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Nuclear Morphometric Analysis of Leydig Cells of Male Pubertal Rats Exposed In Utero to Di(n-butyl) Phthalate.

Wakui S, Motohashi M, Satoh T, Shirai M, Mutou T, Takahashi H, Wempe MF, Endou H, Inomata T, Asari M - J Toxicol Pathol (2013)

Bottom Line: Nuclear pleomorphism of hyperplastic LCs is common and is considered to be continuous progressive degeneration.The results of the 5-wk-old DBP group were similar to those of the vehicle group; LC nuclei of the 7-wk-old DBP group showed normal ploidy and similar amounts of DNA.However, the size, elongation and peripheral chromatin aggregation parameters were significantly higher, and the reticular chromatin distribution and isolated chromatin aggregation parameters were significantly lower compared with the vehicle group.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.

ABSTRACT
We recently reported that prenatal rat exposure to di(n-butyl) phthalate (DBP) induced Leydig cell (LC) hyperplasia after nine weeks (wks) of age, yet the number of LCs was similar to that of the vehicle group until seven weeks. Nuclear pleomorphism of hyperplastic LCs is common and is considered to be continuous progressive degeneration. Thus, computer-assisted image cell nuclear analysis of LCs was performed on 5- and 7-wk-old Sprague-Dawley (SD) rats whose dams had been administered DBP (i.g.) at 100 mg/kg/day or vehicle (corn oil) on gestation day 12 to 21. The results of the 5-wk-old DBP group were similar to those of the vehicle group; LC nuclei of the 7-wk-old DBP group showed normal ploidy and similar amounts of DNA. However, the size, elongation and peripheral chromatin aggregation parameters were significantly higher, and the reticular chromatin distribution and isolated chromatin aggregation parameters were significantly lower compared with the vehicle group. The present study quantitatively demonstrated nuclear morphological alterations in rat LCs at 7 wks old (puberty) due to the prenatal DBP administration before apparent LC hyperplasia developed.

No MeSH data available.


Related in: MedlinePlus

Representative DNA histograms: distribution of DNA mass generated by CAS 200 computer-assisted cytometry of the 7-wk-old vehicle group (A) and 7-wk-old DBP group (B). Cells that contain normal amounts of DNA [2C]; cells in the G2/M-phase area of the cell cycle [4C].
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fig_002: Representative DNA histograms: distribution of DNA mass generated by CAS 200 computer-assisted cytometry of the 7-wk-old vehicle group (A) and 7-wk-old DBP group (B). Cells that contain normal amounts of DNA [2C]; cells in the G2/M-phase area of the cell cycle [4C].

Mentions: The cell nuclear analysis parameters of LCs of the 5-wk-old DBP group shown in Table 1 were not significantly different from those of the vehicle group (Table 2Table 2. Summary of Morphometrical Parameters Obtained by Extracting with a CAS 200TM Image Analyzer and Analysis by Cell-SheetTM of Leydig Cell Nuclei in 5-wk-old Rats). Although the DNA ploidy of LCs of the 7-wk-old DBP group and that of the vehicle group were the diploid type (Fig. 2Fig. 2.


Nuclear Morphometric Analysis of Leydig Cells of Male Pubertal Rats Exposed In Utero to Di(n-butyl) Phthalate.

Wakui S, Motohashi M, Satoh T, Shirai M, Mutou T, Takahashi H, Wempe MF, Endou H, Inomata T, Asari M - J Toxicol Pathol (2013)

Representative DNA histograms: distribution of DNA mass generated by CAS 200 computer-assisted cytometry of the 7-wk-old vehicle group (A) and 7-wk-old DBP group (B). Cells that contain normal amounts of DNA [2C]; cells in the G2/M-phase area of the cell cycle [4C].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921929&req=5

fig_002: Representative DNA histograms: distribution of DNA mass generated by CAS 200 computer-assisted cytometry of the 7-wk-old vehicle group (A) and 7-wk-old DBP group (B). Cells that contain normal amounts of DNA [2C]; cells in the G2/M-phase area of the cell cycle [4C].
Mentions: The cell nuclear analysis parameters of LCs of the 5-wk-old DBP group shown in Table 1 were not significantly different from those of the vehicle group (Table 2Table 2. Summary of Morphometrical Parameters Obtained by Extracting with a CAS 200TM Image Analyzer and Analysis by Cell-SheetTM of Leydig Cell Nuclei in 5-wk-old Rats). Although the DNA ploidy of LCs of the 7-wk-old DBP group and that of the vehicle group were the diploid type (Fig. 2Fig. 2.

Bottom Line: Nuclear pleomorphism of hyperplastic LCs is common and is considered to be continuous progressive degeneration.The results of the 5-wk-old DBP group were similar to those of the vehicle group; LC nuclei of the 7-wk-old DBP group showed normal ploidy and similar amounts of DNA.However, the size, elongation and peripheral chromatin aggregation parameters were significantly higher, and the reticular chromatin distribution and isolated chromatin aggregation parameters were significantly lower compared with the vehicle group.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Laboratory Animal Science, and Veterinary Anatomy, Azabu University School of Veterinary Medicine, 1-17-71 Chuo, Sagamihara, Kanagawa 252-5201, Japan.

ABSTRACT
We recently reported that prenatal rat exposure to di(n-butyl) phthalate (DBP) induced Leydig cell (LC) hyperplasia after nine weeks (wks) of age, yet the number of LCs was similar to that of the vehicle group until seven weeks. Nuclear pleomorphism of hyperplastic LCs is common and is considered to be continuous progressive degeneration. Thus, computer-assisted image cell nuclear analysis of LCs was performed on 5- and 7-wk-old Sprague-Dawley (SD) rats whose dams had been administered DBP (i.g.) at 100 mg/kg/day or vehicle (corn oil) on gestation day 12 to 21. The results of the 5-wk-old DBP group were similar to those of the vehicle group; LC nuclei of the 7-wk-old DBP group showed normal ploidy and similar amounts of DNA. However, the size, elongation and peripheral chromatin aggregation parameters were significantly higher, and the reticular chromatin distribution and isolated chromatin aggregation parameters were significantly lower compared with the vehicle group. The present study quantitatively demonstrated nuclear morphological alterations in rat LCs at 7 wks old (puberty) due to the prenatal DBP administration before apparent LC hyperplasia developed.

No MeSH data available.


Related in: MedlinePlus