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Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet.

Abiko Y, Kojima T, Murata M, Tsujiwaki M, Takeuchi M, Sawada N, Mori M - J Toxicol Pathol (2013)

Bottom Line: In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells.On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal.These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

View Article: PubMed Central - PubMed

Affiliation: Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan.

ABSTRACT
DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

No MeSH data available.


Related in: MedlinePlus

(A) H.E. staining of the kidney in mice fed the DDC diet for 12 weeks (b) and the control (a). Bars=50 μm. (B) Immunohistochemistry for claudin-1 (CLDN-1), claudin-2 (CLDN-2), claudin-8 (CLDN-8) and claudin-19 (CLDN-19) in the kidney of mice fed the DDC diet for 12 weeks (b) and the control (a), Bars=100 μm. Arrows in control mouse kidney (a) show typical immunohistochemical localization of claudin subspecies in the epithelial cells of the Bowman’s capsule (CLDN-1) and renal tubules (CLDN-2, CLDN-8 and CLDN-19). Arrows in the mouse kidney designated (b) show that of claudins in approximately identical portions of kidneys of a mouse fed the DDC diet for 12 weeks.
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fig_003: (A) H.E. staining of the kidney in mice fed the DDC diet for 12 weeks (b) and the control (a). Bars=50 μm. (B) Immunohistochemistry for claudin-1 (CLDN-1), claudin-2 (CLDN-2), claudin-8 (CLDN-8) and claudin-19 (CLDN-19) in the kidney of mice fed the DDC diet for 12 weeks (b) and the control (a), Bars=100 μm. Arrows in control mouse kidney (a) show typical immunohistochemical localization of claudin subspecies in the epithelial cells of the Bowman’s capsule (CLDN-1) and renal tubules (CLDN-2, CLDN-8 and CLDN-19). Arrows in the mouse kidney designated (b) show that of claudins in approximately identical portions of kidneys of a mouse fed the DDC diet for 12 weeks.

Mentions: In the kidneys of mice fed the DDC diet for 12 weeks, H.E. staining revealed that some glomeruli were slightly enlarged compared with the control, whereas no change was observed in renal tubules (Fig. 3AFig. 3.


Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet.

Abiko Y, Kojima T, Murata M, Tsujiwaki M, Takeuchi M, Sawada N, Mori M - J Toxicol Pathol (2013)

(A) H.E. staining of the kidney in mice fed the DDC diet for 12 weeks (b) and the control (a). Bars=50 μm. (B) Immunohistochemistry for claudin-1 (CLDN-1), claudin-2 (CLDN-2), claudin-8 (CLDN-8) and claudin-19 (CLDN-19) in the kidney of mice fed the DDC diet for 12 weeks (b) and the control (a), Bars=100 μm. Arrows in control mouse kidney (a) show typical immunohistochemical localization of claudin subspecies in the epithelial cells of the Bowman’s capsule (CLDN-1) and renal tubules (CLDN-2, CLDN-8 and CLDN-19). Arrows in the mouse kidney designated (b) show that of claudins in approximately identical portions of kidneys of a mouse fed the DDC diet for 12 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921928&req=5

fig_003: (A) H.E. staining of the kidney in mice fed the DDC diet for 12 weeks (b) and the control (a). Bars=50 μm. (B) Immunohistochemistry for claudin-1 (CLDN-1), claudin-2 (CLDN-2), claudin-8 (CLDN-8) and claudin-19 (CLDN-19) in the kidney of mice fed the DDC diet for 12 weeks (b) and the control (a), Bars=100 μm. Arrows in control mouse kidney (a) show typical immunohistochemical localization of claudin subspecies in the epithelial cells of the Bowman’s capsule (CLDN-1) and renal tubules (CLDN-2, CLDN-8 and CLDN-19). Arrows in the mouse kidney designated (b) show that of claudins in approximately identical portions of kidneys of a mouse fed the DDC diet for 12 weeks.
Mentions: In the kidneys of mice fed the DDC diet for 12 weeks, H.E. staining revealed that some glomeruli were slightly enlarged compared with the control, whereas no change was observed in renal tubules (Fig. 3AFig. 3.

Bottom Line: In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells.On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal.These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

View Article: PubMed Central - PubMed

Affiliation: Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan.

ABSTRACT
DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

No MeSH data available.


Related in: MedlinePlus