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Antioxidant property of aerial parts and root of Phyllanthus fraternus Webster, an important medicinal plant.

Upadhyay R, Chaurasia JK, Tiwari KN, Singh K - ScientificWorldJournal (2014)

Bottom Line: Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active.Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential.Thus, P. fraternus extract can be used as potent natural antioxidant.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.

ABSTRACT
In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe³⁺-Fe⁺ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.

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Total phenolic and flavonoid content in P. fraternus aerial part and root.
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fig2: Total phenolic and flavonoid content in P. fraternus aerial part and root.

Mentions: TP content, as determined by Folin-Ciocalteu's method, is reported as GAE by reference to standard curve (y = 0.026x, R2 = 0.993). Phenolic content is higher in the ethanolic extract of aerial part than its aqueous extract which is about 230.85 ± 0.59 mg/g and 161.92 ± 14.12 mg/g GAE, respectively. Similarly, ethanolic extract of root is greater in phenolic content (118.94 ± 2.69 mg/g GAE) than aqueous extract (98.37 ± 5.47 mg/g GAE) (Figure 2).


Antioxidant property of aerial parts and root of Phyllanthus fraternus Webster, an important medicinal plant.

Upadhyay R, Chaurasia JK, Tiwari KN, Singh K - ScientificWorldJournal (2014)

Total phenolic and flavonoid content in P. fraternus aerial part and root.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921995&req=5

fig2: Total phenolic and flavonoid content in P. fraternus aerial part and root.
Mentions: TP content, as determined by Folin-Ciocalteu's method, is reported as GAE by reference to standard curve (y = 0.026x, R2 = 0.993). Phenolic content is higher in the ethanolic extract of aerial part than its aqueous extract which is about 230.85 ± 0.59 mg/g and 161.92 ± 14.12 mg/g GAE, respectively. Similarly, ethanolic extract of root is greater in phenolic content (118.94 ± 2.69 mg/g GAE) than aqueous extract (98.37 ± 5.47 mg/g GAE) (Figure 2).

Bottom Line: Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active.Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential.Thus, P. fraternus extract can be used as potent natural antioxidant.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.

ABSTRACT
In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC₅₀ of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe³⁺-Fe⁺ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.

Show MeSH
Related in: MedlinePlus