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YgaE regulates out membrane proteins in Salmonella enterica serovar Typhi under hyperosmotic stress.

Wang M, Feng P, Chen X, Zhang H, Ni B, Xie X, Du H - ScientificWorldJournal (2014)

Bottom Line: Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress.Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S.Typhi.

View Article: PubMed Central - PubMed

Affiliation: Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

ABSTRACT
Salmonella enterica serovar Typhi (S. Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that ygaE was the repressor of gab operon in S. Typhi as in Escherichia coli (E. coli), though the sequence of ygaE is totally different from gabC (formerly ygaE) in E. coli. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in S. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S. Typhi.

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The gab operon structure in S. Typhi. The arrowhead represents the length of the gene; the arrowhead of ygaE corresponds to 0.678 kb.
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fig1: The gab operon structure in S. Typhi. The arrowhead represents the length of the gene; the arrowhead of ygaE corresponds to 0.678 kb.

Mentions: In E. coli, gabT codes for a GABA transaminase that generates succinic semialdehyde. gabD specifies an NADP-dependent succinic semialdehyde dehydrogenase, which oxidizes succinic semialdehyde to succinate [30]. GabC does not obviously respond to a specific inducer. GabC is in the FadR subfamily of the GntR family of transcriptional regulators [31]. In S. Typhi, gabD encodes for a succinate-semialdehyde dehydrogenase, gabT encodes for a 4-aminobutyrate aminotransferase, and ygaF is a putative GAB DTP gene cluster repressor (Table 3). We compared the sequences of gab operon of S. Typhi to that of E. coli and found they are about 80% homologous. However, despite the same regulation pattern to gab operon, the sequence of ygaE in S. Typhi is totally different from gabC in E. coli, which also indicates that ygaE in S. Typhi may play other roles that is not found in E. coli. The gene organization of gab operon in S. Typhi was shown in Figure 1. Our microarray results suggested that YgaE can response to osmotic pressure in early stage to repress the expression of gab operon. However, the concrete regulation mechanism, whether ygaF is included in the gab operon and the functions of gab operon in S. Typhi, still needs further study.


YgaE regulates out membrane proteins in Salmonella enterica serovar Typhi under hyperosmotic stress.

Wang M, Feng P, Chen X, Zhang H, Ni B, Xie X, Du H - ScientificWorldJournal (2014)

The gab operon structure in S. Typhi. The arrowhead represents the length of the gene; the arrowhead of ygaE corresponds to 0.678 kb.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921978&req=5

fig1: The gab operon structure in S. Typhi. The arrowhead represents the length of the gene; the arrowhead of ygaE corresponds to 0.678 kb.
Mentions: In E. coli, gabT codes for a GABA transaminase that generates succinic semialdehyde. gabD specifies an NADP-dependent succinic semialdehyde dehydrogenase, which oxidizes succinic semialdehyde to succinate [30]. GabC does not obviously respond to a specific inducer. GabC is in the FadR subfamily of the GntR family of transcriptional regulators [31]. In S. Typhi, gabD encodes for a succinate-semialdehyde dehydrogenase, gabT encodes for a 4-aminobutyrate aminotransferase, and ygaF is a putative GAB DTP gene cluster repressor (Table 3). We compared the sequences of gab operon of S. Typhi to that of E. coli and found they are about 80% homologous. However, despite the same regulation pattern to gab operon, the sequence of ygaE in S. Typhi is totally different from gabC in E. coli, which also indicates that ygaE in S. Typhi may play other roles that is not found in E. coli. The gene organization of gab operon in S. Typhi was shown in Figure 1. Our microarray results suggested that YgaE can response to osmotic pressure in early stage to repress the expression of gab operon. However, the concrete regulation mechanism, whether ygaF is included in the gab operon and the functions of gab operon in S. Typhi, still needs further study.

Bottom Line: Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress.Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S.Typhi.

View Article: PubMed Central - PubMed

Affiliation: Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

ABSTRACT
Salmonella enterica serovar Typhi (S. Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that ygaE was the repressor of gab operon in S. Typhi as in Escherichia coli (E. coli), though the sequence of ygaE is totally different from gabC (formerly ygaE) in E. coli. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in S. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S. Typhi.

Show MeSH
Related in: MedlinePlus