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Two-component signal transduction system SaeRS positively regulates Staphylococcus epidermidis glucose metabolism.

Lou Q, Qi Y, Ma Y, Qu D - ScientificWorldJournal (2014)

Bottom Line: Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated.The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted.The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, China.

ABSTRACT
Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/lonization mass spectrometry (MALDI-TOF-MS). Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated. The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted. The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS.

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Related in: MedlinePlus

Carbohydrate metabolic pathways in the saeRS mutant under aerobic conditions. The following enzymes (identified in the 2-DE experiment) were found at lower levels in the saeRS mutant: fructose bisphosphate aldolase, glyceraldehyde 3-phosphate dehydrogenase, enolase, pyruvate kinase, succinyl-CoA synthase, and isocitrate dehydrogenase. Phosphate acetyltransferase was found at higher level in the saeRS mutant.
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fig2: Carbohydrate metabolic pathways in the saeRS mutant under aerobic conditions. The following enzymes (identified in the 2-DE experiment) were found at lower levels in the saeRS mutant: fructose bisphosphate aldolase, glyceraldehyde 3-phosphate dehydrogenase, enolase, pyruvate kinase, succinyl-CoA synthase, and isocitrate dehydrogenase. Phosphate acetyltransferase was found at higher level in the saeRS mutant.

Mentions: Approximately 40% of the identified proteins were defined as enzymes. Most of the identified enzymes were upregulated at the postexponential phase and categorized as involved in glucose metabolism. Figure 2 showed that enzymes related to glycolysis (fructose-bisphosphate aldolase (GI: 27469074), glyceraldehyde 3-phosphate dehydrogenase (GI: 27467475), enolase (GI: 27467479), pyruvate kinase (GI: 27468291)), the TCA cycle (succinyl-CoA synthase (GI: 27467842), and isocitrate dehydrogenase (GI: 27468288)) were downregulated at the post-exponential phase. This is the first report that the expression of enzymes related to carbohydrate metabolism in Staphylococcus species is down-regulated by deletion of TCS SaeRS.


Two-component signal transduction system SaeRS positively regulates Staphylococcus epidermidis glucose metabolism.

Lou Q, Qi Y, Ma Y, Qu D - ScientificWorldJournal (2014)

Carbohydrate metabolic pathways in the saeRS mutant under aerobic conditions. The following enzymes (identified in the 2-DE experiment) were found at lower levels in the saeRS mutant: fructose bisphosphate aldolase, glyceraldehyde 3-phosphate dehydrogenase, enolase, pyruvate kinase, succinyl-CoA synthase, and isocitrate dehydrogenase. Phosphate acetyltransferase was found at higher level in the saeRS mutant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921950&req=5

fig2: Carbohydrate metabolic pathways in the saeRS mutant under aerobic conditions. The following enzymes (identified in the 2-DE experiment) were found at lower levels in the saeRS mutant: fructose bisphosphate aldolase, glyceraldehyde 3-phosphate dehydrogenase, enolase, pyruvate kinase, succinyl-CoA synthase, and isocitrate dehydrogenase. Phosphate acetyltransferase was found at higher level in the saeRS mutant.
Mentions: Approximately 40% of the identified proteins were defined as enzymes. Most of the identified enzymes were upregulated at the postexponential phase and categorized as involved in glucose metabolism. Figure 2 showed that enzymes related to glycolysis (fructose-bisphosphate aldolase (GI: 27469074), glyceraldehyde 3-phosphate dehydrogenase (GI: 27467475), enolase (GI: 27467479), pyruvate kinase (GI: 27468291)), the TCA cycle (succinyl-CoA synthase (GI: 27467842), and isocitrate dehydrogenase (GI: 27468288)) were downregulated at the post-exponential phase. This is the first report that the expression of enzymes related to carbohydrate metabolism in Staphylococcus species is down-regulated by deletion of TCS SaeRS.

Bottom Line: Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated.The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted.The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, China.

ABSTRACT
Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/lonization mass spectrometry (MALDI-TOF-MS). Of 55 identified proteins that significantly differed in expression between the two strains, 15 were upregulated and 40 were downregulated. The downregulated proteins included enzymes related to glycolysis and TCA cycle, suggesting that glucose is not properly utilized in S. epidermidis when saeRS was deleted. The study will be helpful for treatment of S. epidermidis infection from the viewpoint of metabolic modulation dependent on two-component signal transduction system SaeRS.

Show MeSH
Related in: MedlinePlus