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Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet.

Abiko Y, Kojima T, Murata M, Tsujiwaki M, Takeuchi M, Sawada N, Mori M - J Toxicol Pathol (2013)

Bottom Line: In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells.On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal.These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

View Article: PubMed Central - PubMed

Affiliation: Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan.

ABSTRACT
DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry for claudin-3 (CLDN-3), claudin-7 (CLDN-7) and claudin-15 (CLDN-15) of the jejunum in mice fed the DDC diet for 12 weeks with mild injury (b: mild) and severe injury (c: severe) and the control (a). Bars=200 μm. C: crypts. V: villi.
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fig_002: Immunohistochemistry for claudin-3 (CLDN-3), claudin-7 (CLDN-7) and claudin-15 (CLDN-15) of the jejunum in mice fed the DDC diet for 12 weeks with mild injury (b: mild) and severe injury (c: severe) and the control (a). Bars=200 μm. C: crypts. V: villi.

Mentions: In the control mouse jejunum, claudin-3 and claudin-15 were expressed in the apical-most region in villous and crypt epithelia, whereas claudin-7 was observed throughout the lateral membrane of villous and crypt epithelia (Fig. 2Fig. 2.


Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet.

Abiko Y, Kojima T, Murata M, Tsujiwaki M, Takeuchi M, Sawada N, Mori M - J Toxicol Pathol (2013)

Immunohistochemistry for claudin-3 (CLDN-3), claudin-7 (CLDN-7) and claudin-15 (CLDN-15) of the jejunum in mice fed the DDC diet for 12 weeks with mild injury (b: mild) and severe injury (c: severe) and the control (a). Bars=200 μm. C: crypts. V: villi.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921928&req=5

fig_002: Immunohistochemistry for claudin-3 (CLDN-3), claudin-7 (CLDN-7) and claudin-15 (CLDN-15) of the jejunum in mice fed the DDC diet for 12 weeks with mild injury (b: mild) and severe injury (c: severe) and the control (a). Bars=200 μm. C: crypts. V: villi.
Mentions: In the control mouse jejunum, claudin-3 and claudin-15 were expressed in the apical-most region in villous and crypt epithelia, whereas claudin-7 was observed throughout the lateral membrane of villous and crypt epithelia (Fig. 2Fig. 2.

Bottom Line: In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells.On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal.These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

View Article: PubMed Central - PubMed

Affiliation: Sapporo General Pathology Laboratory Co., Ltd., 3-17, S12 W18, Sapporo 064-0912, Japan.

ABSTRACT
DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells.

No MeSH data available.


Related in: MedlinePlus