.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921995&req=5

fig3: Correlation between total antioxidant activity and polyphenolic (TP&TF) contents of different plant parts of P. fraternus. (a) Ethanolic extract of aerial Part. (b) Aqueous extract of aerial part. (c) Ethanolic extract of root. (d) Aqueous extract of root.
Mentions: Very close correlation was obtained between total antioxidant activity and polyphenolic contents (TP&TF) of various extracts which is clear from their correlation coefficient (R2) values (Figure 3). A very high linear correlation was established between antioxidant activities and TP and TF content of different extracts (R2 values ≥ 0.975).

Bottom Line: Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active.Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential.Thus, P. fraternus extract can be used as potent natural antioxidant.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.

ABSTRACT
In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe³⁺-Fe⁺ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.

Show MeSH
Related in: MedlinePlus