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Electroacupuncture at Acupoints Reverses Plasma Glutamate, Lipid, and LDL/VLDL in an Acute Migraine Rat Model: A (1) H NMR-Based Metabolomic Study.

Gao Z, Liu X, Yu S, Zhang Q, Chen Q, Wu Q, Liu J, Sun B, Fang L, Lin J, Zhu BM, Yan X, Liang F - Evid Based Complement Alternat Med (2014)

Bottom Line: Glutamate was significantly increased (P < 0.01), whereas lipids were significantly decreased (P < 0.01) in model rats.Interestingly, the number of head-scratching and glutamate level were significantly decreased (P < 0.05) after receiving EA at both acupoints and nonacupoints.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Clinical Acupuncture and Moxibustion Department, Second School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Acupuncture and Tuina College, Chengdu University of TCM, Chengdu, Sichuan 610075, China.

ABSTRACT
Background. The objective of this study was to identify potential biomarkers of electroacupuncture (EA) on relieving acute migraine through metabolomic study. Methods. EA treatments were performed on both acupoints and nonacupoints on the nitroglycerin (NTG)-induced migraine rat model. NMR experiments and multivariate analysis were used for metabolomic analysis. Results. The number of head-scratching, the main ethology index of migraine rat model, was significantly increased (P < 0.01) after NTG injection. The plasma metabolic profile of model group was distinct from that of the control group. Glutamate was significantly increased (P < 0.01), whereas lipids were significantly decreased (P < 0.01) in model rats. After EA at acupoints, the metabolic profile of model rats was normalized, with decreased glutamate (P < 0.05) and increased lipids (P < 0.01). In contrast, EA at nonacupoints did not restore the metabolic profile, but with six metabolites significantly different from acupoints group. Interestingly, the number of head-scratching and glutamate level were significantly decreased (P < 0.05) after receiving EA at both acupoints and nonacupoints. Conclusions. EA at acupoints may relieve acute migraine by restoring the plasma metabolic profile and plasma glutamate, while EA at nonacupoints may modestly relieve acute migraine by decreasing plasma glutamate.

No MeSH data available.


Related in: MedlinePlus

Trial fluidogram.
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fig1: Trial fluidogram.

Mentions: The overall experimental approach is shown in Figure 1. Fifty female Sprague Dawley rats, 3 months old and weighing 160 ± 20 g, were purchased from the Experimental Animal Center of Chengdu University of Traditional Chinese Medicine. Rats were housed in a well-ventilated colony room at a temperature of 15–22°C and a relative humidity of 50–70% on a 12 h light/12 h dark cycle. Food and tap water were provided ad libitum. Rats were allowed to acclimatize for one week before experiments began and then were randomly divided into 4 groups using an SPSS randomization process: control (untreated, without any treatment), NTG (nitroglycerin-treated model), EA (electroacupuncture at acupoints following NTG-treatment), and NA (electroacupuncture at nonacupoints following NTG-treatment). Each group has 10 rats and was fed separately in two metabolic cages.


Electroacupuncture at Acupoints Reverses Plasma Glutamate, Lipid, and LDL/VLDL in an Acute Migraine Rat Model: A (1) H NMR-Based Metabolomic Study.

Gao Z, Liu X, Yu S, Zhang Q, Chen Q, Wu Q, Liu J, Sun B, Fang L, Lin J, Zhu BM, Yan X, Liang F - Evid Based Complement Alternat Med (2014)

Trial fluidogram.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921982&req=5

fig1: Trial fluidogram.
Mentions: The overall experimental approach is shown in Figure 1. Fifty female Sprague Dawley rats, 3 months old and weighing 160 ± 20 g, were purchased from the Experimental Animal Center of Chengdu University of Traditional Chinese Medicine. Rats were housed in a well-ventilated colony room at a temperature of 15–22°C and a relative humidity of 50–70% on a 12 h light/12 h dark cycle. Food and tap water were provided ad libitum. Rats were allowed to acclimatize for one week before experiments began and then were randomly divided into 4 groups using an SPSS randomization process: control (untreated, without any treatment), NTG (nitroglycerin-treated model), EA (electroacupuncture at acupoints following NTG-treatment), and NA (electroacupuncture at nonacupoints following NTG-treatment). Each group has 10 rats and was fed separately in two metabolic cages.

Bottom Line: Glutamate was significantly increased (P < 0.01), whereas lipids were significantly decreased (P < 0.01) in model rats.Interestingly, the number of head-scratching and glutamate level were significantly decreased (P < 0.05) after receiving EA at both acupoints and nonacupoints.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Clinical Acupuncture and Moxibustion Department, Second School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Acupuncture and Tuina College, Chengdu University of TCM, Chengdu, Sichuan 610075, China.

ABSTRACT
Background. The objective of this study was to identify potential biomarkers of electroacupuncture (EA) on relieving acute migraine through metabolomic study. Methods. EA treatments were performed on both acupoints and nonacupoints on the nitroglycerin (NTG)-induced migraine rat model. NMR experiments and multivariate analysis were used for metabolomic analysis. Results. The number of head-scratching, the main ethology index of migraine rat model, was significantly increased (P < 0.01) after NTG injection. The plasma metabolic profile of model group was distinct from that of the control group. Glutamate was significantly increased (P < 0.01), whereas lipids were significantly decreased (P < 0.01) in model rats. After EA at acupoints, the metabolic profile of model rats was normalized, with decreased glutamate (P < 0.05) and increased lipids (P < 0.01). In contrast, EA at nonacupoints did not restore the metabolic profile, but with six metabolites significantly different from acupoints group. Interestingly, the number of head-scratching and glutamate level were significantly decreased (P < 0.05) after receiving EA at both acupoints and nonacupoints. Conclusions. EA at acupoints may relieve acute migraine by restoring the plasma metabolic profile and plasma glutamate, while EA at nonacupoints may modestly relieve acute migraine by decreasing plasma glutamate.

No MeSH data available.


Related in: MedlinePlus